RESUMO
Rapid microtiter plate-based colorimetric assays have been developed that allow the screening of large numbers of samples for the presence of inhibitors of alpha-glucosidase, alpha-amylase, and beta-galactosidase. The assays are particularly useful for screening large numbers of microbial culture filtrates.
Assuntos
Inibidores de Glicosídeo Hidrolases , alfa-Amilases/antagonistas & inibidores , beta-Galactosidase/antagonistas & inibidores , Avaliação Pré-Clínica de Medicamentos , Fatores de Tempo , TitulometriaRESUMO
Culture filtrates and organic solvent extracts of over 500 freshwater and marine eukaryotic microalgae and cyanobacteria were screened for the presence of glycosidase inhibitors. Rapid colorimetric assays were used to detect inhibitors of alpha-glucosidase, alpha-amylase and beta-galactosidase. Inhibitors were found from 38 species. The results suggest that microalgae and cyanobacteria have potential as a source of glycosidase inhibitors which may have clinical applications.
