[Approaches for formation of the large bowel anastomoses for prophylaxis of their sutures insufficiency].

The results of surgical treatment of 424 patients, in whom the postponed and elective surgery was done for the large bowel malignancies, were analyzed. Advantages of a one-layer suture while formation of a large-bowel anastomoses in comparison with a two-layer suture were noted: minimal rate of the sutures insufficiency--(4.9 +/- 0.8)%, and (7.9 +/- 1.7)%, accordingly (p<0.001). Mostly often insufficience of the colorectal anastomosis sutures was observed after anterior rectal resection--in (9.7 +/- 1.9)% and (7.2 +/- 1.5)% patients. Postoperative lethality was 2.6%, for majority of the patients the reasons of the death were pulmonary thromboembolism and an acute cardio-vascu- lar insufficiency.

[Exploration and identification of Physcomitrella patens moss peptides].

In the current study the isolation and identification of Physcomitrella patens (Hedw.) B.S.G. moss peptides are described. Physcomitrella patens moss is actively used in recent years as a model organism to study the biology of plants. Protoplasts, protonemata and gametophores of the moss are demonstrated for the first time to contain diverse small peptides. From gametophores was isolated and identified 58 peptides that are fragments of 14 proteins, and from protonemata - 49 peptides, fragments of 15 proteins. It was found that the protonemata and gametophores Ph. patens, which are the successive stages of development of this plant, significantly different from each other as a peptide composition and the spectrum of the precursor protein of identified peptides. Isolation of protoplasts of the enzymatic destruction of cell wall protonemata accompanied by massive degradation of intracellular proteins, many of whom are proteins of photosynthesis, which is a characteristic response of plants to stress the impact of environmental factors. A total of moss protoplasts were isolated and identified 323 peptides that are fragments of 79 proteins.

Proteome analysis of chloroplasts from the moss Physcomitrella patens (Hedw.) B.S.G.

Intact chloroplasts were prepared from protoplasts of the moss Physcomitrella patens according to an especially developed method. They were additionally separated into stroma and thylakoid fractions. The proteomes of intact plastids, stroma, and thylakoids were analyzed by 1D-electrophoresis under denaturing conditions followed by protein digestion and nano-LC-ESI-MS/MS of tryptic peptides from gel bands. A total of 624 unique proteins were identified, 434 of which were annotated as chloroplast resident proteins. The majority of proteins belonged to a photosynthetic group (21.3%) and to the group of proteins implicated in protein degradation, posttranslational modification, folding, and import (20.6%). Among proteins assigned to chloroplasts, the following groups are prominent combining proteins implicated in metabolism of: amino acids (6.9%), nucleotides (2.5%), lipids (2.2%), carbohydrates (2.4%), hormones (1.5%), isoprenoids (1.25%), vitamins and cofactors (1%), sulfur (1.25%), and nitrogen (1%); as well as proteins involved in the pentose-phosphate cycle (1.75%), tetrapyrrole synthesis (3.7%), and redox processes (3.6%). The data can be used in physiological and photobiological studies as well as in further studies of P. patens chloroplast proteome including structural and functional specifics of plant protein localization in organelles.

[Initial manifestations of alcoholism in adolescents (social-psychological and clinical aspects)]. / Osobennosti nachal'nykh proiavlenii alkogolizma u podrostkov (sotsial'no-psikhologicheskii i klinicheskii aspekty).

The authors studied the traits of initial symptoms of early alcoholism in 100 boys-pupils of a special boarding-school. It was established that in the appearance of early alcoholism a certain role is being played by unhappy families. A significant importance in the formation of an inclination for alcohol is the character of the first pharmacological influence: euphoric reaction of the first intoxication increases the risk of becoming an alcoholic. The most expressed initial signs are the following: a stable pathological craving for alcohol, use of substitutes, transformation of intoxication.