RESUMO
The isolation, partial identification and quantitative determination of four guaiphenesin glucuronides in plasma and urine of the horse is described. The identity of the glucuronides was checked by UV and fluorescence spectrophotometry, by NMR spectrometry and by mass spectrometry after permethylation. The applicability of the procedure to pharmacokinetic studies is demonstrated.
Assuntos
Glucuronatos/sangue , Guaifenesina/sangue , Concentração de Íons de Hidrogênio , Animais , Cromatografia Líquida de Alta Pressão/métodos , Glucuronatos/urina , Guaifenesina/urina , Cavalos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Espectrometria de Fluorescência/métodos , Espectrofotometria Ultravioleta/métodosRESUMO
1. After administration of styrene to rat, phenaceturic acid, a new metabolite, was isolated. After administration of d8-styrene to rat, deuterium-labelled phenaceturic acid was excreted in the urine. 2. Two metabolic pathways are proposed, based on mass spectra of the deuterium-labelled phenaceturic acid. 3. After a single dose of styrene (250 mg/kg), the excreted phenaceturic acid originating from styrene in the urine amounted to 1.4% dose.
Assuntos
Glicina/análogos & derivados , Estirenos/metabolismo , Animais , Cromatografia Gasosa , Cromatografia em Camada Fina , Feminino , Glicina/urina , Fenilacetatos/urina , RatosRESUMO
1. After administration of styrene (1) to rat, three sulphur-containing urinary metabolites were isolated in the molar ratio of III : IV : V equal to 65 : 34 : 1. 2. These metabolites were identified as N-acetyl-S-(1-phenyl-2-hydroxyethyl)cysteine (III), N-acetyl-S-(2-phenyl-2-hydroxyethyl)cysteine (IV) and N-acetyl-S-(phenacyl)cysteine (V). 3. After a single dose (250 mg/kg, 2.4 mmol/kg) styrene, the totally excreted mercapturic acids in the urine amounted to 10.7 +/- 1.0% (n = 5) of the dose.
