Effects of Sugar Beet Silage, High-Moisture Corn, and Corn Silage Feed Supplementation on the Performance of Dairy Cows with Restricted Daily Access to Pasture.

A study was undertaken to assess the effect of supplementation with sugar beet silage, corn silage, or high-moisture corn on dairy performance, rumen, and plasma metabolites in dairy cows under conditions of restricted grazing in spring. Eighteen multiparous Holstein Friesian cows, stratified for milk yield (39.4 kg/day ± 3.00), days of lactation (67.0 days ± 22.5), live weight (584 kg ± 38.0), and number of calves (5.0 ± 1.5), were allocated in a replicated 3 × 3 Latin square design. Treatments were as follows: SBS (10 kg DM of permanent pasture, 7 kg DM of sugar beet silage, 4 kg DM of concentrate, 0.3 kg DM of pasture silage, 0.21 kg of mineral supplement); corn silage (10 kg DM of permanent pasture, 7 kg DM of corn silage, 4 kg DM of concentrate, 0.3 kg DM of pasture silage, 0.21 kg of mineral supplement), and HMC (10 kg DM of permanent pasture, 5 kg DM of high-moisture corn, 4.5 kg DM of concentrate, 1.2 kg DM of pasture silage, 0.21 kg of mineral supplement). Pasture was offered rotationally from 9 a.m. to 4 p.m. Between afternoon and morning milking, the cows were housed receiving a partial mixed ration and water ad libitum. The effect of treatments on milk production, milk composition, body weight, rumen function, and blood parameters were analyzed using a linear−mixed model. Pasture dry matter intake (DMI) was lower in SBS than CS (p < 0.05) and similar to HMC, but total DMI was higher in HMC than SBS (p < 0.05) and similar to CS. Milk production for treatments (32.6, 31.7, and 33.4 kg/cow/day for SBS, CS, and HMC, respectively), live weight, and fat concentration were not modified by treatments, but milk protein concentration was lower for SBS compared with HMC (p < 0.05) and similar to CS. B-hydroxybutyrate, cholesterol, and albumin were not different among treatments (p > 0.05), while urea was higher in SBS, medium in CS silage, and lower in HMC (p < 0.001). Ruminal pH and the total VFA concentrations were not modified by treatments (p > 0.05), which averaged 6.45 and 102.03 mmol/L, respectively. However, an interaction was observed for total VFA concentration between treatment and sampling time (p < 0.05), showing that HMC produced more VFA at 10:00 p.m. compared with the other treatments. To conclude, the supplementation with sugar beet silage allowed a milk response and composition similar to corn silage and HMC, but with a lower concentration of milk protein than HMC. In addition, sugar beet silage can be used as an alternative supplement for high-producing dairy cows with restricted access to grazing during spring.

Effect of dietary inclusion of winter brassica crops on milk production, feeding behavior, rumen fermentation, and plasma fatty acid profile in dairy cows.

This study determined feeding behavior, dry matter (DM) intake (DMI), rumen fermentation, and milk production responses of lactating dairy cows fed with kale (Brassica oleracea) or swede (Brassica napus ssp. napobrassica). Twelve multiparous lactating dairy cows (560 ± 22 kg of body weight, 30 ± 4 kg of milk/d, and 60 ± 11 d in milk at the beginning of the experiment; mean ± standard deviation) were randomly allocated to 3 dietary treatments in a replicated 3 × 3 Latin square design. The control diet comprised 10 kg of grass silage DM/d, 4 kg of ryegrass herbage DM/d, and 8.8 kg of concentrate DM/d. Then, 25% of herbage, silage, and concentrate (DM basis) was replaced with either kale or swede. Cows offered kale had decreased total DMI compared with cows fed the control and swede diets, whereas inclusion of swede increased eating time. Milk production, composition, and energy-corrected milk:DMI ratio were not affected. Cows fed with kale had a greater rumen acetate:propionate ratio, whereas swede inclusion increased the relative percentage of butyrate. Estimated microbial N was not affected by dietary treatments, but N excretion was reduced with inclusion of kale, improving N utilization. Cows fed kale tended to have increased nonesterified fatty acids and showed presence of Heinz-Ehrlich bodies, whereas hepatic enzymes such as aspartate aminotransferase, γ-glutamyl transferase, and glutamate dehydrogenase were not affected by dietary treatments. In plasma, compared with the control, swede and kale reduced total saturated fatty acids and increased total polyunsaturated fatty acids and total n-3 fatty acids. Overall, feeding cows with winter brassicas had no negative effect on production responses. However, mechanisms to maintain milk production were different. Inclusion of swede increased the time spent eating and maintained DMI with a greater relative rumen percentage of butyrate and propionate, whereas kale reduced DMI but increased triacylglycerides mobilization, which can negatively affect reproductive performance. Thus, the inclusion of swede may be more suitable for feeding early-lactating dairy cows during winter.

Chemical Composition, Fatty Acid Profile and Sensory Characteristics of Chanco-Style Cheese from Early Lactation Dairy Cows Fed Winter Brassica Crops.

Brassica crops such as kale and swede can be supplied to cow diets during winter, however little is known about the effects of feeding those forage brassicas to lactating cows on cheese nutritional characteristics of milk and cheese. This study evaluated the effect of including kale or swede in pasture-fed lactating dairy cow diets on chemical composition, fatty acid (FA) profile, and sensory characteristics of Chanco-style cheese. Twelve early-lactation cows were used in a replicated (n = 4) 3 × 3 square Latin square design. The control diet consisted of (DM basis) 10.0 kg of grass silage, 4.0 kg of fresh grass pasture, 1.5 kg soybean meal, 1.0 kg of canola meal, and 4.0 kg of cereal-based concentrate. The other treatments replaced 25% of the diet with swede or kale. Milk yield, milkfat, and milk protein were similar between treatments as were cheese moisture, fat, and protein. Swede and kale increased total saturated cheese FA while thrombogenic index was greater in swede, but color homogeneity and salty flavor were greater while ripe cheese aroma less than for kale. Kale or swede can be used in the diet of pasture-fed lactating dairy cows without negative effects on milk production, milk composition, or cheese composition. However, kale and swede increased total cheese saturated FA.

In Vitro Fermentation Patterns and Methane Output of Perennial Ryegrass Differing in Water-Soluble Carbohydrate and Nitrogen Concentrations.

The objective of this study was to determine the effect of perennial ryegrass (PRG) forages differing in their concentration of water-soluble carbohydrates (WSC) and crude protein (CP), and collected in spring and autumn, on in vitro rumen fermentation variables, nitrogen (N) metabolism indicators and methane (CH4) output, using a batch culture system. Two contrasting PRG pastures, sampled both in autumn and spring, were used: high (HS) and low (LS) sugar pastures with WSC concentrations of 322 and 343 g/kg for HS (autumn and spring), and 224 and 293 g/kg for LS in autumn and spring, respectively. Duplicates were incubated for 24 h with rumen inocula in three different days (blocks). Headspace gas pressure was measured at 2, 3, 4, 5, 6, 8, 10, 12, 18, and 24 h, and CH4 concentration was determined. The supernatants were analysed for individual volatile fatty acids (VFA) concentrations, and NH3-N. The solid residue was analysed for total N and neutral detergent insoluble N. Another set of duplicates was incubated for 4 h for VFA and NH3-N determination. The HS produced more gas (218 vs. 204 mL/g OM), tended to increase total VFA production (52.0 mM vs. 49.5 mM at 24 h), reduced the acetate:propionate ratio (2.52 vs. 3.20 at 4 h and 2.85 vs. 3.19 at 24 h) and CH4 production relative to total gas production (15.6 vs. 16.8 mL/100 mL) and, improved N use efficiency (22.1 vs. 20.9). The contrasting chemical composition modified in vitro rumen fermentation tending to increase total VFA production, reduce the acetate:propionate ratio and CH4 concentration, and improve N use efficiency through lower rumen NH3-N.

Evaluation of sources of variation on in vitro fermentation kinetics of feedstuffs in a gas production system.

The aim of this study was to evaluate the effect of different sources of variation in gas production technique on the in vitro gas production kinetics of feedstuffs. Triplicates of commercial concentrate, grass silage, grass hay and grass pasture were incubated in three experiments: experiment 1 assessed two agitation methods; experiment 2 evaluated different rumen inocula (pooled or different donor cows for each incubation run); and experiment 3 used Goering-Van Soest or Mould buffers for media preparation. Gas production data were fitted into the Michaelis-Menten model and then subjected to analysis of variance. Gas production (GP) at 48 h and asymptote gas production (A) were lower when bottles were continuously under horizontal movement. Time to produce half and 75% of A, and A were affected by rumen inocula, while buffer type affected time to produce half and 25% of A and GP. No interactions between substrates and sources of variation were observed, suggesting that the effects of substrates on GP parameters were not modified. It is concluded that comparison of numerical data from in vitro experiments that follow different protocols must be done carefully. However, the ranking of different substrates is more robust and less affected by the sources of variation.