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1.
PLoS Negl Trop Dis ; 18(7): e0012329, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38990803

RESUMO

[This corrects the article DOI: 10.1371/journal.pntd.0001331.].

2.
PLoS Negl Trop Dis ; 8(5): e2872, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24854034

RESUMO

Scabies is an infectious skin disease caused by the mite Sarcoptes scabiei and has been classified as one of the six most prevalent epidermal parasitic skin diseases infecting populations living in poverty by the World Health Organisation. The role of the complement system, a pivotal component of human innate immunity, as an important defence against invading pathogens has been well documented and many parasites have an arsenal of anti-complement defences. We previously reported on a family of scabies mite proteolytically inactive serine protease paralogues (SMIPP-Ss) thought to be implicated in host defence evasion. We have since shown that two family members, SMIPP-S D1 and I1 have the ability to bind the human complement components C1q, mannose binding lectin (MBL) and properdin and are capable of inhibiting all three human complement pathways. This investigation focused on inhibition of the lectin pathway of complement activation as it is likely to be the primary pathway affecting scabies mites. Activation of the lectin pathway relies on the activation of MBL, and as SMIPP-S D1 and I1 have previously been shown to bind MBL, the nature of this interaction was examined using binding and mutagenesis studies. SMIPP-S D1 bound MBL in complex with MBL-associated serine proteases (MASPs) and released the MASP-2 enzyme from the complex. SMIPP-S I1 was also able to bind MBL in complex with MASPs, but MASP-1 and MASP-2 remained in the complex. Despite these differences in mechanism, both molecules inhibited activation of complement components downstream of MBL. Mutagenesis studies revealed that both SMIPP-Ss used an alternative site of the molecule from the residual active site region to inhibit the lectin pathway. We propose that SMIPP-Ss are potent lectin pathway inhibitors and that this mechanism represents an important tool in the immune evasion repertoire of the parasitic mite and a potential target for therapeutics.


Assuntos
Lectina de Ligação a Manose da Via do Complemento/efeitos dos fármacos , Sarcoptes scabiei/enzimologia , Serina Proteases/metabolismo , Serina Proteases/farmacologia , Sequência de Aminoácidos , Animais , Complemento C1q/antagonistas & inibidores , Complemento C1q/metabolismo , Interações Hospedeiro-Patógeno , Humanos , Lectina de Ligação a Manose/antagonistas & inibidores , Lectina de Ligação a Manose/metabolismo , Serina Proteases Associadas a Proteína de Ligação a Manose/metabolismo , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Mutação , Escabiose/metabolismo , Alinhamento de Sequência , Serina Proteases/química , Serina Proteases/genética
3.
PLoS Negl Trop Dis ; 5(9): e1331, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21980545

RESUMO

BACKGROUND: Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families located in the mite gut as crucial factors for host-parasite interactions. Among these multiple proteins inhibit human complement, presumably to avoid complement-mediated damage of gut epithelial cells. Peritrophins are major components of the peritrophic matrix often found in the gut of arthropods. We hypothesized that a peritrophin, if abundant in the scabies mite gut, could be an activator of complement. METHODOLOGY/PRINCIPAL FINDINGS: A novel full length scabies mite peritrophin (SsPTP1) was identified in a cDNA library from scabies mites. The amino acid sequence revealed four putative chitin binding domains (CBD). Recombinant expression of one CBD of the highly repetitive SsPTP1 sequence as TSP-hexaHis-fusion protein resulted in soluble protein, which demonstrated chitin binding activity in affinity chromatography assays. Antibodies against a recombinant SsPTP1 fragment were used to immunohistochemically localize native SsPTP1 in the mite gut and in fecal pellets within the upper epidermis, co-localizing with serum components such as host IgG and complement. Enzymatic deglycosylation confirmed strong N- and O-glycosylation of the native peritrophin. Serum incubation followed by immunoblotting with a monoclonal antibody against mannan binding lectin (MBL), the recognition molecule of the lectin pathway of human complement activation, indicated that MBL may specifically bind to glycosylated SsPTP1. CONCLUSIONS/SIGNIFICANCE: This study adds a new aspect to the accumulating evidence that complement plays a major role in scabies mite biology. It identifies a novel peritrophin localized in the mite gut as a potential target of the lectin pathway of the complement cascade. These initial findings indicate a novel role of scabies mite peritrophins in triggering a host innate immune response within the mite gut.


Assuntos
Interações Hospedeiro-Parasita , Imunidade Inata , Proteínas de Insetos/imunologia , Sarcoptes scabiei/imunologia , Escabiose/imunologia , Animais , Quitina/metabolismo , Cromatografia de Afinidade , Proteínas do Sistema Complemento/imunologia , DNA Complementar/química , DNA Complementar/genética , Trato Gastrointestinal/química , Trato Gastrointestinal/imunologia , Humanos , Proteínas de Insetos/genética , Dados de Sequência Molecular , Ligação Proteica , Estrutura Terciária de Proteína , Sarcoptes scabiei/genética , Análise de Sequência de DNA
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