[Estrogenic activity of some lucerne varieties]. / Vurkhu estrogennati aktivnost na niakoi sortove liutserna.

Studies was the estrogenic activity of lucerne--the local population Dounavka, the zonated French variety Du Puits, the promising Bulgarian variety. Pleven 1, the population Boinitsa, and the French variety Du Poitou. The estrogenic activity was determined by the biologic method based on the increase in the uterus weight of infantile mice after treatment with estrogens. Paper chromatography tests were also carried out to establish the presence of genestein and cumestrol. The study comprised three consecutive years, the extent of estrogenic activity being dependent on the amount of annual precipitations and the yields of green mass and dry matter. The study revealed that there were variations in the estrogenic activity of the investigated varieties and populations of lucerne within a wide range depending on the year of use and the green mass and dry matter obtained. High was it in the local population Dounavka, the French variety Du Poitou, and the Bulgarian variety Pleven 1. Comparatively lower was the estrogenic activity in Du Puits, and the local population Boinitsa. In all varieties and populations studied genestein was found in the first swath, and cumestrol--in the second swath. The remaining swaths showed differences in the content of genestein and cumestrol in the individual varieties and populations.

[Effect of several factors on the estrogen activity of lucerne]. / Vliianie na niakoi faktori vurkhu estrogennata aktivnost na liutsenata

Investigations were carried out on the estrogen activity of lucerne from various mowings harvested in years of different climate, studying the effect of age and yield of the crop. The observations were made during three successive years on local populations of lucerne grown under nonirrigation conditions. The estrogen activity was determined ny Bikov's biologic method, and the presence of cumestrol and genestein was established through chromatography. It was found that the estrogen activity of lucerne was to a large extent dependent on the intensity of growth. Young plants giving higher yield of green mass and dry matter manifested higher estrogen activity. Highest was the activity of lucerne from the first mowing, gradually decreasing in each of the following mowings. Variations were likewise established in the content of genestein and cumestrol in dependence on the mowing itself and the yield. All samples demonstrated that genestein was present in the first and fourth mowing, while the content of cumestrol varied within a wide range showing no markedly expressed correlations. The activity of the local lucerne populations during the first years of using the crop and the first mowings of green mass was shown to bring about disturbances in the reproduction process of ruminant animals provided lucerne is offered for a long time in greater amounts.

[Effect of a gestagen used for the induction of synchronous estrus and fertilization of cattle]. / V'rkhu efekta na edin gestagen, izpolzvan za indutsirane na sinkhronen estrus i zaplozhdane pri iunitsi

The preparations Synchrosyn, Gestafortin, and Bovisynchron, having chlormadinonacetate as a common active ingredient, were used to induce synchronous estrus and fertilization in heifers. A total of 254 animals were involved belonging to the following breeds: Bulgarian Simmental, Bulgarian Red, Bulgarian Brown, and Black Pied. All three preparations were administered orally: Synchrosyn and Gestafortin were given with the forage at the rate of 10 mg chlormadinonacetate, and Bovisynchron was offered twice daily at an interval of 12 hours, rated 1 cu. cm each time, by means of a syringe (a daily dose of 20 mg clormadinonacetate).