Toward site-specific and self-sufficient robotic fabrication on architectural scales.

Contemporary construction techniques are slow, labor-intensive, dangerous, expensive, and constrained to primarily rectilinear forms, often resulting in homogenous structures built using materials sourced from centralized factories. To begin to address these issues, we present the Digital Construction Platform (DCP), an automated construction system capable of customized on-site fabrication of architectural-scale structures using real-time environmental data for process control. The system consists of a compound arm system composed of hydraulic and electric robotic arms carried on a tracked mobile platform. An additive manufacturing technique for constructing insulated formwork with gradient properties from dynamic mixing was developed and implemented with the DCP. As a case study, a 14.6-m-diameter, 3.7-m-tall open dome formwork structure was successfully additively manufactured on site with a fabrication time under 13.5 hours. The DCP system was characterized and evaluated in comparison with traditional construction techniques and existing large-scale digital construction research projects. Benefits in safety, quality, customization, speed, cost, and functionality were identified and reported upon. Early exploratory steps toward self-sufficiency-including photovoltaic charging and the sourcing and use of local materials-are discussed along with proposed future applications for autonomous construction.

3D Printed Multimaterial Microfluidic Valve.

We present a novel 3D printed multimaterial microfluidic proportional valve. The microfluidic valve is a fundamental primitive that enables the development of programmable, automated devices for controlling fluids in a precise manner. We discuss valve characterization results, as well as exploratory design variations in channel width, membrane thickness, and membrane stiffness. Compared to previous single material 3D printed valves that are stiff, these printed valves constrain fluidic deformation spatially, through combinations of stiff and flexible materials, to enable intricate geometries in an actuated, functionally graded device. Research presented marks a shift towards 3D printing multi-property programmable fluidic devices in a single step, in which integrated multimaterial valves can be used to control complex fluidic reactions for a variety of applications, including DNA assembly and analysis, continuous sampling and sensing, and soft robotics.

DNA Assembly in 3D Printed Fluidics.

The process of connecting genetic parts-DNA assembly-is a foundational technology for synthetic biology. Microfluidics present an attractive solution for minimizing use of costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro- and millifluidic devices are inexpensive, easy to make and quick to produce. We demonstrate Golden Gate DNA assembly in 3D-printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 microns, and per unit part costs ranging from $0.61 to $5.71. A 3D-printed syringe pump with an accompanying programmable software interface was designed and fabricated to operate the devices. Quick turnaround and inexpensive materials allowed for rapid exploration of device parameters, demonstrating a manufacturing paradigm for designing and fabricating hardware for synthetic biology.

Multi-scale thermal stability of a hard thermoplastic protein-based material.

Although thermoplastic materials are mostly derived from petro-chemicals, it would be highly desirable, from a sustainability perspective, to produce them instead from renewable biopolymers. Unfortunately, biopolymers exhibiting thermoplastic behaviour and which preserve their mechanical properties post processing are essentially non-existent. The robust sucker ring teeth (SRT) from squid and cuttlefish are one notable exception of thermoplastic biopolymers. Here we describe thermoplastic processing of squid SRT via hot extrusion of fibres, demonstrating the potential suitability of these materials for large-scale thermal forming. Using high-resolution in situ X-ray diffraction and vibrational spectroscopy, we elucidate the molecular and nanoscale features responsible for this behaviour and show that SRT consist of semi-crystalline polymers, whereby heat-resistant, nanocrystalline ß-sheets embedded within an amorphous matrix are organized into a hexagonally packed nanofibrillar lattice. This study provides key insights for the molecular design of biomimetic protein- and peptide-based thermoplastic structural biopolymers with potential biomedical and 3D printing applications.