[The analysis of content of lymphocytes carrying adhesion molecules in patients with psoriasis.]

The study of mechanisms of migration activity of lymphocytes into skin under psoriasis represents both theoretical and practical interest because regulation of the given process provides new possibility in therapy. As is well known, process of migration of lymphocytes through endothelium of micro-circulation is regulated by molecules of adhesion. The study was carried out to detect content of lymphocytes expressing molecules of adhesion depending on their morphology. The study was implemented in 82 patients with vulgar and exudative psoriasis at progressing and stationary stages (39 females and 43 males out of total number) aged from 20 to 60 years. The control group consisted of 50 healthy individuals (28 females and 22 males). The lymphocytogram was analyzed alongside with detection of content of lymphocytes expressing receptors to molecules of adhesion in venous blood. The content of minor lymphocytes had no alteration in condition of hyper-proliferation of keratinocytes. This occurrence is typical for psoriasis. In patients with psoriasis, no alteration of concentration of medium lymphocytes was detected. Under psoriasis, intensified migration of large lymphocytes from peripheral blood is observed testified by decreasing of their absolute number and statistically reliable correlations with content of lymphocytes expressing receptors to LFA-1, LFA-3, ICAM-1 и PECAM-1.

[THE LEVEL OF EXPRESSION OF MOLECULES OF ADHESION ON LYMPHOCYTES DEPENDING ON AMOUNT OF THEIR CYTOPLASM].

The lymphocytes are true immunocytes specialized in discerning antigen in organism. Their behavior in blood is regulated by several classes of adhesion proteins, including selectin, integrin, immunoglobulin. In healthy humans there is no data concerning level of expression of adhesion molecules on lymphocytes depending on size of their cytoplasm. The study was carried out to determine level of expression of adhesion molecules of lymphocytes depending on size of their cytoplasm. The flow cytometer was applied to determine in venous blood level of expression of adhesion molecules in 50 individuals (22 males and 28 females) aged from 20 to 60 years and having no chronic pathology in anamnesis. The analysis of lymphocytogram permitted to differentiate lymphocytes according volume of cell considering size of cytoplasm: small lymphocytes- up to 8 mkm; medium - from 8 to 12 mkm; large - more than 12 mkm. In males a tendency was established concerning decreasing of concentration of lymphocytes with expressed molecule of L-selectin. The absence was detected concerning gender differences in level of lymphocytes with receptor LFA-1 and also lymphocytes with molecule ICAM-1. In males concentration of lymphocytes with receptor LFA-3 was higher than in females but only as a tendency. The lower level of expression of molecule PECAM-1 in males was observed. The correlation analysis between level of expresion of adhesion molecules and concentration of lymphocytes differing in size of cytoplasm, demonstrated that at increasing of size of cytoplasm of lymphocytes increases number of statistically reliable correlations. The shedding of molecules of L-selectin in lymphocytes proceeds significantly more active than in monocytes. At that, medium plasma lymphocytes and large granular lymphocytes identified as natural killers are more predisposed to migration. However, lymphocytes entering condition of lympho-proliferation have less ability to adhesion.

[The level of expression of molecules of adhesion on monocytes depending on morphological differentiation of nuclei].

The article demonstrates that in conditions of absence of antigen stimulation in venous blood percentage of promocytes made up 26% (20.0; 36.0), monocytes proper--40% (29.5; 47.0), polymorphic nuclear monocytes--31.0% (24.5; 43.0). The molecule of L-selectin was expressed by 42.2% of monocytes; of LFA-1--99.72%; of ICAM-1--88.88%; of LFA-3--94.11%; of PECAM-1--97.50% of monocytes. The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. This occurrence can testify readiness to implementation of phase of sliding, solid adhesion and transmigration immediately by polymorphic nuclear monocytes primarily.

[The expression level of adhesion molecules on neutrophils depending at segmentation of their nuclei].

The article deals with results of detection of expression level of adhesion molecules on neutrophils and segmentation of their nuclei. It is established that in conditions of absence of antigen stimulation neutrophils of circulating pool express molecules of L-selectin in 53.34%, LFA-1 molecules in 65.64%, ICAM-1 in 40.51%, LE4-3 in 58.72% and PECAM-1 in 59.74%. The full readiness to realization of phase of sliding, strong adhesion and immediately transmigration itselfis detected in neutrophils with five segments in nucleus.

[Ratio of haematology indexes in exudation of "skin window" and venous blood].

The data obtained revealed the maintenance of basophiles; monocytes and large granular lymphocytes were higher in the skin then in the venous blood. The level of eosinophiles and lymphocytes were lower in the skin. Concentrations of the polymorphic leucocytes were equal in the skin and in the blood.

[Immunologic characteristics of individuals working on the archipelago Spitzbergen]. / Immunologicheskie osobennosti u rabotaiushchikh na apkhipelage Shpitsbergen.

A complex effect with photo-periodic components and depending on the duration of stay on Shpitzbergen Archipelago of people working there, was revealed in respect to the serum concentration of Ig A, G, M. The monocytes, autoantibodies, and circular immune complexes were increased, whereas lymphocytes, T-cells, and natural killers were decreased in winter time.