RESUMO
This study was performed to evaluate the nutritional value of silkworm pupae (Bombyx mori) and the content of α-glucosidase inhibitor. The percentages of total protein and lipid contents by dry weight were 55.6 and 32.2%, respectively. Silkworm pupae protein had high levels of essential amino acids such as valine, methionine and phenylalanine. The contents of essential amino acids in silkworm pupae protein satisfied the FAO/WHO/UNU suggested requirements (2007). In addition, they also possessed n-3 fatty acids, especially α-linolenic acid (36.3%), as a major component. The 50% ethanol extract of silkworm pupae contained 1-deoxynojirimycin (DNJ), which is a potent α-glucosidase inhibitor. These results suggest that silkworm pupae are a new source of high quality protein, lipid, and α-glucosidase inhibitor.
Assuntos
1-Desoxinojirimicina/análise , Produtos Biológicos/química , Bombyx/química , Gorduras na Dieta/análise , Proteínas Alimentares/análise , Inibidores de Glicosídeo Hidrolases , Pupa/química , Aminoácidos Essenciais/análise , Animais , Ácidos Graxos Ômega-3/análise , Necessidades Nutricionais , Valor NutritivoRESUMO
Fresh cheese was prepared from caprine milk by isoelectric precipitation as a model experiment for reducing the allergenicity of milk. After acidic precipitation of casein, the beta-lactoglobulin content in curd was determined by ELISA using monoclonal antibody (MAb-beta209). The beta-lactoglobulin content was very high in the fresh cheese obtained from heat-treated (85 degrees C) bovine or caprine milk, while that obtained from untreated milk contained none of this protein. Taking it into account that caprine milk has only a small amount of alpha(s1)-casein, one of the major bovine milk allergens, the caprine fresh cheese sterilized after processing by precipitation may be useful as a protein source of low allergenicity.
Assuntos
Queijo , Manipulação de Alimentos/métodos , Cabras , Hipersensibilidade a Leite/prevenção & controle , Leite/imunologia , Animais , Anticorpos Monoclonais , Especificidade de Anticorpos , Caseínas/análise , Caseínas/imunologia , Bovinos , Queijo/análise , Precipitação Química , Ensaio de Imunoadsorção Enzimática , Temperatura Alta , Lactoglobulinas/análise , Leite/químicaRESUMO
The fatty acid composition, the lipid digestibility and protein profile of Japanese-Saanen goat's milk were characterized. Caprine milk contained substantial quantities of C(4:0) to C(10:0) fatty acids as compared with Holstein cow's milk. The lipids of the former showed significantly higher digestibility in vitro by porcine lipase than those of the latter (P<0.05). As determined by SDS-PAGE, the respective contents of alpha(s1)-casein, one of the major allergens, were 3.9% and 33.7% in caprine and bovine milk.
