Simultaneous determination of lamivudine and zidovudine concentrations in human seminal plasma using high-performance liquid chromatography and tandem mass spectrometry.

A HPLC-MS-MS method was developed and validated to measure lamivudine and zidovudine simultaneously in small volumes of human seminal plasma. Sample preparation was simple and rapid, requiring 25 microl of sample, the use of isotopically labeled lamivudine and zidovudine as internal standards and ultrafiltration through a molecular mass cut-off membrane. Lamivudine and its internal standard were separated from zidovudine and its internal standard with isocratic HPLC. Detection was carried out using tandem mass spectrometry. This validated method was used to analyze seminal samples obtained from six HIV-positive patients prescribed lamivudine and zidovudine.

Simultaneous determination of zidovudine and lamivudine in human serum using HPLC with tandem mass spectrometry.

A method employing high performance liquid chromatography (HPLC) with tandem mass spectrometry (MS) has been developed and validated for the simultaneous determination of clinically relevant levels of zidovudine (AZT) and lamivudine (3TC) in human serum. The method incorporates a fully automated ultrafiltration sample preparation step that replaces the solid-phase extraction step typically used for HPLC with UV detection. The calibration range of the dual-analyte LC-MS/MS method is 2.5-2,500 and 2.5-5,000 ng ml-1 for AZT and 3TC, respectively, using 0.25 ml of human serum. The lower limit of quantification was 2.5 ng ml-1 for each analyte, with a chromatographic run time of approximately 6 min. Overall accuracy, expressed as bias, and inter- and intra-assay precision are < +/- 7 and < 10% for AZT, and < +/- 5 and < 12.1% for 3TC over the full concentration ranges. A cross-validation study demonstrated that the LC-MS/MS method afforded equivalent results to established methods consisting of a radioimmuno-assay for AZT and an HPLC-UV method for 3TC. Moreover, the LC-MS/MS was more sensitive, allowed markedly higher-throughput, and required smaller sample volumes (for 3TC only). The validated method has been used to support post-marketing clinical studies for Combivir a combination tablet containing AZT and 3TC.

The effects of age and body weight on anthropometric estimations of minimal wrestling weight in high school wrestlers.

The purpose of this study was to determine the effects of age and body weight on anthropometric estimations of minimal wrestling weight (MWW) in high school wrestlers. Five hundred and twenty-two high school wrestlers (M age +/- SD = 16.45 +/- 1.03 years) volunteered as subjects for this study. The total sample (N = 522) was dichotomized by age (less than 16 years, n = 171; greater than or equal to 16 years, n = 351) and body weight (less than or equal to 62.60 kg, n = 252; greater than 62.60 kg, n = 270). Cross-validation analyses included examination of the constant error (CE), standard error of estimate (SEE), r, and total error (TE). The results indicated that the quadratic skinfold equation of Lohman (EQ1; Table 2) most accurately estimated MWW in each group. Furthermore, it was recommended that MWW be calculated from EQ1 using the conversion constants of Lohman ([(5.03/BD)-4.59] x 100) to estimate relative fat from body density.

Validity of anthropometric estimations of body composition in high school wrestlers.

The purpose of this study was to determine the validity of 23 anthropometric equations for estimating body composition and minimal wrestling weight in high school wrestlers. A total of 409 high school wrestlers (M age +/- SD = 16.42 +/- 1.03 yrs) volunteered for this study. Twenty-three anthropometric measures including eight skinfolds, nine circumferences, and six diameters were obtained from each subject. The mean body density, determined from underwater weighing, was 1.0748 +/- 0.0100 g.cm-3. The crossvalidation analyses indicated that the quadratic skinfold equation of Lohman resulted in the most accurate estimation of body density. The total error, constant error, standard error of estimate, and r for this equation were 0.0077 g.cm-3, -0.0003 g.cm-3, 0.0076 g.cm-3 and .65, respectively. Furthermore, the results of this investigation indicated that the minimal wrestling weight equations of Tcheng and Tipton resulted in total error values (5.54 to 6.06 kg) which were too large to be of practical use for high school wrestlers.

Validity and intertester error of anthropometric estimations of body density.

The purposes of this study were to evaluate the intertester error in anthropometry and its effect on estimations of actual body density (ABD), as well as to assess the validity of predicted body density (PBD) from selected anthropometric equation. Sixteen adult males X +/- SD = 21.98 +/- 1.65 years) volunteered to be measured by three experienced and reliable testers at eight circumference, two diameter, and eight skinfold sites as well as by underwater weighing. Eighteen commonly used anthropometric equations which predict body density were selected for evaluation. Repeated measures ANOVA with Tukey post-hoc comparisons were used to determine differences between testers for each anthropometric measure and the PBD values, as well as between the PBD values and ABD. There were significant (p less than 0.01) differences between testers for all anthropometric measures except bi-iliac diameter and hips circumference. However, the magnitude of the differences between testers were considered to be quite small and of no practical concern. There were significant differences among the PBD values and ABD for all equations except the base 10 logarithmic transformations of Durnin and Womersley. Seven of the equations, however, resulted in a constant error of less than or equal to 0.0085 g.ml-1. Overall, there was no single anthropometric equation which satisfied all of the cross-validation criteria suggested by previous investigators, however the linear equation of Forsyth and Sinning which included the scapular and abdominal skinfolds, as well as the bitrochanteric diameter resulted in an extremely small constant error (0.0024 g.ml-1) and an appropriate standard deviation of the PBD values (0.0127 g.ml-1).

Isokinetic strength and body composition of high school wrestlers across age.

The purpose of this study was to determine the changes in body composition as well as absolute and relative isokinetic forearm and leg strength of high school wrestlers across age. One hundred ninety-five wrestlers (means age +/- SD = 16.36 +/- 1.12 yr) volunteered to be measured for strength using a Cybex II dynamometer at 30, 180, and 300 degrees.s-1. In addition, underwater weighing was used to determine body composition characteristics. The subjects were divided into four age groups: group 1 (G1) = 14.17-15.00 yr (N = 20); group 2 (G2) = 15.01-16.00 yr (N = 60); group 3 (G3) = 16.01-17.00 yr (N = 52); and group 4 (G4) = 17.01-18.50 yr (N = 63). One-way ANOVA or ANCOVA with Tukey post hoc comparisons indicated significant (P less than 0.05) changes across age for height, body weight, and fat-free weight, as well as absolute and relative forearm and leg strength. The results of this study indicated that, while a large portion of the improvements in strength across age were associated with increases in lean tissue, there was an additional "age effect" which could not be accounted for by changes in fat-free weight. Although the mechanism responsible for the "age effect" is unclear, it is possible that neural development contributed to the strength increases across age.