[Changes in the phospholipid composition of mitochondria during heat incubation]. / Izmenenie fosfolipidnogo sostava mitokhondrii pri teplovoi inkubatsii.

Incubation of liver mitochondria at 37 degrees C causes changes in the phospholipid composition, such as the decrease in the levels of major phospholipids (e.g. phosphatidylcholine, phosphatidylethanolamine, cardiolipin) and their lysoderivatives as well as an increase in the levels of phosphatidic and lysophosphatidic acids. Similar changes in the phospholipid composition are observed upon heat incubation of mitochondrial fragments ("ghosts", inner and outer mitochondrial membranes). Ca2+ accelerate the heat-induced changes in the phospholipid levels resulting from heat incubation, whereas EGTA, in contrast, decelerates them. The role of an endogenous system of lipolytic enzymes in the observed conversions of mitochondrial phospholipids is discussed.

[Erythrocyte lipids in diabetes mellitus with vascular involvement]. / Lipidy éritrotsitov pri sakharnom diabete s sosudistymi porazheniiami.

Alterations in content of neutral lipids, phospholipids and of their lysoforms were studied in erythrocyte membranes of patients with various severity of vascular impairments considering clinical manifestation of diabetes mellitus. Impairments of lipid composition in erythrocyte membranes observed correlated with the steps of diabetic angiopathies, thus considering an importance of these alterations in pathogenesis of vascular diseases of patients with diabetes mellitus.

[Formation of phosphatidylethanol in alcoholic intoxication]. / Obrazovanie fosfatidilétanola pri alkogol'noi intoksikatsii.

Phospholipid composition was studied in membranes of various tissues as well as of mitochondria and erythrocytes of rats with alcohol intoxication. A new phospholipid--phosphatidyl ethanol, which was absent in tissues of control animals, was detected in liver, heart, kidney, small intestine tissues as well as in erythrocytes and liver mitochondria of rats consuming ethanol independently on the alcohol administration procedure used. Studies of rat liver mitochondria and mitochondrial "ghosts" showed that formation of phosphatidyl ethanol and phosphatidic acid was catalyzed by membranal endogenous phospholipase D. Phosphatidyl ethanol appears to be of importance in membranotropic effect of ethanol--one of main factors participating in alcohol pathology development. This phospholipid was found also in erythrocytes and blood of patients with chronic alcoholism, which were subjected to alcohol provocation before examination. In erythrocytes of the other group of patients with chronic alcoholism, not provocated with alcohol, the phosphatidyl ethanol fraction was not found but content of phosphatidyl ethanolamine was distinctly increased. In patients with delirium tremens phosphatidyl ethanol was not observed, content of phosphatidyl ethanolamine was not increased, while amount of phosphatidic acid was considerably elevated.

[Lipid composition of thrombocytes in diabetes mellitus depending on the degree of microangiopathy]. / Lipidnyi sostav trombotsitov pri sakharnom diabete v zavisimosti ot stepeni mikroangiopatii.

The paper is concerned with the results of a study of the content of platelet lipid fractions in 115 patients with type I and II diabetes mellitus with a different degree of vascular complications. The results showed that the development of vascular lesions caused the appearance of specific disorders of platelet lipid composition characteristic for type I and II diabetes mellitus. In type I disorders of neutral phospholipids and free fatty acids were noted whereas in type II the above changes were noted in parallel with changes in the levels of cholesterol and acid phospholipids. With an increase in vascular lesions in type II there was a significant increase in phospholipase A2 activity, in type I under the same conditions the activity of this enzyme was absent. These facts suggest correlation between the development of vascular complications of diabetes mellitus and disorders in platelet membrane lipid structure.