Do Not Lose Your Head Over the Unequal Regeneration Capacity in Prolecithophoran Flatworms.

One of the central questions in studying the evolution of regeneration in flatworms remains whether the ancestral flatworm was able to regenerate all body parts, including the head. If so, this ability was subsequently lost in most existent flatworms. The alternative hypothesis is that head regeneration has evolved within flatworms, possibly several times independently. In the well-studied flatworm taxon Tricladida (planarians), most species are able to regenerate a head. Little is known about the regeneration capacity of the closest relatives of Tricladida: Fecampiida and Prolecithophora. Here, we analysed the regeneration capacity of three prolecithophoran families: Pseudostomidae, Plagiostomidae, and Protomonotresidae. The regeneration capacity of prolecithophorans varies considerably between families, which is likely related to the remaining body size of the regenerates. While all studied prolecithophoran species were able to regenerate a tail-shaped posterior end, only some Pseudostomidae could regenerate a part of the pharynx and pharynx pouch. Some Plagiostomidae could regenerate a head including the brain and eyes, provided the roots of the brain were present. The broad spectrum of regeneration capacity in Prolecithophora suggests that head regeneration capacity is not an apomorphy of Adiaphanida.

Sticking Together an Updated Model for Temporary Adhesion.

Non-parasitic flatworms are known to temporarily attach to the substrate by secreting a multicomponent bioadhesive to counteract water movements. However, to date, only species of two higher-level flatworm taxa (Macrostomorpha and Proseriata) have been investigated for their adhesive proteins. Remarkably, the surface-binding protein is not conserved between flatworm taxa. In this study, we sequenced and assembled a draft genome, as well as a transcriptome, and generated a tail-specific positional RNA sequencing dataset of the polyclad Theama mediterranea. This led to the identification of 15 candidate genes potentially involved in temporary adhesion. Using in situ hybridisation and RNA interference, we determined their expression and function. Of these 15 genes, 4 are homologues of adhesion-related genes found in other flatworms. With this work, we provide two novel key components on the flatworm temporary adhesion system. First, we identified a Kringle-domain-containing protein (Tmed-krg1), which was expressed exclusively in the anchor cell. This in silico predicted membrane-bound Tmed-krg1 could potentially bind to the cohesive protein, and a knockdown led to a non-adhesive phenotype. Secondly, a secreted tyrosinase (Tmed-tyr1) was identified, which might crosslink the adhesive proteins. Overall, our findings will contribute to the future development of reversible synthetic glues with desirable properties for medical and industrial applications.