Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 34
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Russo | MEDLINE | ID: mdl-15188566

RESUMO

The results of the correction of disturbances in intestinal microflora in elderly patients with the use of bifidumbacterin in the form of powder or suppositories are presented. The effectiveness of the rectal administration of bifidumbacterin (by microinjections through an enema or in suppositories) is shown in comparison with the oral administration of the preparation. The administration of bifidumbacterin in suppositories for 15 days resulted in decreased content of hemolytic Escherichia below the threshold of determination in all examined patients.


Assuntos
Anti-Infecciosos/uso terapêutico , Produtos Biológicos/uso terapêutico , Enteropatias/terapia , Administração Oral , Administração Retal , Idoso , Anti-Infecciosos/administração & dosagem , Produtos Biológicos/administração & dosagem , Contagem de Colônia Microbiana , Enema , Escherichia coli O157/isolamento & purificação , Feminino , Humanos , Enteropatias/microbiologia , Masculino , Pessoa de Meia-Idade , Supositórios/administração & dosagem
2.
Bull Exp Biol Med ; 133(2): 199-201, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12428295

RESUMO

A method for measuring urease activity in biopsy specimens and Helicobacter pylori cultures from these specimens is proposed. The method is based on measurement (with a portable pH-meter) of the rate of pH changes in a reaction mixture consisting of buffer, substrate (urea), and biopsy specimen or bacterial cells. This method revealed that urease activity of biopsy specimens correlated with that of H. pylori suspension in the same experiment. High urease activity was found in biopsy specimens containing the greatest number of Helicobacter cells; only one of 14 specimens free of H. pylori cells showed no urease activity. Introduction of this method into clinical practice will help to evaluate the contribution of H. pylori to the pathological process.


Assuntos
Mucosa Gástrica/enzimologia , Helicobacter pylori/enzimologia , Urease/metabolismo , Adulto , Idoso , Biópsia , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade
3.
Artigo em Russo | MEDLINE | ID: mdl-10852056

RESUMO

The bacteriological analysis of 302 children has revealed that dysbacteriosis with the increased content of hemolytic Escherichia in the intestine develops more frequently in the presence of the deficiency of bifido- and lactobacteria. The development of other kinds of dysbacteriosis with the increased content of different opportunistic enterobacteria, staphylococci, enterococci and fungi, as well as dysbacteriosis with the decreased content of Escherichia, does not practically depend on the deficiency of bifido- and lactobacteria. In patients with the increased content of Escherichia an increase in the content of opportunistic enterobacteria, staphylococci and fungi is observed more frequently than in patients with the low content of Escherichia.


Assuntos
Infecções Bacterianas/microbiologia , Enteropatias/microbiologia , População Urbana , Adulto , Bactérias/isolamento & purificação , Criança , Contagem de Colônia Microbiana , Fezes/microbiologia , Humanos , Moscou
5.
Artigo em Russo | MEDLINE | ID: mdl-7539974

RESUMO

The antigen-specific activity of polycomponent vaccine VP-4 consisting of Klebsiella pneumoniae, Staphylococcus aureus, Proteus vulgaris and Escherichia coli antigens was studied. The same form of the vaccine was used for both subcutaneous injection and oral administration. The oral administration of the preparation to rabbits and guinea pigs induced a rise in the level of antibodies to all components of the vaccine in their blood sera, the optimum oral doses exceed only 2- to 4-fold the doses used for subcutaneous injection. The form of vaccine VP-4, developed for subcutaneous injection, may be recommended for wider trials with its oral administration.


Assuntos
Antígenos de Bactérias/administração & dosagem , Vacinas Bacterianas/administração & dosagem , Epitopos/imunologia , Escherichia coli/imunologia , Klebsiella pneumoniae/imunologia , Proteus vulgaris/imunologia , Staphylococcus aureus/imunologia , Administração Oral , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Cobaias , Imunização/métodos , Injeções Subcutâneas , Coelhos , Vacinas Combinadas
6.
Radiats Biol Radioecol ; 34(4-5): 582-6, 1994.
Artigo em Russo | MEDLINE | ID: mdl-7951888

RESUMO

It was shown in experiments with mice and dogs that bacterial preparations (vaccinum proteus, prodigiosanum, bificolum and bificolum-f) administered before or after gamma-irradiation have a stimulating effect on hemopoiesis. It is supposed that bacterial polysaccharides play an important role in this action.


Assuntos
Produtos Biológicos/uso terapêutico , Hematopoese/efeitos dos fármacos , Lesões Experimentais por Radiação/terapia , Doença Aguda , Animais , Vacinas Bacterianas/uso terapêutico , Bacteriocinas/uso terapêutico , Cães , Relação Dose-Resposta à Radiação , Camundongos , Prodigiozan/uso terapêutico , Proteus/imunologia , Lesões Experimentais por Radiação/sangue , Fatores de Tempo
9.
Artigo em Russo | MEDLINE | ID: mdl-8067073

RESUMO

Multicomponent vaccine VP-4, prepared from Streptococcus, Klebsiella, Proteus and Escherichia coli antigens, was tested for protective activity by oral administration to mice with experimental Klebsiella, Proteus and S. pneumoniae infections. Vaccine VP-4, when introduced orally (in 2-3 administrations of 2 mg), was found to produce a protective effect with respect to K. pneumoniae (the effectiveness index was equal to 5.7 and 4.3). With respect to P. vulgaris, the effectiveness index was 2.7 (immunization was made in two oral administrations of 2 mg); in pneumococcal infection preliminary oral immunization with vaccine VP-4 in two administrations in a dose of 2-4 mg produced high protective effect. Thus, immunization with vaccine VP-4, introduced orally in comparatively small doses, proved to be effective in animal models used in the experiment.


Assuntos
Vacinas Bacterianas/imunologia , Escherichia coli/imunologia , Klebsiella pneumoniae/imunologia , Proteus vulgaris/imunologia , Staphylococcus aureus/imunologia , Vacinas Combinadas/imunologia , Administração Oral , Animais , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/toxicidade , Relação Dose-Resposta Imunológica , Avaliação Pré-Clínica de Medicamentos , Imunização , Infecções por Klebsiella/prevenção & controle , Dose Letal Mediana , Camundongos , Infecções Pneumocócicas/prevenção & controle , Infecções por Proteus/prevenção & controle , Vacinas Combinadas/administração & dosagem , Vacinas Combinadas/toxicidade
10.
Artigo em Russo | MEDLINE | ID: mdl-1441812

RESUMO

The results of the study of the antigenic activity of multicomponent vaccine consisting of staphylococcal, Klebsiella, Proteus, Escherichia coli antigens and staphylococcal monovaccine, introduced into rabbits and guinea pigs by different routes, are presented. As shown in this study, the multicomponent vaccine introduced orally in 5 administrations stimulated the production of antibodies to all components, but the intensity of antibody formation to each of them was different. Antibodies to E. coli antigen were found to be at the lowest level. Staphylococcal antigen, introduced orally both as the component of the multicomponent vaccine and as the monovaccine, ensured pronounced stimulation of the production of antistaphylococcal antibodies, not lower than that observed after subcutaneous injection. The comparative study of the activity of the multicomponent vaccine introduced by combined routes, oral-subcutaneous and subcutaneous-oral, showed the advantage of the former one.


Assuntos
Antígenos de Bactérias/administração & dosagem , Vacinas Bacterianas/administração & dosagem , Imunização/métodos , Administração Oral , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Combinação de Medicamentos , Escherichia coli/imunologia , Cobaias , Injeções Subcutâneas , Klebsiella pneumoniae/imunologia , Proteus vulgaris/imunologia , Coelhos , Vacinas Antiestafilocócicas/administração & dosagem , Vacinas Antiestafilocócicas/imunologia , Staphylococcus aureus/imunologia , Fatores de Tempo
11.
Zh Mikrobiol Epidemiol Immunobiol ; (4): 39-41, 1991 Apr.
Artigo em Russo | MEDLINE | ID: mdl-1882605

RESUMO

Multicomponent vaccine prepared from the antigens of 4 representatives of opportunistic microflora possesses high specific activity. The passive hemagglutination (PHA) test with the use of associated diagnosticum showed that antibody titers in the sera of immunized rabbits increased 10- to 10(4)-fold in comparison with the titers observed prior to immunization. The PHA test with the use of the antigens contained in the vaccine revealed the accumulation of antibodies to each of the 4 components of the preparation in the blood sera of immunized rabbits. When stored at 4 degrees C, the vaccine was shown to retain its specific activity for 5 years (the term of observation).


Assuntos
Anticorpos Antibacterianos/sangue , Especificidade de Anticorpos/imunologia , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Escherichia coli/imunologia , Klebsiella pneumoniae/imunologia , Proteus vulgaris/imunologia , Staphylococcus aureus/imunologia , Animais , Testes de Hemaglutinação , Imunização , Coelhos , Fatores de Tempo
12.
Klin Med (Mosk) ; 68(11): 75-7, 1990 Nov.
Artigo em Russo | MEDLINE | ID: mdl-2283824

RESUMO

As early as the first 24 hours since a severe isolated locomotor trauma the patients were immunized with Proteus vaccine. Such vaccination is shown to stimulate the production of serum specific antibodies, to reduce contamination of the wound with gram-negative agents, to shorten hospital stay and healing of the wound versus subjects vaccinated with staphylococcal anatoxin and nonimmunized patients.


Assuntos
Vacinas Bacterianas/administração & dosagem , Proteus/imunologia , Ferimentos e Lesões/terapia , Vacinas Bacterianas/imunologia , Serviço Hospitalar de Emergência , Estudos de Avaliação como Assunto , Feminino , Humanos , Imunização , Masculino , Moscou , Vacinas Antiestafilocócicas/administração & dosagem , Infecção dos Ferimentos/imunologia , Infecção dos Ferimentos/prevenção & controle , Ferimentos e Lesões/imunologia
13.
Artigo em Russo | MEDLINE | ID: mdl-2201154

RESUMO

Klebsiella vaccine, when injected subcutaneously to donors, proved to be faintly reactogenic and safe. The injection of the vaccine had no effect on changes in the morphological composition of peripheral blood and on liver function. In persons with the initially low content of IgG an increase in this characteristic was observed after immunization. No changes in the synthesis of IgE occurred in healthy donors under the influence of immunization. The vaccine was shown to be immunogenic when introduced according to immunization schedules comprising 3 and 5 injections, the titer of Klebsiella antibodies increasing 3- to 5-fold.


Assuntos
Vacinas Bacterianas/efeitos adversos , Doadores de Sangue , Klebsiella pneumoniae/imunologia , Adulto , Anticorpos Antibacterianos/sangue , Vacinas Bacterianas/imunologia , Avaliação de Medicamentos , Feminino , Humanos , Esquemas de Imunização , Imunoglobulinas/análise , Injeções Subcutâneas , Masculino , Pessoa de Meia-Idade , Fatores de Tempo
14.
Zh Mikrobiol Epidemiol Immunobiol ; (11): 23-6, 1989 Nov.
Artigo em Russo | MEDLINE | ID: mdl-2694690

RESUMO

The comparative study of the chemical composition and biological properties of antigens isolated from Proteus vulgaris with the use of hydroxylamine and by two classical methods (Boivin's and Westphal's methods) has been made. As shown in this study, the treatment of bacteria with hydroxylamine makes it possible to obtain antigenic complexes with lower toxicity. At the same time hydroxylamine produces no denaturing effect on lipopolysaccharides and protein fractions of bacterial cells.


Assuntos
Antígenos de Bactérias/análise , Hidroxilaminas , Proteus vulgaris/imunologia , Animais , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/isolamento & purificação , Antígenos de Bactérias/toxicidade , Fenômenos Químicos , Química , Hidroxilamina , Imunização , Técnicas Imunológicas , Indicadores e Reagentes , Dose Letal Mediana , Lipopolissacarídeos/análise , Lipopolissacarídeos/imunologia , Lipopolissacarídeos/isolamento & purificação , Lipopolissacarídeos/toxicidade , Camundongos , Proteus vulgaris/efeitos dos fármacos
15.
Zh Mikrobiol Epidemiol Immunobiol ; (10): 54-9, 1989 Oct.
Artigo em Russo | MEDLINE | ID: mdl-2692352

RESUMO

The data on the study of the reactogenicity, safety and prophylactic potency of a new acellular vaccine prepared from S. flexneri 2a antigenic complexes are presented. According to the results of two epidemic experiments, the vaccine, introduced by oral administration, showed low reactogenicity, safety and sufficient prophylactic potency. The vaccine decreased morbidity rate in dysentery caused by S. flexneri 2a and ensured the protection of 74% (72-80%) of the vaccinees. The complete course of immunization consisting of three administrations followed by the booster administration induces the formation of specific immunity whose duration is sufficient for ensuring the protection of immunized persons during the epidemiologically unfavorable period (for at least 3 months).


Assuntos
Vacinas Bacterianas/administração & dosagem , Shigella flexneri/imunologia , Administração Oral , Adolescente , Adulto , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/efeitos adversos , Vacinas Bacterianas/imunologia , Relação Dose-Resposta Imunológica , Avaliação de Medicamentos , Disenteria Bacilar/epidemiologia , Disenteria Bacilar/prevenção & controle , Humanos , Masculino , Comprimidos , U.R.S.S./epidemiologia
17.
Zh Mikrobiol Epidemiol Immunobiol ; (5): 16-20, 1989 May.
Artigo em Russo | MEDLINE | ID: mdl-2476896

RESUMO

Highly sensitive and specific erythrocyte diagnostic agents (ED) for the determination of antibodies to Proteus O-antigens have been obtained by the sensitization of formolated sheep red blood cells (SPBC) with activated lipopolysaccharides (LPS) without the use of mediators. The tannin treatment of formolated SRBC and/or the increase of temperature from 45 degrees C to 100 degrees C in the process of the preparation of ED have been found to produce no increase in effectiveness. Antibody ED permitting the detection of Proteus O- and H-antigens has been obtained by the sensitization of formolated chick red blood cells with immunoglobulin preparations to Proteus hydroxylamine antigens, carried out with the use of amidol. The experiments have shown the possibility of using this antibody ED for the determination of O-antibodies in the antigen neutralization test with nonactivated LPS used as an agglutinating agent. The passive hemagglutination test with antibody ED has proved to be a more sensitive method for the detection of O-antibodies than the antigen neutralization test with antigenic ED. The determination of Proteus etiology in the passive hemagglutination test with the use of antigenic ED has been shown to be highly effective in the examination of patients with chronic osteomyelitis at the stage of exacerbation.


Assuntos
Anticorpos Antibacterianos/análise , Antígenos de Bactérias/imunologia , Eritrócitos/imunologia , Proteus/imunologia , Animais , Especificidade de Anticorpos , Doença Crônica , Testes de Hemaglutinação , Humanos , Soros Imunes/imunologia , Soros Imunes/isolamento & purificação , Imunização , Testes de Neutralização , Antígenos O , Osteomielite/imunologia , Coelhos
18.
Artigo em Russo | MEDLINE | ID: mdl-3140544

RESUMO

S. aureus aqueous extract and K. pneumoniae hydroxylamine vaccine were studied by means of chemical and immunochemical analytical techniques. The preparations were found to contain, respectively, 7.0% nad 53.5% of neutral monosaccharides, 6.5% and 0.7% of nucleic acids, as well as protein in approximately equal amounts (11.63-14.0%). In experiment of immunodiffusion, immunoelectrophoresis and rocket immunoelectrophoresis in homologous systems with hyperimmune antimicrobial sera the preparations were characterized by serological heterogeneity. After their combination with Escherichia coli aqueous extract and Proteus hydroxylamine preparation their serological characteristics remaIned unchanged. The study of cross reactions of all components of the combined preparations with hyperimmune rabbit sera to the corresponding microorganisms revealed that only Klebsiella component of the combined vaccine reacted with all hyperimmune sera. The preparation of Proteus showed the lowest activity, it reacted only with hyperimmune sera to K. pneumoniae. Besides, no reaction of S. aureus component with sera to E. coli and no reaction of the preparation of E. coli with antistaphylococcal serum were observed.


Assuntos
Antígenos de Bactérias/análise , Vacinas Bacterianas/análise , Klebsiella pneumoniae/imunologia , Staphylococcus aureus/imunologia , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Fenômenos Químicos , Química , Reações Cruzadas , Combinação de Medicamentos , Escherichia coli/imunologia , Soros Imunes/imunologia , Imunoquímica , Imunodifusão , Imunoeletroforese , Proteus vulgaris/imunologia
19.
Zh Mikrobiol Epidemiol Immunobiol ; (1): 58-61, 1988 Jan.
Artigo em Russo | MEDLINE | ID: mdl-2452532

RESUMO

The results of the study of rabbit antisera to meningococci A, B, C in the double diffusion in gel, passive hemagglutination test and enzyme immunoassay with antigenic preparations isolated from S. aureus strains are indicative of the presence of common antigenic determinants of protein and polysaccharide nature in S. aureus and N. meningitidis.


Assuntos
Anticorpos Antibacterianos/análise , Antígenos de Bactérias/análise , Neisseria meningitidis/imunologia , Staphylococcus aureus/imunologia , Animais , Especificidade de Anticorpos , Reações Cruzadas , Epitopos/análise , Testes de Hemaglutinação , Imunodifusão , Técnicas Imunoenzimáticas , Neisseria meningitidis/classificação , Coelhos , Sorotipagem
20.
Zh Mikrobiol Epidemiol Immunobiol ; (12): 57-61, 1987 Dec.
Artigo em Russo | MEDLINE | ID: mdl-3128030

RESUMO

The comparative study of the immunological activity of Proteus vaccine prepared from soluble antigenic complexes was made after the immunization of volunteers with this vaccine used in the form of a single preparation and in combination with pyoimmunogen (Pseudomonas aeruginosa vaccine) and/or adsorbed staphylococcal toxoid. The injection of the vaccine in the form of a single preparation and in different combinations increased the ingestion of Proteus cells by neutrophils. The injection of Proteus vaccine simultaneously with pyoimmunogen and staphylococcal toxoid ensured the intensive phagocytosis of staphylococci. All combinations with Proteus vaccine, used in this investigation, stimulated the intensive formation of antibodies to Proteus vaccine strain and Re-glycolipid. Proteus vaccine introduced in combination with adsorbed staphylococcal toxoid essentially stimulated the synthesis of anti-alpha-staphylolysin.


Assuntos
Vacinas Bacterianas/imunologia , Proteus vulgaris/imunologia , Pseudomonas aeruginosa/imunologia , Toxoide Estafilocócico/imunologia , Adolescente , Adsorção , Adulto , Antígenos de Bactérias/imunologia , Endotoxinas/imunologia , Escherichia coli/imunologia , Glicolipídeos/imunologia , Hemaglutininas/análise , Humanos , Neutrófilos/imunologia , Fagocitose , Vacinas contra Pseudomonas , Staphylococcus/imunologia , Fatores de Tempo , Vacinas Combinadas
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...