RESUMO
This study aims to investigate the effect of Polygonum odoratum leaf extract (POE) on oxidative stress markers and cell death induced by low dose ionizing radiation (LDIR) in Raw 264.7 cells. The biological activities, chromatographic fingerprint, and cytotoxicity of POE were investigated. To determine the radioprotective effect of POE, Raw 264.7 cells were incubated with POE for 1 hr prior to 100 mGy x-irradiation. The cell viability, oxidative stress damage marker (malondialdehyde level; MDA), and endogenous antioxidant markers (superoxide dismutase: SOD, catalase: CAT, and glutathione peroxidase: GSH-Px) were also determined. The results showed that POE contained 8 essential substances and exhibited a potent antioxidant without any cytotoxicity. It was found that POE significantly decreased the MDA level and activated cell viability, SOD, CAT, and GSH-Px activities. The results from this study indicate that POE is a potent antioxidant, which can be developed as a radioprotector for diagnostic procedures. PRACTICAL APPLICATIONS: Polygonum odoratum leaf extract (POE) is a potent antioxidant that attenuates oxidative stress and cell death induced by low dose ionizing radiation (LDIR). POE might protect against cell damage from LDIR, particularly in diagnostic radiology procedures. Therefore, the development of functional food containing POE might be beneficial for patients who plan to undergo the diagnostic radiology procedure. The functional food containing POE might prevent stochastic and deterministic effects for these patients.
Assuntos
Antioxidantes , Estresse Oxidativo , Extratos Vegetais , Polygonum , Animais , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Morte Celular , Camundongos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polygonum/química , Células RAW 264.7 , Radiação Ionizante , Superóxido Dismutase/metabolismoRESUMO
Currently, the therapeutic strategy against metabolic syndrome and its complications is required due to the increasing prevalence and its impact. Due to the benefits of both mulberry fruit extract and encapsulation technology, we hypothesized that encapsulated mulberry fruit extract (MME) could improve metabolic parameters and its complication risk in postmenopausal metabolic syndrome. To test this hypothesis, female Wistar rats were induced experimental menopause with metabolic syndrome by bilateral ovariectomy (OVX) and high-carbohydrate high-fat (HCHF) diet. Then, they were orally given MME at doses of 10, 50, and 250 mg/kg BW for 8 weeks and the parameters, such as percentage of body weight gain, total cholesterol, triglycerides, HDL-C, LDL-C, atherogenic index, fasting blood glucose, plasma glucose area under the curve, serum angiotensin-converting enzyme (ACE), oxidative stress status, histology, and protein expression of PPAR-γ, TNF-α, and NF-κB in adipose tissues were determined. MME improved body weight gain, adiposity index, glucose intolerance, lipid profiles, atherogenic index, ACE, oxidative stress status, and protein expression of TNF-α and NF-κB. Moreover, MME attenuated adipocyte hypertrophy and enhanced PPAR-γ expression. Taken altogether, MME decreased metabolic syndrome and its complication via the increased PPAR-γ expression. Therefore, MME is the potential candidate for improving metabolic syndrome and its related complications. However, further research in clinical trial is still necessary.
Assuntos
Frutas/química , Menopausa/efeitos dos fármacos , Síndrome Metabólica/tratamento farmacológico , Morus/química , Extratos Vegetais/uso terapêutico , Animais , Dieta Hiperlipídica , Feminino , Síndrome Metabólica/sangue , Síndrome Metabólica/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo/efeitos dos fármacos , PPAR gama/metabolismo , Peptidil Dipeptidase A/sangue , Ratos Wistar , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Currently, the neuroprotectant and memory-enhancing agent for menopausal women with metabolic syndrome is required. Based on the advantages of polyphenolics on numerous changes observed in menopause with metabolic syndrome and the encapsulation method, we hypothesized that microencapsulated mulberry fruit extract (MME) could protect brain damage and improve memory impairment in an animal model of menopause with metabolic syndrome. To test this hypothesis, MME at doses of 10, 50, and 250 mg/kg was given to female Wistar rats which were induced experimental menopause with metabolic syndrome by bilateral ovariectomy (OVX) and fed with high-carbohydrate high-fat (HCHF) diet for 8 weeks. Spatial memory together with neuron density, oxidative stress status, acetylcholinesterase, and phosphorylation of Erk in the hippocampus was assessed at the end of the study. It was found that MME decreased memory impairment, oxidative stress status, and AChE activity but increased neuron density and Erk phosphorylation in the hippocampus. Therefore, the neuroprotective and memory-enhancing effects of MME might partly involve the enhanced cholinergic function and Erk phosphorylation but decreased oxidative stress status in hippocampus. Therefore, MME is the potential novel neuroprotectant and memory-enhancing agent for menopause with metabolic syndrome. However, further research especially clinical trial is still necessary.