RESUMO
OBJECTIVE: To study the biocompatibility of the polylactic acid (PLA)-absorbable root post film prepared by solvent evaporation film. METHODS: The PLA post film-leaching liquor was prepared by the solvent evaporation of PLA root post films, and its biocompatibility was measured. Sources of human gingival fibroblasts (HGF) were cultivated in vitro and identified initially by the immunohistochemistry method. The toxicity reaction, survival rate, and morphological change of HGF incubated in PLA post film-leaching liquor were observed and tested by morphological observation, 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay, and flow cytometry. The HGF were implanted on the PLA post film and observed by 4',6-diamidino-2-phenylindole staining. After implanting the PLA post film, the surrounding subcutaneous tissue of SD rats and the implants were removed from the back of the rats in the 1st, 4th, 8th, and 12th weeks to observe the general condition of the incisions. The organization was given the hematoxylin-eosin (HE) stain to find the organization condition of local tissue inflammation and foreign body reaction through the infiltration degree of inflammatory fibroblasts. RESULTS: The effects of the PLA post film-leaching liquor on the proliferation of HGF and fibroblast toxicity were insignificant. The living fibroblast rate was similar to the normal control group. The HGF were able to grow on the PLA film. The results of the organization general observation and HE staining of the rats were similar to the results for the control group, which met all the demands made by biological safety. CONCLUSIONS: The PLA-absorbable root post film prepared by the solvent evaporation film has good biocompatibility and can be used for further clinical research.
