RESUMO
We studied the neutrophils and monocytes obtained from 37 patients with various inflammatory diseases such as psoriasis, acute infectious process in the abdominal cavity (acute appendicitis/abscess of the abdominal cavity, and acute cholecystitis), acute pancreatitis, and post-COVID syndrome after mild COVID infection. The number and the morphological structure of neutrophil extracellular traps (NET) as well as the effect of IgG on NET were examined. NET were visualized and counted by fluorescence microscopy with fluorescent dye SYBR Green. All the studied types of inflammation were accompanied by spontaneous formation of NET. After application of IgG, the number of NET doubled, their size increased, and transformation of net-like traps into the cloud forms was observed. The clouds form structure of the network is not capable of capturing pathogens with subsequent retraction, the products of its enzymatic degradation can be the factors of secondary alteration. The study results demonstrate a previously unknown mechanism of infection resistance.
Assuntos
COVID-19 , Armadilhas Extracelulares , Pancreatite , Humanos , Armadilhas Extracelulares/metabolismo , Doença Aguda , Pancreatite/metabolismo , COVID-19/metabolismo , Neutrófilos/metabolismo , Imunoglobulina G/metabolismoRESUMO
Tissue damage in various diseases, hypoxic conditions, and some pathologies are associated with production of endogenous factors such as the soluble form of the surface receptor CD100, malonic dialdehyde, and arginase and their release into circulation. These factors modulate functional state of lymphocytes in the immune system: potentiate activation of B lymphocytes, activate synthesis and secretion of IL-25 and IL-17 cytokines, and suppress proliferative activity of T lymphocytes, thus modulating immunological reactivity of the organism. Reactions of innate and adaptive immunity develop against the background of changed immunological reactivity, which should be taken into account in the development of pathogenetically substantiated therapy.
Assuntos
Antígenos CD/metabolismo , Arginase/metabolismo , Semaforinas/metabolismo , Animais , Antígenos CD/genética , Arginase/genética , Linfócitos B/imunologia , Linfócitos B/metabolismo , Humanos , Interleucina-17/metabolismo , Semaforinas/genética , Linfócitos T/metabolismoRESUMO
The influence of regulatory peptide type of AFP on the course of experimental ulcer was investigated. It has been shown that activation of apoptosis enhances local necrotic inflammatory reaction, on the one hand, and enables the development of adhesions with on the other.
Assuntos
Apoptose/efeitos dos fármacos , Modelos Animais de Doenças , Encefalinas/farmacologia , Linfócitos/efeitos dos fármacos , Complexo Mioelétrico Migratório/efeitos dos fármacos , Fragmentos de Peptídeos/farmacologia , Úlcera Gástrica/patologia , Animais , Encefalinas/administração & dosagem , Linfócitos/patologia , Fragmentos de Peptídeos/administração & dosagem , Ratos Wistar , Úlcera Gástrica/fisiopatologia , Aderências Teciduais/patologia , Aderências Teciduais/fisiopatologiaRESUMO
The influence of regulatory peptide type of AFP on the course of experimental ulcers was investigated. It has been shown that activation of apoptosis by AFP enhance local necrotic inflammatory reaction, on the one hand, and enables the development of adhesions with the other.
Assuntos
Apoptose/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Fragmentos de Peptídeos/farmacologia , Úlcera Gástrica/imunologia , alfa-Fetoproteínas/farmacologia , Animais , Modelos Animais de Doenças , Humanos , Linfócitos/patologia , Complexo Mioelétrico Migratório , Ratos Wistar , Úlcera Gástrica/sangue , Úlcera Gástrica/patologia , Úlcera Gástrica/fisiopatologiaRESUMO
Latent sensitization is a risk factor of a clinically manifest atopic reaction. Investigation of a phenotypic composition of lymphocytes reveals function of the immune system in this condition. Indirect fluorescence assessed differential and activation lymphocyte antigens (CD4, CD3, CD8, CD16, CD20 and CD25, CD71, HLA-DR, CD95, CD23, CD54, respectively) in 15 patients with latent sensitization, 32 patients with atopic bronchial asthma and 22 healthy donors. The immune system demonstrated definite activation with involvement ofT- and B-links of the immune system. Lymphocyte activation in latent sensitization and bronchial asthma differs by high expression of CD95 apoptosis trigger receptor. Enhancement of Fas-mediated lymphocyte apoptosis may inhibit IgE synthesis and atopic pathology manifestation.
Assuntos
Asma/imunologia , Hipersensibilidade Tardia/imunologia , Ativação Linfocitária/imunologia , Linfócitos/imunologia , Adulto , Alérgenos/imunologia , Antígenos CD/sangue , Antígenos CD/imunologia , Asma/sangue , Estudos de Casos e Controles , Feminino , Humanos , Hipersensibilidade Tardia/sangue , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Masculino , Pessoa de Meia-Idade , Pólen/imunologia , Testes CutâneosRESUMO
The extract of the Tibetan medicine Taban-Arshan corrects changes of the immune system in inflammatory diseases--rheumatoid arthritis and atopic bronchial asthma. An immunomodulating effect of the drug consists in its ability to suppress hyperactivation of lymphocytes by normalization of expression of basic activation antigens.
Assuntos
Antígenos CD/imunologia , Artrite Reumatoide/imunologia , Asma/imunologia , Fatores Imunológicos/farmacologia , Linfócitos/efeitos dos fármacos , Medicina Tradicional Tibetana , Extratos Vegetais/farmacologia , Adulto , Antígenos CD/biossíntese , Artrite Reumatoide/sangue , Asma/sangue , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Linfócitos B/metabolismo , Humanos , Técnicas In Vitro , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Ativação Linfocitária/efeitos dos fármacos , Contagem de Linfócitos , Linfócitos/imunologia , Linfócitos/metabolismo , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Linfócitos T/metabolismoRESUMO
Taban-Arshan extract decreased expression of T-lymphocyte activation markers, normalized T-cell-mediated immunity, and suppressed increased activity of natural killer receptors during culturing with lymphocytes of patients with atopic bronchial asthma. Taban-Arshan extract normalized activation processes in the B-cell immunity and stimulated expression of receptors of activation-induced apoptosis.
Assuntos
Antiasmáticos/imunologia , Asma/imunologia , Medicina Tradicional Tibetana , Adulto , Antiasmáticos/metabolismo , Anticorpos Monoclonais/metabolismo , Antígenos CD/efeitos dos fármacos , Antígenos CD20/efeitos dos fármacos , Asma/tratamento farmacológico , Asma/metabolismo , Linfócitos B/imunologia , Complexo CD3/efeitos dos fármacos , Células Cultivadas , Técnica Direta de Fluorescência para Anticorpo , Humanos , Molécula 1 de Adesão Intercelular/efeitos dos fármacos , Células Matadoras Naturais/imunologia , Leucócitos Mononucleares/imunologia , Ativação Linfocitária , Extratos Vegetais/uso terapêutico , Receptores de IgE/efeitos dos fármacos , Receptores de IgG/efeitos dos fármacos , Receptores de Interleucina-2/efeitos dos fármacos , Linfócitos T/imunologiaAssuntos
Doenças Transmissíveis/complicações , Síndromes de Imunodeficiência/etiologia , Inflamação/complicações , Anti-Infecciosos/efeitos adversos , Anti-Infecciosos/uso terapêutico , Doenças Transmissíveis/tratamento farmacológico , Doenças Transmissíveis/imunologia , Resistência Microbiana a Medicamentos , Humanos , Síndromes de Imunodeficiência/imunologia , Inflamação/tratamento farmacológico , Inflamação/imunologiaAssuntos
Hipersensibilidade/imunologia , Miocardite/imunologia , Células Apresentadoras de Antígenos/patologia , Linfócitos B/imunologia , Linfócitos B/patologia , Biomarcadores/sangue , Progressão da Doença , Humanos , Hipersensibilidade/sangue , Hipersensibilidade/complicações , Hipersensibilidade/microbiologia , Miocardite/sangue , Miocardite/complicações , Miocardite/microbiologia , Linfócitos T/imunologia , Linfócitos T/patologiaAssuntos
Antígenos CD/imunologia , Antígenos de Superfície/imunologia , Linfócitos/efeitos dos fármacos , Neuropeptídeos/farmacologia , Ferimentos e Lesões/imunologia , Adenosina/farmacologia , Difosfato de Adenosina/análogos & derivados , Difosfato de Adenosina/farmacologia , Sequência de Aminoácidos , Feminino , Humanos , Linfócitos/imunologia , Masculino , Dados de Sequência Molecular , Ácido Pirrolidonocarboxílico/análogos & derivadosRESUMO
Morphometric analysis of the left ventricle myocardial layers under heart hypertrophy was carried out. Values of tissue parameters on the 3rd, 10th and 24th days after 50% coarctation of the abdominal aorta were received. Is shown the heart functioning under conditions of myocardial compensatory hypertrophy development is characterized by expressed zones and different directions of changes of quantitative parameters of tissue components. The single injection of HMP (Hydra morphogen peptide) made the development of myocardial hypertrophy easy at early stages, decreasing depth of manifestations morphologic changes and perhaps affected the time of their development.
Assuntos
Adaptação Fisiológica/efeitos dos fármacos , Coração/efeitos dos fármacos , Hipertrofia Ventricular Esquerda/patologia , Miocárdio/patologia , Neuropeptídeos/farmacologia , Animais , Ventrículos do Coração/efeitos dos fármacos , Ventrículos do Coração/patologia , Hipertrofia Ventricular Esquerda/etiologia , Masculino , Ácido Pirrolidonocarboxílico/análogos & derivados , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The morphological study showed that the single injection of HMP (Hydra Morphogen Peptide) facilitated the development of the myocardium hypertrophy. This effect is expressed in the subendocardial layer most of all, where the normalization of the relative volume and diameter of the cardiomyocytes and the decrease of the connective tissue oedema are noted. These parameters are normalized in the intramural and subepicardial layers in less degree. The decrease of the connective tissue oedema is noted in the intramural layer. The normal correlation recovery between the relative volumes of the cardiomyocytes and the connective tissue is shown in the subepicardial layer, the diameters of the myocytes are essentially increased. The influence of HMP is manifested not only on the muscular and connective tissue components of the myocardium, but touches on also the system of the myocardial wall vessels, causing the redistribution of the intramural blood circulation.
Assuntos
Cardiomegalia/patologia , Coração/efeitos dos fármacos , Neuropeptídeos/farmacologia , Animais , Cardiomegalia/induzido quimicamente , Masculino , Miocárdio/ultraestrutura , Ácido Pirrolidonocarboxílico/análogos & derivados , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The dependence of ornithine decarboxylase (ODC) activity on hydra peptide morphogen doses has been established. The parameters of protein synthesis were determined in normal and regenerating rat liver, using the peptide in a dose of 20 micrograms/kg body weight, initiating maximum enzyme activity. It was established that intraperitoneal injection of the peptide in partially hepatectomized animals stimulated ODS activity in dose-dependent manner and was dome-shaped. The peptide injection in intact animals does not affect the intensity of 3H-leucine inclusion into the liver protein and the protein content in rat liver. However, the peptide injection in partially hepatectomized animals increased the level of 3H-leucine inclusion into the protein of regenerating liver and stimulated protein accumulation in this type of tissue.
Assuntos
Regeneração Hepática/efeitos dos fármacos , Fígado/efeitos dos fármacos , Neuropeptídeos/farmacologia , Biossíntese de Proteínas , Animais , Injeções Intraperitoneais , Fígado/metabolismo , Masculino , Neuropeptídeos/administração & dosagem , Ornitina Descarboxilase/metabolismo , Ácido Pirrolidonocarboxílico/análogos & derivados , Ratos , Ratos Endogâmicos , Estimulação QuímicaRESUMO
The authors studied the anabolic effect of peptide morphogen of the hydra undecapeptide on normal and regenerating rat liver. Ornithine decarboxylase (EC 4.1.1.17) activity served as a marker. Intraperitoneal injection of the peptide into intact animals stimulated ornithine decarboxylase activity in a dose-dependent manner. In partially hepatectomized rats the peptide stimulated ornithine decarboxylase activity in the dose of 20 micrograms/kg body weight while greater doses inhibited the enzyme activity.