Effect of graphene oxide on the uptake, translocation and toxicity of metal mixture to Lepidium sativum L. plants: Mitigation of metal phytotoxicity due to nanosorption.

Due to its unique structure and exceptional properties, graphene oxide (GO) is increasingly used in various fields of industry and therefore is inevitably released into the environment, where it interacts with different contaminants. However, the information relating to the ability of GO to affect the toxicity of contaminants is still limited. Therefore, the aim of our study was to synthesize GO, to examine the phytotoxicity of different concentrations of GO and its co-exposure with the metal mixture using garden cress (Lepidium sativum L.) as a test organism and to evaluate the potential of GO to affect toxicity of metals and their uptake by plants. The metal mixture (MIX) containing Ni (II), Zn (II), Cr (III) and Cu (II) was prepared in accordance with the maximum-permissible-concentrations (MPC) accepted for the inland waters in the EU. Additionally, the capacity of GO to adsorb metals was studied in specific conditions of the phytotoxicity test and assessed using adsorption isotherms. Our data indicate that in most cases the tested concentrations of MIX, GO and MIX + GO did not affect seed germination, root growth and biomass of roots and seedlings, however, they were found to alter photosynthesis processes, enhance production of carotenoids and H2O2 as well as to activate lipid peroxidation. Additionally, our study revealed that GO affects the accumulation of tested metals in roots and shoots of the MIX-exposed L. sativum. This is due to the capacity of GO to adsorb metals from the growth medium. Therefore, low concentrations of GO can be used for water decontamination.

Toxicological effects of different-sized Co-Fe (CoFe2O4) nanoparticles on Lepidium sativum L.: towards better understanding of nanophytotoxicity.

Due to their widespread therapeutic and agricultural applicability and usefulness in removing metals and metalloids from water, cobalt ferrite nanoparticles (NPs) are currently receiving increasing attention from researchers. However, their potential phytotoxicity is still poorly understood. Thus, the aim of the current study was to assess the effects of synthesized cobalt ferrite (CoFe2O4) NPs on biological (morphological, physiological, and biochemical) parameters of edible plant garden-cress (Lepidium sativum L.), depending on particle size and concentrations. In this study, physical characteristics of cobalt ferrite NPs were determined. Increased total content of Co and Fe in L. sativum tissues and their transfer from roots to above-ground parts of seedlings, which depended on the size of NP (15 < 5 < 1.65 nm), indicated that plants had been exposed to Co ferrite NPs. The relative growth of roots, biomass of roots and above-ground parts of seedlings, amounts of chlorophylls a and b, carotenoids, and malondialdehyde (MDA) were determined. The dependence of the tested garden-cress parameters on the size and concentrations of NPs was revealed. Our data showed that the content of MDA in test plants in some cases increased up to 2.5 folds in comparison to control. The increase of the content of chlorophyll b pigment and MDA in test plants is an appropriate indicator of the impact of cobalt ferrite NPs. The findings of our study into toxicological effects of Co-Fe (CoFe2O4) NPs on L. sativum are expected to deepen the knowledge of the nanophytotoxicity of ferromagnetic NPs and their potential application in biomedicine and agriculture.