Zinc status and cognitive function of pregnant women in Southern Ethiopia.

The relation between zinc status and cognitive function was examined in a cross-sectional study in the Sidama area of Southern Ethiopia. Pregnant women >24 weeks of gestation from three adjacent rural villages volunteered to participate. Mean (s.d.) plasma zinc of 99 women was 6.97 (1.07) mumol/l (below the cutoff of 7.6 mumol/l indicative of zinc deficiency at this stage of gestation). The Raven's Coloured Progressive Matrices (CPM) test was administered individually. Scores for the Raven's scale A, which is the simplest scale, ranged from 4 to 10 of a possible 12. Women with plasma zinc <7.6 mumol/l had significantly lower Raven's CPM scale A scores than women with plasma zinc concentrations >7.6 mumol/l. Plasma zinc and maternal age and education predicted 17% of the variation in Raven's CPM scale A scores. We conclude that zinc deficiency is a major factor affecting cognition in these pregnant women.

Red blood cell folate and serum vitamin B12 status in children with sickle cell disease.

PURPOSE: To determine red blood cell (RBC) folate and serum vitamin B12 levels in children with sickle cell disease, SS-type, and to evaluate the associations of these nutrient levels with growth and hematologic parameters. PATIENTS AND METHODS: Subjects enrolled in this prospective, cross-sectional study were recruited from one tertiary care setting. Complete blood counts, measurement of red blood cell (RBC) folate and serum vitamin B12, anthropometric measures (height, weight, skinfold measurements), pubertal status, and 24-hour dietary recalls were obtained from 70 patients ages 1 to 19 years. RESULTS: Low RBC folate levels were found in 15% of the children. Fifty-seven percent of the sample had inadequate dietary folate intake. Three percent of the children had low serum vitamin B12 levels. All children and adolescents sampled had adequate dietary intake of vitamin B12. Both RBC folate (P = 0.01) and serum vitamin B12 levels (P < 0.01) decreased with increasing age. CONCLUSIONS: More than half of the subjects had inadequate intake of folate from food, and despite daily folate supplementation, 15% had low RBC folate levels. Low serum vitamin B12 levels were rare, and dietary vitamin B12 intake was adequate. Additional research is needed to explore the effects of improved folate status, the need for folate supplementation, and the relationship of folate, vitamin B12, and homocysteine levels and the risk for vascular damage and stroke in children with sickle cell disease.

Growth patterns and nutritional factors associated with increased head circumference at 18 months in normally developing, low-birth-weight infants.

OBJECTIVE: To identify patterns of growth, nutrition practices, and feeding behaviors associated with normal Denver II developmental screening results at 18 months of age. DESIGN: A retrospective review of hospital medical charts was conducted to collect discharge diagnosis and dietary data. Developmental clinic charts were reviewed to collect growth and diet data, Denver II results, and medical data from 4-, 9-, and 18-month visits. SUBJECTS/SETTING: Twenty-eight children were identified from a review of developmental clinic records in a midwestern children's hospital. Criteria were single or twin birth, birth weight of 2,000 g or less, follow up at the hospital developmental clinic, and completion of 3 screening visits. STATISTICAL ANALYSES PERFORMED: National standards were used to calculate z scores. Patterns of growth were analyzed using analysis of variance. Stepwise forward linear regression analysis was done to construct a model predicting head circumference at 18 months of age. RESULTS: Significant catch-up growth (P = .0006) occurred as length z score increased from -0.87 at 4 months of age to -0.45 at 18 months. A regression model predicting head circumference at age 18 months was developed that included nutrition and growth variables such as head circumference at 9 months, type of parenteral amino acids administered in the hospital, z score of weight at 4 months' adjusted age, and time of first enteral feeding. APPLICATIONS: Interventions designed to support breast-feeding in the hospital and at home are indicated as is increased emphasis on monitoring growth. Delays in growth need to be treated aggressively; for example, nutrition interventions should begin by the age of 4 months if weight gain is not appropriate.

The use of gamma radiation for the elimination of Salmonella from frozen meat.

The use of a gamma radiation process for the elimination of Salmonella from frozen meat is considered with particular reference to the treatment of boned-out horsemeat and kangaroo meat imported into the UK and intended for use as pet meat.Examination of dose/survival curves produced for several serotypes of Salmonella in frozen meat shows that a radiation dose of 0.6 Mrad. will reduce a population by at least a factor of 10(5). The influence on the radiation resistance of salmonellas of such factors as preirradiation growth in the meat and temperature during irradiation have been examined and considered. It is also demonstrated with both preinoculated and naturally contaminated meat that postirradiation storage in the frozen state does not lead to the revival of irradiated salmonellas.The properties of Salmonella survivors deliberately produced in meat using conditions of irradiation designed to simulate a commercial process are studied after six recycling treatments through the process. There were no important changes in characteristics normally used for identification of Salmonella but radiation resistance was lowered. Survivors grown in situ in meat after irradiation showed an abnormally long lag phase, and removal of competitive microflora in meat by the radiation treatment can influence the growth of salmonellas.