In vivo and in vitro study of a novel nanohydroxyapatite sonocoated scaffolds for enhanced bone regeneration.

There still remains a need for new methods of healing large bone defects, i.e., gaps in bone tissue that are too big to naturally heal. Bone regrowth scaffolds can fill the bone gap and enhance the bone regeneration by providing cells with a support to for new tissue formation. Coating of the scaffolds surface with nanocrystalline hydroxyapatite may enhance the osteoinductivity or osteoconductivity of such scaffolds. Here we present the sonocoating method to coat scaffolds with bioactive hydroxyapatite nanoparticles. We show a method, where the material to be coated is immersed in a colloidal suspension of nanoparticles with mean sizes of 10 nm and 43 nm in water, and high-power ultrasound waves are applied to the suspension for 15 min at 30 °C. High power ultrasounds lead to growth of cavitation bubbles in liquid, which implode at a critical size. The implosion energy propels the nanoparticles towards the material surface, causing their attachment to the scaffold. Using this technique, we produced a uniform layer of nanohydroxyapatite particles of thickness in the range 200 to 300 nm on two types of scaffolds: a porous ß-TCP ceramic scaffold and a 3D-printed scaffold made of PCL fibers. In vivo tests in rabbits confirmed that the novel coating strongly stimulated new bone tissue formation, with new bone tissue occupying 33% for the nHAP-coated PCL scaffold and 68% for the nHAP-coated ß-TCP after a 3-month test. The sonocoating method leads to formation of a bioactive layer on the scaffolds at temperature close to room temperature, very short time and in water. It is a green technological process, promising for bone tissue regeneration applications.

Unique dynamic crossover in supercooled x,3-dihydroxypropyl acrylate (x = 1, 2) isomers mixture.

The previtreous dynamics in the glass-forming monomer, glycerol monoacrylate (GMA), was tested using the broadband dielectric spectroscopy (BDS). The measurements revealed a clear dynamic crossover at the temperature [Formula: see text] K and the time scale [Formula: see text] ns for the primary (structural) relaxation time and no hallmarks for the crossover for the DC electric conductivity [Formula: see text]. This result was revealed via the derivative-based and distortions-sensitive analysis [Formula: see text] vs. [Formula: see text] , where [Formula: see text] stands for the apparent activation energy. Subsequent tests of the fractional Debye-Stokes-Einsten relation [Formula: see text] showed that the crossover is associated with [Formula: see text] [Formula: see text] (for [Formula: see text]. The crossover coexists with the emergence of the secondary beta relaxation, which smoothly develops deeply into the solid amorphous phase below the glass temperature [Formula: see text].