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Objective In this study ,we constructed a series of recombinant plasmids carriers expressing shRNA targeting hTERT and Bi‐1 gene .These recombinant plasmids carriers were transfected into CNE‐2Z cell lines using Lip and continuously in‐duced the expression of shRNAs .Furthermore ,the shRNAs caused the degradation of mRNAs homologous in sequence with the target genes ,which lead to a sequence‐specific gene silencing .Methods The CNE‐2Z cells was divided into untreated group ,pEG‐FP‐N1 group and pEGFP‐N1/Lip group .Flow cytometry(FCM ) was applied to determine the transfection efficiency .The changes of hTERT and Bi‐1 gene expression were detected by Real‐time RT‐PCR and Western blotting .Results The best transfection effi‐ciency between plasmid and Lip was 2 .5 μg plasmid and 6 .25μL Lip .Conclusion We constructed several shRNA recombinant eu‐karyotic expression plasmids successfully .The recombinant plasmid can inhibit the expression of hTERT and Bi‐1 gene specifically and effectively .
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AIM: To investigate the clinical use and effect of a removable obturator made of elastic bionic materials in treating food impaction.METHODS: The removable obturator made of elastic denture materials were applied to 27 patients who suffered either mixed or horizontal food impaction and were admitted in Guilin Stomatological Hospital from October 2006 to April 2007. At weeks 4 and 12 following treatment, all patients were surveyed by questionnaires to investigate wearing comforts, and the histocompatibility of the obturator was simultaneously evaluated. Periodontal indexes were recorded before treatment, at weeks 4 and 12 after treatment. X-ray radiography was also performed before treatment and 12 weeks after treatment to compare alveolar condition.RESULTS: All the 27 patients were involved in the result analysis. They were all satisfied with the obturator after twelve weeks treatment, and the questionnaire showed 96.3%-100% satisfactions from the patients. The measuring results of periodontal indexes at different periods of the treatment showed no statistical significance. There were no remarkable changes in the tooth mobility and X-ray image analysis before treatment and 12 weeks after treatment.CONCLUSION: With good histocompatibility, the removable obturator made of elastic denture materials has been proved to be well histocompatible, acceptable and effective for the patients who suffer from food impaction in the short term.
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Objective:To observe the effect of overdose fluoride on the expression of bFGF in rat's dental pulps.Methods:NaF at 20 mg/(kg?d) was given to each of 10 Wister rats by stomach perfusion for 8 weeks, another 10 rats were given the same volume of distilled water as the controls.After treatment,HE staining was used to observe the morphology of the teeth, immunohistochemical staining was adopted for study the expression of bFGF in the dental pulps of the rats.Results:The enamel layer and dentin layer of the incisors in NaF treated rats were thinner than those in the controls,the interglobular dentin was increased and the expression of bFGF in dental pulp and inner dentin was inhibited in the NaF treated rats(P
