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1.
Z Exp Psychol ; 46(2): 129-34, 1999.
Artigo em Alemão | MEDLINE | ID: mdl-10321130

RESUMO

Script-animation, a new method for prototypically describing and replicating natural movements of the human body, is introduced in this paper. The principal elements are the technique for producing scripts of behavior through biomechanical taxonomy, and the digital interface, which feeds the positional codes to the respective body structures of a computer doll. Illustrating the new method, all gestural movements from 59 political leaders as they appeared in TV-broadcasts news were reanimated on a simple doll. 84 subjects viewed the original sequences and 36 subjects viewed the reanimations. Simultaneously, the psychophysiological responses of the subjects were recorded. A comparison of physiological parameters shows high similarity in vegetative judgement.


Assuntos
Simulação por Computador , Movimento/fisiologia , Psicofisiologia , Fenômenos Biomecânicos , Feminino , Resposta Galvânica da Pele/fisiologia , Frequência Cardíaca/fisiologia , Humanos , Masculino , Fenômenos Fisiológicos Respiratórios , Interface Usuário-Computador
2.
Appl Biochem Biotechnol ; 75(1): 129-37, 1998 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10214701

RESUMO

A panel of monoclonal antibodies was generated against the urea-based hapten N-(2-N-chloroacetylaminobenzyl)-N'-4-chlorophenylurea as a tool for building up sensitive immune assays to detect urea derivatives and to screen them for catalytic antibodies (Abs). Eleven hybridomas were obtained that produced Abs reactive to the hapten. All Abs were of IgG class. Cross reactivities of the Abs to different haptens were examined, especially to a possible transition-state analog. Only four of the hybridomas (R2-DA10/F7, R2-GE7/H2, R2-HC2/A5, R2-HD6/F7) produced Abs crossreactive with the transition-state analog. From the 11 hybridomas, hybridoma B76-BF5 was chosen for further characterization. Compared to the other Abs, B76-BF5 showed the strongest binding and had a rather restricted specificity. These Abs could be used to build up a sensitive enzyme immunoassay for the detection of the hapten. All Abs were screened for crossreactivity with the pesticides monuron and diuron. No reactivity could be detected. In addition, the nucleotide sequences of the variable light and heavy chain genes of the similarly reactive Abs B76-BF5, B76-BB3, R2-DA10/F7, and R2-GA6/G3 were determined to clarify whether structure and binding specificity of these Abs showed any correlation.


Assuntos
Acetamidas/imunologia , Anticorpos Monoclonais/isolamento & purificação , Praguicidas/imunologia , Compostos de Fenilureia/imunologia , Ureia/análogos & derivados , Ureia/imunologia , Sequência de Aminoácidos , Animais , Reações Cruzadas , Técnicas Imunoenzimáticas , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular
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