Cataract surgery in camp patients: a study on visual outcomes.

AIM: To assess the complications and visual outcomes associated with cataract surgery in camp patients operated at a tertiary centre. MATERIALS AND METHODS: In a retrospective study, 206 outreach camp patients had undergone cataract surgeries with posterior chamber intraocular lens implantation under peribulbar anesthesia over a period of 6 months. Post-operative complications on Day 1 were graded as per Oxford Cataract Treatment and Evaluation Team (OCTET) definitions. One month postoperative complications, best corrected visual acuity and refractive errors were assessed. RESULTS: 206 eyes underwent cataract extraction with PCIOL implantation. Small incision cataract surgery (SICS) was the commonest method (78.6%) used. The most common first post-operative day complication was mild iridocyclitis (26.2%). The complications were based on OCTET definitions, and showed that 33 % had Grade I and 3.4 % had Grade II complications. The major post-operative complication after 4 weeks of surgery was posterior capsular opacity. 89.8 % of the eyes had a 4 week-post-operative best corrected visual acuity of e± 6/24. The commonest refractive error was myopia with against the rule astigmatism, seen in 86 out of 150 cases. CONCLUSION: High quality cataract surgery with a low rate of intra-operative complications and good visual outcome can be attained in camp patients operated in the base hospitals, thus justifying more similar screening camps to clear the vast cataract backlog.

Differential expression of thermophilic phosphatases in the wild type and auxotrophic mutant strains of Thermoactinomyces vulgaris.

In the wild type strain (stock no. 1227) of Thermoactinomyces vulgaris, as reported earlier [Sinha and Singh (1980) Biochem. J. 190, 457-460], all phosphatase isoenzymes (three alkaline - AlpI, AlpII and AlpIII, and one acidic - Acp) are present. However, the auxotrophic mutants, the strains 1286 (thi(-)), 1279 (nic(-), ura(-)) and 1278 (thi(-), ura(-)) exhibited two alkaline phosphatase isoenzymes (AlpII and AlpIII), but AlpI was lacking. In the strain 1261 (nic(-), thi(-)), only AlpIII was expressed, and AlpI and AlpII isoenzymes were missing. The results suggest that the strains, which require either thiamine (1286 and 1278) or nicotinamide (1279) for their growth, were AlpI(-) mutants; and the strain (1261), which requires both thiamine and nicotinamide for its growth, was AlpI(-)/AlpII(-) double mutant. There was no direct correlation between uracil auxotrophy and the expression of phosphatases. The uniform expression of AlpIII and Acp in all the strains, irrespective of their nutrient requirements, suggest that these constitutive phosphatases are species-specific. The specific activities of the thermophilic acid and alkaline phosphatases were maximum in the wild type strain (1227) of T. vulgaris. The next in phosphatase activity was the strain 1279 (an AlpI(-) mutant), followed by their decrease, in order, in the strains 1286 and 1278 (which were also AlpI(-) mutants); while least activity of these enzymes was observed in the obligate thermophile strain 1261 (AlpI(-)/AlpII(-) double mutant).