RESUMO
The simple mathematical model based on the stoichiometric structure of carbohydrate metabolism and the only allosteric regulation presented, i. e. activation of phosphofructokinase by AMP, was used to study the mechanism of the Pasteur effect, e. g. interrelationship of glycolysis, the Krebs cycle and H-transporting shuttles at varying rates of oxidative phosphorylation and ATPase load. It was shown that the mechanism of the Pasteur effect is based on the presence of two negative feed-back mechanisms in carbohydrate metabolism, namely by the level of ATP in glycolysis and by the level of mitochondrial NADH in the Krebs cycle and H-transporting shuttles. It was also shown that the value and sign of the Pasteur effect depend on the level of ATPase load. The role of this phenomenon in stabilization of ATP in the cell is discussed. The effects of changes in the allosteric properties of phosphofructokinase and low activity of H-transporting shuttles on the Pasteur effect was studied. It was shown that the low values of the pasteur effect in tumour tissues are mainly determined by an insufficient activity of oxidative phosphorylation.
Assuntos
Metabolismo dos Carboidratos , Ciclo do Ácido Cítrico , Metabolismo Energético , Glicólise , Adenosina Trifosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Transporte Biológico Ativo , Concentração de Íons de Hidrogênio , Cinética , Matemática , Mitocôndrias/metabolismo , Modelos Biológicos , NAD/metabolismo , Oxirredução , Fosforilação OxidativaRESUMO
A simple mathematical model for carbohydrate energy metabolism based on the stoichiometic structure of glycolysis, the Krebs cycle and oxidative phosphorylation is proposed. The only allosteric regulation involved in the model is phosphofructokinase activation by AMP. Simple as it is, the model can explain the following properties of carbohydrate metabolism: a drastic rise of the rate of glucose consumption during transition to a higher level of ATPase load; stabilization of ATP and an increase of the steady state rates of glycolysis and oxidation of cytoplasmic NADH by the H-transporting shuttles and of pyruvate in the Krebs cycle with increasing rate of the ATPase load; activation of glycolysis and a decrease of the rate of oxidative phosphorylation following an inhibition of the H-transporting shuttles. The mechanisms of the coordinated changes in the steady state rates of glycolysis, the H-transporting shuttles and the Krebs cycle at varying ATPase load in the cell are discussed.