RESUMO
Lymph dilution was used in 26 females with inflammation of the uterine appendages and 22 patients with appendicular infiltrate. 500 ml of sodium chloride solution with gentamycin (1.4 mg/kg) were dripped in the subcutaneous fat of the femur anterior surface. Pneumocompression was performed on the unit APKU-5. The response was identical to the direct endolymphatic antibiotic therapy.
Assuntos
Antibacterianos/administração & dosagem , Apendicite/tratamento farmacológico , Endotoxinas , Gentamicinas/administração & dosagem , Linfa , Doença Inflamatória Pélvica/tratamento farmacológico , Animais , Cães , Feminino , Humanos , Masculino , Modelos TeóricosRESUMO
We have utilized primary cultures of free-floating clusters obtained from pancreata of human cadaveric fetuses. Clusters have been incubated for 10 days in RPMI-1640 medium with 3H-thymidine and various concentrations of fetal calf serum and glucose. Viability of clusters was assessed by insulin radioimmunoassay. Cell reproduction was investigated by radioautography. We have revealed 2 populations of proliferating epithelial cells in clusters: the minor population of cells of duct-like structures and the major population of other epithelial cells. Rate of accumulation of labelled cells in both populations in clusters of 17-18-week-old fetuses was significantly higher than in clusters of 23-24-week-old fetuses. Proliferation of cells of duct-like structures was regulated by serum but not by glucose, whereas proliferation of other epithelial cells depended on both serum and glucose concentrations.