Microbiological and molecular identification of the anaerobic component of the oral microbiota in patients with cancer of the oropharyngeal region.

A research objective - to study tumor tissues of primary and recurrent patients with cancer of the oropharyngeal region for the frequency of occurrence of four types of anaerobic periodontogens and their associations by two methods: real-time PCR and cultural. There is speculation that bacteria can influence the pathogenesis of cancer. A comparative assessment of the content of four anaerobic periodontogens (Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Treponema denticola) in the tumor tissue and in the healthy tissue of the oral mucosa of patients with oropharyngeal cancer was carried out. It was found that the identification of odontopathogens by the real-time PCR method is much more informative than the traditional culture method, with the exception of P. intermedia, for the identification of which the traditional culture method was more effective. In 33.3% of patients, both primary and secondary, the composition of microorganisms was the same in both healthy and tumor tissue. In 20% of primary patients and in 13.3% of repeat patients, no associations of microorganisms included in the study were found in healthy tissue. Associations of 4 bacteria were recorded only in tumor tissue in both primary and repeated patients, and in repeated patients - statistically significantly more often. In 53.3% of repeat patients, associations of 4 bacteria were recorded in tumor tissue, whereas in primary patients, only in one case. P. gingivalis from tumor tissue in repeat patients was statistically significantly more often than in primary patients. T. forsythensis in primary patients was found statistically significantly more often in healthy tissues than in repeat patients, in which T. forsythensis was found statistically significantly more often from tumor tissue than in healthy tissue). T. denticola in healthy tissue was detected in both primary and repeated patients in isolated cases. T. denticola in tumor tissue was found statistically significantly more frequently in both primary and repeated patients compared to healthy tissue. P. gingivalis, T. forsythensis, and T. denticola should perhaps be considered risk indicators indicating the level of significance of their associations with oropharyngeal cancer.

[Comparative analysis of two main prognostic classifications for predicting the locally advanced prostate cancer after radical prostatectomy].

OBJECTIVE: comparison of two main clinical classifications D'Amico and NCCN for predicting the locally advanced prostate cancer. In this study we evaluated the preoperative data of 150 patients who underwent the radical prostatectomy. All patients were divided in prognostic groups according to NCCN and D'Amico classification criteria based on preoperative PSA level, digital rectal examination (DRE) and Gleason score. For comparative analysis of two main models statistical calculation was performed. Clinical application of magnetic resonance tomography is mandatory for evaluation of local extension for patients with intermediate and high risk. Comparative analysis of two main methods revealed the superiority of NCCN over D'Amico classification (p=0,0032) in predicting locally advanced prostate cancer.

[Comparative estimation of medicinal and complex therapy influence on immunological indexes in the patients with acne disease].

The aim of this work is to study the indexes of immune status and level of endogenous intoxication in patients with acne disease, which have got medicinal and complex treatment with using ozone-oxygen mixture (OOM). After complex course of treatment positive changes have happened in regulatory systems - in indexes of cellular and humoral immunity. The treatment with using OOM enabled earlier normalization of absolute and percentage content of T-lymphocytes, removed immunodeficiency of suppressor and helper population of T-lymphocytes, decreased circulating immune complexes, amount of B-lymphocytes. The latter was accompanied by normalization or increase of the level of the separate classes of immunoglobulines. Using OOM resulted in normalization of nonspecific immunity indexes, its bactericide activity. Under the influence of complex treatment of acne disease, statistically important increase of active neutrophiles amount have been observed in comparison with data before treatment and decrease of spontaneous and stimulated indexes of nitroblue-tetrozolit test.