RESUMO
The effect of cervical spinal cord injury (CSCI) on oxidative stress parameters was assessed. The study was conducted in 42 patients with CSCI (studied group), 15 patients with cerebral concussion, without CSCI (Control II), and 30 healthy volunteers (Control I). Blood was taken from the basilic vein: before and seven days after the spinal cord decompression surgery (mean time from CSCI to surgery: 8 hours) in the studied group and once in the controls. Thiobarbituric acid reactive substances (TBARS) and conjugated dienes (CD) concentrations, and glutathione peroxidase (GPx), catalase (CAT), and creatine kinase (CK) activities before the surgery were higher in the studied group than in the controls. Reduced glutathione concentration was similar in all groups. Superoxide dismutase (SOD) in the studied group was 16% lower (P ≤ 0.001) than in Control I. Lipid peroxidation products, and GPx and CAT activities in erythrocytes seven days after the surgery were lower (P ≤ 0.001), while SOD was 25% higher (P ≤ 0.001) than before the surgery. CK in blood plasma after the surgery was 34% lower (P ≤ 0.001) than before it. CSCI is accompanied by oxidative stress. Surgical and pharmacological treatment helps to restore the oxidant-antioxidant balance.
Assuntos
Antioxidantes/metabolismo , Medula Cervical/lesões , Oxigênio/sangue , Traumatismos da Medula Espinal/sangue , Adulto , Concussão Encefálica/sangue , Catalase/metabolismo , Creatina Quinase/metabolismo , Eritrócitos/citologia , Feminino , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Voluntários Saudáveis , Humanos , Peroxidação de Lipídeos , Masculino , Oxidantes/química , Estresse Oxidativo , Análise de Regressão , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido TiobarbitúricoRESUMO
The aim of the study was to evaluate the intensity of oxygen-derived free radicals generation (expressed as the lipid peroxidation process), as well as antioxidant potential changes in patients with cervical spinal cord injury. Subjects in the study were 32 patients treated in the Neurosurgery and Neurotraumatology Clinic, Medical University in Bydgoszcz. Malondialdehyde (MDA) concentration and conjugated dienes (CD) level in the patients' blood plasma and erythrocytes, as well as their plasma antioxidant potential in reaction with a stable free radical--1.1-diphenyl-2-picryl-hydrazyl were assayed. Intensification of the lipid peroxidation process as well as a decrement of plasma antioxidant potential were found in the patients with cervical spinal cord injury. A strong correlation was also shown between severity of cervical spinal cord injury assessed using the ASIA impairment scale on the one hand and intensification of the lipid peroxidation process and plasma antioxidant potential decrease on the other hand.
Assuntos
Antioxidantes/metabolismo , Vértebras Cervicais , Radicais Livres/sangue , Peroxidação de Lipídeos , Peróxidos Lipídicos/sangue , Traumatismos da Medula Espinal/sangue , Adulto , Análise de Variância , Compostos de Bifenilo , Estudos de Casos e Controles , Feminino , Humanos , Escala de Gravidade do Ferimento , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Picratos/sangue , Traumatismos da Medula Espinal/fisiopatologia , Fatores de TempoRESUMO
The aim of the paper was to study the effect of ascorbic acid (vitamin C) and ethanol on the level of thiobarbituric acid reactive substances (TBARS) in the brain, liver, lungs and blood serum of inbred albino BALB/c mice. Sixty male mice divided into 4 groups were used for the study; the mice in each group were injected intraperitoneally (i.p.): Group 1--control, saline; Group 2--H2O2 and FeSO4; Group 3--ascorbic acid (AA); Group 4--AA and ethanol. The level of TBARS in the investigated tissues of the mice in all of the groups was statistically significant higher than in the control group. The highest content of TBARS in blood serum, brain, liver and lung tissue was observed in mice which were given ascorbic acid and ethanol.
