Umbilical diagnosis as a non-invasive visual diagnostic aide.

Using Bi-Digital O-Ring Test (Omura, Y. 1977-2002; BDORT), we have researched the abnormal shapes of the umbilicus and the surrounding areas of the umbilicus, and the relationship between diseased internal organs and abnormal shape and direction of the radial sulcus of the umbilicus for several years. In this report we have mapped the organ representation area of the umbilicus and its surrounding areas of a healthy person by using BDORT. Then the abnormal organs around the umbilicus of the patients with certain diseases were mapped by using BDORT. Also Oncogene C-fos Ab2 positive response network (specially relation between the radial sulcus and the disease) of the cancer response (Oncogene C-fos Ab2) positive person were examined all over the body. In many cases, the radial sulcus from the umbilicus and the organ representation area of abnormal organs at the umbilicus of diseased person mapped using BDORT were highly correlated. Abnormality observed in a specific organ with strong resonance by BDORT meridian network was connected to the umbilicus. Mapped chart of the organ representation area surrounding the umbilicus is clinically very useful for non-invasive visual umbilical diagnosis.

Relationship of p21 (waf1/cip1) and differentiation in chondrosarcoma cells.

The histological grade of chondrosarcoma correlates well with their clinical behavior and with the patient's survival duration. We have previously demonstrated that p21 was expressed in the hypertrophic chondrocytes of the growth plate. To assess the relationship of p21 (waf1/cip1) to cell differentiation in chondrosarcoma, we examined the p21 expression in 14 cases of chondrosarcoma immunohistochemically and the induction of p21 by insulin-like growth factor-I (IGF-I) during cell differentiation in SW1353 chondrosarcoma cells. p21 immunoreactivity was seen in well-differentiated chondrosarcoma cells and was mutually exclusive with MIB1 reactivity in grade-1 chondrosarcoma. In vitro, the proteoglycan synthesis of SW1353 cells was increased by IGF-I in a dose-dependent manner. However, cell proliferation was not markedly stimulated. Overexpressions of p21 mRNA and p21 protein in SW1353 cells were induced by IGF-I 100 ng/ml. Our results suggested that the p21 expression was directly related to tumor differentiation and that the p21 expression was an important mediator for IGF-I in chondrosarcoma cells.