The severity of kettle burns and the dangers of the dangling cord.

Kettle scalds are a major cause of paediatric burn injury. Whilst preventative measures have been advocated for years, the incidence of this type of injury is increasing. Information is lacking regarding severity and mechanism of injury. We prospectively analysed all paediatric scald injuries that warranted referral to a tertiary burn centre to quantify severity, outcome, look into how these burns occurred with a view to providing information for preventative strategies and audited the safety features of 19 top selling kettles. A total of 119 kettle burns were included in the study. Mean age at time of injury was 2 years 1 month (5 months to 12 years). Mean TBSA was 11.8% (2-30) and 62% sustained TBSA >10%. Mean length of stay was 10.4 (1-120) days and a surgical procedure was required in 62% of all kettle scalds. Grafting was undertaken in 52% of patients. Mean number of surgical procedures was 1.7 (median 1, range 1-9). Pulling the kettle cord was the main mechanism of injury (74% vs. 37%). "Cord-pullers" were also significantly younger (mean 20 months vs. 51 months, p<0.05) and underwent significantly more grafting in "cosmetically sensitive" areas (69% vs. 23%, p<0.05). 4 cases of inhalational injury occurred as a result of pulling the kettle cord. Mean kettle cord length was 66 cm (range 45-80) with only 30% employing a coil tidy feature. In view of these findings we discuss current legislative policy on kettle cord length and report on the current safety of today's kettles. Areas for future prevention are discussed.

Polymorphism at the avpr1a locus in male prairie voles correlated with genetic but not social monogamy in field populations.

Integrative studies of genetics, neurobiology and behaviour indicate that polymorphism in specific genes contributes to variation observed in some complex social behaviours. The neuropeptide arginine vasopressin plays an important role in the regulation of a variety of social behaviours, including social attachment of males to females, through its action on the vasopressin 1a receptor (V1aR). In socially monogamous prairie voles (Microtus ochrogaster), polymorphism in the length of microsatellite DNA within the regulatory region of the gene (avpr1a) encoding the V1aR predicts differences among males in neural expression of V1aRs and partner preference under laboratory conditions. However, understanding the extent to which V1aR mediates variation in prairie vole social and reproductive behaviour observed in nature requires investigating the consequences of avpr1a polymorphism and environmental influences under ecologically relevant conditions. We examined the relationship between avpr1a length polymorphism and monogamy among male prairie voles living in 0.1 ha enclosures during a time similar to their natural lifespan. We found no evidence that avpr1a genotype of males predicts variation in social monogamy measured in the field but some indices of social monogamy were affected by population density. Parentage data indicated that a male's avpr1a genotype significantly influenced the number of females with which he sired offspring and the total number of offspring sired. Total brain concentrations of V1aR mRNA were not associated with either male behaviour or avpr1a genotype. These data show that melding ecological field studies with neurogenetics can substantially augment our understanding of the effects of genes and environment on social behaviours.

Malingering on the RAVLT. Part I. Deterrence strategies.

The effect of warning regarding detection of malingering on the Rey Auditory Verbal Learning Test (RAVLT) was examined in this study. Sixty undergraduate students were randomly assigned to one of four conditions: malingerers, malingerers-with-warnings, warning-only, and control. An incentive that appeared differential, but was an actual constant reward, was offered to participants who could fake in a believable manner (for those in malingering conditions), or to those who performed to the best of their ability (non-malingering conditions). It was predicted that warning participants about the possibility that faking could be detected would modify the behaviour of malingerers, but not those instructed to perform to the best of their ability. Warning had no effect on behaviour in either condition, which was consistent with expectations for the warning-only group, but not for the malingering group. Results are discussed in terms of the ethical and legal issues associated with malingering in neuropsychological practice.

Metal content of dandelion (Taraxacum officinale) leaves in relation to soil contamination and airborne particulate matter.

The global distribution of the common dandelion (Taraxacum officinale Weber, sensu lato; Asteraceae), along with its ability to tolerate a wide range of environmental conditions, make this 'species' a particularly attractive candidate to evaluate for its value as a biological monitor of environmental metal contamination. To examine the metal content of dandelion leaves in relation to environmental metal levels, the concentrations of eight metals (Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn) were analyzed in leaf and soil samples collected at 29 sites in the mid-western United States differentially impacted by pollution. Sites were chosen primarily to cover a range of annual mean 24-h airborne particulate matter < or = 10 microm (hereafter, PM10) exposure, with PM10 levels varying from those found in isolated rural areas to levels typical of the most industrialized urban locations in the mid-western United States. A positive. significant correlation was detected between soil concentrations of each metal and measures of PM10 at a site, signifying that airborne particulate matter is a good indicator of soil metal contamination. Leaf concentrations of four of the eight metals (Cr, Mn, Pb and Zn) examined were found to increase significantly as the soil levels of these metals increased, but the percentage of the total variation explained by the relationship in these cases was generally low. This latter finding, along with the lack of a significant relationship between leaf and soil concentrations for the four other metals, indicate that the factors affecting metal absorption from the soil by dandelions are complex and that, aside from soil metal concentrations, other soil, plant and/or other environmental factors affect metal uptake. There was also no evidence that leaf metal concentrations were positively correlated with PM10. In addition, the concentrations of some metals (Cu, Fe, Mn, Pb and Zn) were significantly higher in leaves collected in the fall compared to those collected at the same sites in the spring. These findings suggest that dandelions may not be a particularly effective tool for quantifying levels of environmental metal contamination, at least on the scale of pollution typifying industrialized urban areas of the mid-western United States.

Genetic diversity of Typha latifolia (Typhaceae) and the impact of pollutants examined with tandem-repetitive DNA probes.

Genetic diversity at variable-number-tandem-repeat (VNTR) loci was examined in the common cattail, Typha latifolia (Typhaceae), using three synthetic DNA probes composed of tandemly repeated "core" sequences (GACA, GATA, and GCAC). The principal objectives of this investigation were to determine whether: (1) the previously reported almost complete lack of polymorphism at allozyme loci in this species was indicative of a reduced amount of genetic diversity at VNTR loci as well; (2) VNTR markers were informative about possible clonal propagation; and (3) significant differences in genetic structure of sampling sites were associated with differences in environmental levels of pollutants at those sites. Previously, widespread sampling across the eastern United States, surveying across ten allozyme loci, has detected only two genotypes, involving a difference at a single locus, among 104 populations. In this study, the amount of genetic diversity detected at VNTR loci: (1) among ramets (N = 40; 40 genotypes detected) collected at ∼8-km intervals along a 320-km transect; (2) among ramets (N = 220; 117 genotypes detected) from five study sites separated by 50-3000 m; and (3) even among ramets within each study site [N = 44 per site; from 13 to 34 genotypes detected per site (270 m(2))] exceeds that previously found in those more geographically widespread allozyme surveys. Among the 260 ramets analyzed here, the mean number of bands scored per individual was 48.61 (SD = 2.80). Mean genetic similarity among ramets collected along the 320-km transect was 0.91, which was within the range of mean genetic similarity within the five study sites (range: 0.89-0.95). Among the five study sites, 61% of the samples analyzed appeared to be clonal ramets, with up to 12 clones detected for 44 ramets sampled within a site. Clones grew intermingled and ranged up to 39 m in extent. Permutation tests of genetic similarity revealed significant genetic differentiation between each of the five study sites. Consistent with the previous allozyme studies, T. latifolia was characterized by extremely low genetic variation relative to levels of polymorphism detected at VNTR loci in other plant species. Estimated heterozygosity among ramets along the 320-km transect ranged from 0.11 to 0.13, while that within the five study sites ranged from 0.05 to 0.12. Estimates of F(st) (0.32-0.41) also indicated considerable genetic subdivision among these stands. Significantly higher genetic diversity was detected at the two study sites that chemistry and toxicity data indicate to be the most severely impacted by pollutants. Although this correlation does not establish cause and effect, the results of this study indicate that the analysis of genetic diversity at VNTR loci may be a useful tool for monitoring anthropogenic-induced changes in the genetic structure of natural populations of plants.

Paternity assessment in wild groups of toque macaques Macaca sinica at Polonnaruwa, Sri Lanka using molecular markers.

Genetic variation at four microsatellite loci in conjunction with that at a highly variable allozyme locus was used to analyse paternity over a 12-year period in 13 social groups of toque macaques Macaca sinica inhabiting a natural forest in Polonnaruwa, Sri Lanka. Paternity exclusion analysis revealed that the set of offspring produced by a female usually consists of half-siblings because few males father more than one offspring with a particular female. No evidence of offspring produced by matings between first degree relatives was found. The social unit in toque macaques was not identical to the reproductive unit and the possibility of paternity by males outside the social group should be considered when estimating male reproductive output. Although it was common for multiple males to father offspring in a social group each year, reproduction within a group during a breeding season tended to be limited to a few males. The mean number of males reproducing per group per year was independent of the number of males in a group. The paternity data suggests that many males may father relatively few offspring during their entire lives and that the effective population size for toque macaques may be much smaller than indicated by demographic data.

Rodent models of alcoholism: a review.

Four criteria should be fulfilled when establishing an animal model of alcoholism. (1) The animal should voluntarily and selectively consume a solution of the drug to yield pharmacologically significant blood alcohol concentrations. When ethanol is consumed for several weeks under these conditions, (2) the animals should exhibit tolerance to the acute effects of the drug. Furthermore, (3) abrupt termination of drug administration should result in physical signs of drug withdrawal (e.g. audiogenic seizures). (4) Changes in endocrine and liver function would also be expected to occur following the chronic administration of alcohol, such effects being particularly pronounced if the diet of the animal is nutritionally deficient. A number of self-administration, operant conditioning and forced alcohol administration models are critically assessed. A detailed description of the models in which rodents are forced to consume increasing quantities of alcohol as part of a nutritionally enriched milk diet suggests that this method fulfils most of the above criteria.

Maternal syndrome associated with hydrops fetalis: case report.

A case of maternal fluid retention syndrome associated with fetal hydrops due to rhesus isoimmunisation is reported. The aetiology and clinical features are discussed. Prophylactic anti-Rh immunoglobin should reduce the incidence of such cases in the future.