Assuntos
Transplante de Células-Tronco Hematopoéticas/métodos , Sobrecarga de Ferro/terapia , Flebotomia/métodos , Estudos de Coortes , Estudos de Viabilidade , Humanos , Sobrecarga de Ferro/sangue , Sobrecarga de Ferro/diagnóstico , Sobrecarga de Ferro/etiologia , Estudos Prospectivos , Condicionamento Pré-Transplante/métodosAssuntos
Doenças do Sistema Nervoso Central/etiologia , Encefalite/etiologia , Doença Enxerto-Hospedeiro/etiologia , Adulto , Doenças do Sistema Nervoso Central/diagnóstico , Doença Crônica , Encefalite/diagnóstico , Doença Enxerto-Hospedeiro/diagnóstico , Granuloma/diagnóstico , Granuloma/etiologia , Humanos , Linfoma Folicular/terapia , Masculino , Transplante de Células-Tronco/efeitos adversos , Transplante HomólogoRESUMO
Two nutritionally mutant strains of Renibacterium salmoninarum (Rs) were isolated that grew on tryticase soy agar (Rs TSA1) or brain heart infusion agar (Rs BHI1). These 2 strains could be continuously cultured on these media, whereas typical R. salmoninarum would only grow on KDM-2 agar. We determined no other phenotypic difference that could be used to distinguish them from wild-type R. salmoninarum. Both strains were found to be avirulent when 5 x 10(6) bacteria were intraperitoneally (i.p.) injected into Atlantic salmon. Rs TSA1, Rs BHI1, and Rs MT-239 (a R. salmoninarum strain previously shown to be attenuated) were tested as live vaccines in 2 separate trials. The best protection was seen with Rs TSA1. Vaccinated Atlantic salmon had relative percent survival (RPS) of 50 at 74 d post-challenge in Trial 1 and 76 at 60 d post-challenge in Trial 2. In both trials, 100% of the control salmon died from bacterial kidney disease (BKD) (within 40 d for Trial 1 and 50 d for Trial 2) after i.p. challenge with 5 x 10(6) live cells of the virulent isolate Rs Margaree.
Assuntos
Vacinas Bacterianas , Doenças dos Peixes/prevenção & controle , Infecções por Bactérias Gram-Positivas/veterinária , Micrococcaceae/imunologia , Vacinas Atenuadas , Animais , Infecções por Bactérias Gram-Positivas/prevenção & controle , Salmo salar , Vacinação/veterináriaRESUMO
Aeromonas salmonicida strains phenotypically differing in their A-layer, lipopolysaccharide, and macrophage cytotoxicity were tested in vitro for survivability in brook trout (Salvelinus fontinalis) serum with or without antibodies, and in vivo following intraperitoneal injection. The ability of brook trout peritoneal macrophages to phagocytize and kill the different phenotypes was investigated in an in vitro assay. The virulent strain, A. salmonicida 80204, which has the full complement of known virulence factors, as well as the recently described macrophage cytotoxin, was resistant in vitro to both the bactericidal activity of normal and immune serum, and to brook trout peritoneal macrophages. A. salmonicida SS-70.1, which possesses the A-layer but lacks the cytotoxin, was resistant to the bactericidal activity of normal and immune serum but was avirulent and killed by macrophages. Phenotypes lacking the A-layer, regardless of whether or not they possessed the macrophage cytotoxin were avirulent, susceptible to normal and immune serum and the bactericidal activity of peritoneal macrophages. A. salmonicida virulence expression requires both the A-layer and the macrophage cytotoxin.