Synchronous analysis of acetaminophen, codeine, and caffeine in human fluids employing graphite screen-printed electrodes.

A facile electrochemical approach is proposed for the synchronous determination of acetaminophen (ACP), codeine (COD) and caffeine (CAF) utilizing unmodified screen-printed electrodes (SPEs). The determination of ACP, COD and CAF has been explored across different supporting electrolytes including sulfuric acid (H2SO4), hydrochloric acid (HCl), phosphoric acid (H3PO4) and Briton Robinson (B.R) buffer solutions. It was found that a 0.05 mol L-1 sulfuric acid solution is an optimal supporting electrolyte utilized for voltammetric analysis of ACP, COD, and CAF with improved sensitivity, stability, and reproducibility. The electro-analytical sensing of ACP, COD and CAF was investigated using SPEs within linear concentration ranges of 3.0-35.0 µmol L-1, 10-160 µmol L-1 and 10-160 µmol L-1 and revealed competitively low limits of detection (3S/N) of 0.9, 4.8 and 6.3 µmol L-1 for ACP, COD and CAF, respectively. The results indicated the possibility of such a simple and quick electroanalytical protocol for online monitoring of pharmaceutical formulations comprising ACP, COD, and CAF drugs in human fluids with satisfactory recovery.

Enhancing the Conductivity and Dielectric Characteristics of Bismuth Oxyiodide via Activated Carbon Doping.

Activated carbon/BiOI nanocomposites were successfully synthesized through a simplistic method. The produced composites were then characterized using XRD, TEM, SEM-EDX, and XPS. The results showed that BiOI with a tetragonal crystal structure had been formed. The interaction between activated carbon and BiOI was confirmed via all the mentioned tools. The obtained nanocomposites' electrical conductivity, dielectric properties, and Ac impedance were studied at 59 KHz-1.29 MHz. AC and dc conductivities were studied at temperatures between 303 and 573 K within the frequency range of 59 KHz-1.29 MHz. The 10% activated carbon/BiOI nanocomposite possessed dc and AC conductivity values of 5.56 × 10-4 and 2.86 × 10-4 Ω-1.cm-1, respectively, which were higher than BiOI and the other nanocomposites. Every sample exhibited increased electrical conductivity values as the temperature and frequency rose, suggesting that all samples had semiconducting behavior. The loss and dielectric constants (ε' and ε″) also dropped as the frequency increased, leading to higher dielectric loss. The Nyquist plot unraveled single semicircle arcs and a decreased bulk resistance, indicating decreased grain boundary resistance. Consequently, the electrical characteristics of BiOI, 1C/BiOI, 5C/BiOI, and 10C/BiOI implied their applicability as dielectric absorbers, charge-stored capacitors, and high-frequency microwave devices.

Use of benzothiophene ring to improve the photovoltaic efficacy of cyanopyridinone-based organic chromophores: a DFT study.

The benzothiophene based chromophores (A1D1-A1D5) with A-π-A configuration were designed via end-capped tailoring with benzothiophene type acceptors using reference compound (A1R). Quantum chemical calculations were accomplished at M06/6-311G(d,p) level to probe optoelectronic and photophysical properties of designed chromophores. Therefore, frontier molecular orbitals (FMOs), binding energy (Eb), open circuit voltage (Voc), transition density matrix (TDM), density of state (DOS) and UV-Vis analyses of A1R and A1D1-A1D5 were accomplished. The designed compounds (A1D1-A1D5) exhibited absorption values in the visible region as 616.316-649.676 nm and 639.753-665.508 nm in gas and chloroform phase, respectively, comparing with reference chromophore. An efficient charge transference from HOMO towards LUMO was found in A1D1-A1D5 chromophores which was further supported by TDM and DOS analyses. Among all chromophores, A1D2 exhibited unique characteristics such as reduced band gap (2.354 eV), higher softness (σ = 0.424 eV), lower exciton binding energy (0.491 eV) and maximum value of open circuit voltage (Voc = 1.981 V). Consequently, A1D2 may be considered as potential candidate for the development of optoelectronic devices. These analyses revealed that the studied compounds exhibited promising findings. They may be utilized in the realm of organic solar cells.

The effect of butyric acid and nucleotides supplementation on broiler (Gallus gallus domesticus) growth performance, immune status, intestinal histology, and serum parameters.

Background: Butyric acid and its derivatives support the immune system, lessen inflammation, and lessen oxidative stress in broilers in addition to preserving gut homeostasis and epithelial integrity. Broiler performance has also been demonstrated to rise with the addition of nucleotides to the diet. Aim: The purpose of the study was to ascertain the effects of butyric acid and nucleotides added to feed on the overall performance, immunity, oxidant/antioxidant enzyme levels, intestinal histology, and hepatic functions of broilers. Methods: Four experimental groups of thirty chickens, each were used in the present study. The groups were assigned as a control group that received normal diet without additives, butyrate (B) group received the diet supplemented with butyric acid (250 g/ton feed), nucleotides (N) group received the diet supplemented with nucleotides (200 g/ton feed), and the fourth group received the diet supplemented with a combination of butyrate and nucleotide (BN) (250 g/ton B feed, and 200 g/ton N feed, respectively). Necrotic enteritis was produced in ten birds from each group to assess the immune-modulatory effect of these supplements, antioxidant status, intestinal histology, and liver functions were measured in all experimental groups. Results: The addition of butyric acid and nucleotides to feed enhanced body weight, growth performance, hepatic functions, and antioxidant capabilities. Histological sections of the gut from challenged or unchallenged (with necrotic enteritis) groups in the BN group showed considerable improvement, as shown by strong proliferation in intestinal crypts and villus enterocytes. Conclusion: Nucleotides and butyric acid can be added to broiler feeding regimens to enhance growth and health.

Cervical Myelopathy with Concomitant Guillain-Barré Syndrome: A Case Report.

CASE: We present a case of a 67-year-old female patient with concomitant cervical spondylotic myelopathy (CSM) and Guillain-Barré syndrome (GBS). Surgical intervention, including cervical decompression and arthrodesis, was performed to address cervical myelopathy symptoms. Despite initial improvement, the patient's motor function deteriorated, leading to the diagnosis of GBS. The patient's hospital course was protracted with a complicated recovery. CONCLUSION: This case emphasizes the clinical details of coexisting CSM and GBS, highlighting the importance of diagnosing and considering demyelinating diseases when determining the optimal timeline for surgical intervention. These findings inform decision-making for clinicians encountering similar patient presentations.

Computational Foretelling and Experimental Implementation of the Performance of Polyacrylic Acid and Polyacrylamide Polymers as Eco-Friendly Corrosion Inhibitors for Copper in Nitric Acid.

Copper is primarily used in many industrial processes, but like many other metals, it suffers from corrosion damage. Polymers are not only one of the effective corrosion inhibitors but also are environmentally friendly agents in doing so. Hence, in this paper, the efficacy of two polyelectrolyte polymers, namely poly(acrylic acid) (PAA) and polyacrylamide (PAM), as corrosion inhibitors for copper in molar nitric acid medium was explored. Chemical, electrochemical, and microscopic tools were employed in this investigation. The weight-loss study revealed that the computed inhibition efficiencies (% IEs) of both PAA and PAM increased with their concentrations but diminished with increasing HNO3 concentration and temperature. The results revealed that, at similar concentrations, the values of % IEs of PAM are slightly higher than those recorded for PAA, where these values at 298 K reached 88% and 84% in the presence of a 250 mg/L of PAM and PAA, respectively. The prominent IE% values for the tested polymers are due to their strong adsorption on the Cu surface and follow the Langmuir adsorption isoform. Thermodynamic and kinetic parameters were also calculated and discussed. The kinetics of corrosion inhibition by PAA and PAM showed a negative first-order process. The results showed also that the used polymers played as mixed-kind inhibitors with anodic priority. The mechanisms of copper corrosion in nitric acid medium and its inhibition by the tested polymers were discussed. DFT calculations and molecular dynamic (MD) modelling were used to investigate the effect of PAA and PAM molecular configuration on their anti-corrosion behavior. The results indicated that the experimental and computational study are highly consistent.

Black seed and rosemary nanoformulations as green insecticides for the granary weevil, Sitophilus granarius (L.) (Coleoptera: Curculionidae).

The development of nano-insecticides has attracted much interest in the last decade because it has the potential to result in an alternative pest-management strategy and also reduce the risk of chemically based insecticides. Herein, native rosemary (Rosmarinus officinalis) and black seed (Nigella sativa) oils were utilized for preparing their nanoemulsions by spontaneous emulsification method in the presence of tween 80 as a structure-directing agent. The prepared nanoemulsions were explored for granary weevil, Sitophilus granarius (L.), adults control and compared with their oils. Within the typical droplet sizes of 100 and 224 nm, both rosemary and black seed nanoemulsions were found to be physically and thermodynamically stable. The insecticidal activity of the nanoemulsions was higher than that of the crude oils. After 24 h of exposure, the LD50 values of rosemary and black seed nanoemulsions were estimated to be 102.56 and 35.08 µg/g, respectively, compared to 188.95 and 210.09 µg/g of their oils. These results revealed that the droplet size and chemical composition of the nanoemulsion are the significant factors that affect their toxicity. Surprisingly, the nanoemulsions had no effect on seed germination at LD50 or even LD99 . The utilization of such nanoformulations might open up a new avenue for ecofriendly pest control that is not damaging to humans or the environment, as well as a growing agricultural economy. PRACTICAL APPLICATION: The loss of cereals in postharvesting is one of the major challenges in the world because up to 50% of harvested grains might be destroyed. The intensive usage of chemicals caused harmful effects on humans and the environment. Thus, we prepared rosemary and black seed nanoemulsions and applied them for the grain weevil, S. granarius, control. The results showed superior toxicological efficacy without any effects on seed germination compared with their native oils. Such green strategy could be used instead of chemical insecticides to be environmentally safe for animals and humans.

Transcriptome comparisons detect new genes associated with apoptosis of cattle and buffaloes preantral follicles.

BACKGROUND: To develop new breeding technology to improve the breeding ability of bovine, it is the development trend to find the main reason for the occurrence of atresia in these organisms. Transcriptomes of small (100-120 µm) and large (200-220 µm) preantral follicles from cattle and buffalo ovaries were evaluated in vivo and in vitro to understand the transcriptional modulation in preantral follicles that leads to the phenomenon of atresia. METHODS: The preantral follicles were checked as dead, damage, or live follicles in vivo and in vitro by using trypan blue then bisbenzimide and propidium iodine. Transcriptomes of small (100-120 µm) and large (200-220 µm) preantral follicles of cattle and buffalo were evaluated in vivo and in vitro by microarray and RT-PCR. Healthy preantral follicles were selected based on staining results, and then RNA was extracted from them. RESULTS: The viability percentage of preantral follicles in cattle was higher (26.7% and 20%) than buffalo (10%) in vivo and in vitro, respectively. According to the microarray data analysis for cattle preantral follicles, only eleven genes were detected corresponding to five upregulated and six downregulated in large size (200-220 µm) compared to small (100-120 µm) size preantral follicles, while in buffalo, 171 genes were detected (92 upregulated and 79 downregulated) in large size compared to small preantral follicle size. The results of RT-PCR of the selected genes (FASTKD1, BAG2, RHOB, AGTR2, MEF2C, BCL10, G2E3, TM2D1, IGF-I, IGFBP3, PRDX3, and TRIAP1) validated the microarray results. In conclusion, the data of gene expression showed significant differences between small and large sizes in both buffalo and cattle preantral follicles. CONCLUSION: Apoptotic genes were upregulated in the large preantral follicle compared with the small preantral follicles. Moreover, the expression level of these apoptotic genes was significantly upregulated in buffalo than in the cattle. Most of these genes were significantly upregulated in the large buffalo preantral follicle compared with the small size. However, anti-apoptotic genes were upregulated in large cattle preantral follicle and downregulated in large buffalo preantral follicle.

Salvia officinalis Extract and 17ß-Estradiol Suppresses Ovariectomy Induced Osteoporosis in Female Rats.

<b>Background and Objective:</b> Osteoporosis is a progressive metabolic disorder characterized by an impaired bone formation that leads to increased morbidity and mortality.<i> Salvia officinalis </i>is a source of phytoestrogens that could help mitigate the risk of osteoporotic rat fracture by exerting sex hormones. Therefore, the present study was designed to investigate the curative effect of <i>Salvia officinalis </i>Extract<i> </i>(SOE) and<i> </i>17ß-estradiol (E₂) and their combination<i> </i>on bone loss in female rats with ovariectomy-induced estrogen deficiency <b>Materials and Methods:</b> Forty adult female albino rats were divided into five groups, which included Sham control (Sham), ovariectomy (OVX), OVX+SOE, OVX+E₂ and OVX +SOE+E₂.<i> </i>SOE (10 mL kg¹) and E₂ (30 µg kg¹) had been daily gavaged in the OVX+SOE, OVX+E₂ and OVX+SOE+E₂, respectively for 6-weeks. <b>Results:</b> The model of ovariectomy resulted in osteoporosis as demonstrated by the decreased serum Ca, P, vitamin D, E₂ level associated with a significant increase in PTH levels in comparison to the sham control group. Besides, OVX to rats caused up-regulation in the levels of CTX-1, P1NP, BALP, OC and RANKL comparable to the sham control group. Moreover, SOE and E₂ significantly modulated the calciotropic parameters and improved all bone turnover markers as well as RANKL as compared to the OVX group. However, Histopathological and immunohistochemical results showed defective mineralization with the destruction of the bone matrix and increased TNF-α expression from the OVX group relative to the treated groups. <b>Conclusion:</b> These results suggest that both SOE and E₂ or their combined administration are efficient inhibitors against ovariectomy-induced bone loss in female rats.

Sea urchin-like calcium borate microspheres and synergistic action with cholinesterase-inhibiting insecticides for ecofriendly Spodoptera littoralis control.

The development of nanoagrochemicals has attracted much attention in the last decade to overcome the recent agricultural and environmental challenges associated with the intensive usage of insecticides. Herein, nanostructured calcium borate materials with hierarchical sea urchin-like microspheres and microblocks have been synthesized by a facile hydrothermal method. The insecticidal activity of CaB2O4 and its synergistic combination with cholinesterase-inhibiting insecticides are explored against Spodoptera littoralis (S. littoralis) for the first time via a feeding bioassay protocol. The insecticidal efficacy of sea urchin-like microspheres (CB-A) is estimated to be LC50 = 207 mg L-1 which is two-fold higher than that of microblocks (CBM-A) with LC50 = 406 mg L-1 after eleven days of exposure. The synergistic combination of the CB-A sample with methomyl and chlorpyrifos increases the toxicity to 2.4 and 2.6-fold higher than that of the individual insecticides, respectively. Significantly, sea urchin-like CaB2O4 microspheres cause physical damage to the external insect's cuticle layer, which consequently enhances the uptake of organic insecticides. Our results revealed that calcium borate micro-/nano-structures can be employed as a multifunctional nanoagrochemical in various agricultural programs for S. littoralis control and decrease the usage of cholinesterase-inhibiting insecticides.

Synthesis of highly crystalline LaFeO3 nanospheres for phenoxazinone synthase mimicking activity.

LaFeO3 nanospheres with an orthorhombic perovskite structure were synthesized by a sol-gel autocombustion method in the presence of different citric acid ratios (x = 2, 4, 8, and 16) and utilized for the photocatalytic conversion of o-aminophenol (OAP) to 2-aminophenoxazine-3-one (APX) for the first time. OAP is one of the most toxic phenolic derivatives used as a starting material in many industries; however, the dimerization product APX has diverse therapeutic properties. Photocatalytic conversion was carried out in ethanol/water and acetonitrile/water mixtures in the absence and presence of molecular oxygen at ambient temperature via the oxidative coupling reaction that mimics phenoxazinone synthase-like activity. The LaFeO3 samples showed a superior photocatalytic activity of OAP to APX with rate constants of 0.43 and 0.92 min-1 in the absence and presence of molecular oxygen, respectively. Thus, the LaFeO3 nanozymes could be used as promising candidates in industrial water treatment and phenoxazinone synthase-like activity.

Nanotechnology improves the therapeutic efficacy of gemcitabine against a human hepatocellular carcinoma cell line and minimizes its in vivo side effects.

Nanotechnology has become a promising approach for addressing cancer therapy limitations because it reduces side effects and increases the efficacy of antineoplastic agents. Therefore, this research was designed to compare the in vitro therapeutic efficacy and in vivo adverse effects of gemcitabine (GEM) and gemcitabine-loaded silver nanoparticles (GEM-AgNPs). GEM molecules were successfully attached to AgNP surfaces with a homogenous and spherical shape. The zeta size of AgNPs and GEM-AgNPs was 79.35 ± 3.2 and 75.1 ± 7 nm, respectively. The anticancer effect of AgNPs and GEM-AgNPs was investigated against a human hepatocellular carcinoma cell line (HepG2), and cytotoxic activity was evaluated by MTT assay. Apoptosis/necrosis and cell cycle arrest were also assessed. The cytotoxic activity was recorded in a concentration-dependent way. The findings have shown that GEM-AgNPs induced a better cytotoxic effect with an IC50 value of 13.63 µg/mL compared to GEM (IC50 value of 24.19 µg/mL) or AgNPs alone (IC50 value of 50.6 µg/mL). GEM-AgNPs induced pre-G1 arrest and apoptotic/necrotic cell death. Our in vivo analysis involved the use of 40 male rats assigned equally into the control rats, and rats injected intraperitoneally with GEM (134 mg/kg), AgNPs (1 mg/kg), and GEM-AgNPs (134 mg/kg). GEM and GEM-AgNPs were administered on the 1st, 7th, and 14th day of the experiment. Intraperitoneal GEM injection induced marked hematological, biochemical, hepatorenal, and histopathological alterations, while the loading of GEM in AgNPs to some extent ameliorated these alterations and significantly improved its therapeutic efficacy against HepG2 cells. These findings indicate the potential use of GEM-AgNPs in the clinical setting for anticancer treatment.

A ten year analysis of maternal deaths in a tertiary hospital using the three delays model.

BACKGROUND: Reducing maternal mortality ratios (MMRs) remain an important public health issue in Egypt. The three delays model distinguished three phases of delay to be associated with maternal mortality: 1) first phase delay is delay in deciding to seek care; 2) second phase delay is delay in reaching health facilities; and 3) third phase delay is delay in receiving care in health facilities. Increased health services' coverage is thought to be associated with a paradigm shift from first and second phase delays to third phase delay as main factor contributing to MMR. This study aims to examine the contribution of the three delays in relation to maternal deaths. METHODS: During a 10 year period (2008-2017) 207 maternal deaths were identified in a tertiary hospital in Minia governorate, Egypt. Data were obtained through reviewing medical records and verbal autopsy for each case. Then data analysis was done in the context of the three delays model. RESULTS: From 2008 to 2017 MMR in this hospital was 186/100.000 live births. Most frequent causes of maternal mortality were postpartum hemorrhage, hypertensive disorders of pregnancy and sepsis. Third phase delay occurred in 184 deaths (88.9%), second phase delay was observed in 104 deaths (50%), always together with other phases of delay. First phase delay alone was observed in 13 deaths (6.3%) and in 82 deaths (40%) with other phases of delay. One fifth of the women had experienced all three phases of delay together. Major causes of third phase delay were delayed referral from district hospitals, non-availability of skilled staff, lack of blood transfusion facilities and shortage of drugs. CONCLUSIONS: There is a paradigm shift from first and second phases of delay to the third phase of delay as a major contributor to maternal mortality. Reduction of maternal mortality can be achieved through improving logistics, infrastructure and health care providers' training. TRIAL REGISTRATION: This study is a retrospective study registered locally and approved by the ethical committee of the Department of Obstetrics and Gynaecology, Minia University Hospital on 1/4/2016 (Registration number: MUEOB0002).

Malaria epidemiology in Kobeni department, southeastern Mauritania from 2015 to 2017.

BACKGROUND: Plasmodium falciparum malaria is endemic in the southern sahelian zone of Mauritania where intense internal and trans-border human and livestock movement occurs. The risk of importation and spread of drug-resistant parasites need to be regularly assessed in this region. The objective of the study was to assess the recent malaria situation near the Mauritania-Mali border. METHODS: Between February 2015 and December 2017, patients with fever or history of fever during the previous 48 h, presenting at the health centre of Kobeni city, were screened for malaria using a rapid diagnostic test (RDT) and microscopic examination of blood smears. The diagnosis was later confirmed by PCR. Cohen's kappa statistics was used to estimate the degree of agreement between diagnostic methods. Fisher's exact test was used to compare proportions. The odds ratio was calculated to measure the association between the use of bed nets and malaria infection. RESULTS: A total of 2326 febrile patients (mean age, 20.2 years) were screened for malaria. The presence of malaria parasites was detected by RDT and microscopy in 53.0% and 49.3% of febrile patients, respectively, and was confirmed by PCR in 59.7% (45 missing data). Of 1361 PCR-positive samples, 1205 (88.5%) were P. falciparum, 47 (3.5%) P. vivax, and 99 (7.3%) P. falciparum-P. vivax mixed infection. Malaria transmission occurred mostly during and shortly after the rainy season. The annual rainfall was relatively low in 2016 (267 mm) and 2017 (274 mm), compared to 2015 (448 mm), and coincided with a decline in malaria prevalence in 2016-2017. Although 71.8% of febrile patients reported to possess at least one bed net in the household in our questionnaire, its reported use was not protective against malaria infection (odds ratio: 1.1, 95% CI: 0.91-1.32). CONCLUSIONS: Our study confirmed that P. falciparum is the dominant species in the sahelian zone and that malaria transmission is seasonal and associated with rainfall in this zone. The application of the current national policy based on rapid and reliable malaria diagnosis, case management with artemisinin-based combination therapy, intermittent preventive treatment for pregnant women, distribution and use of long-lasting insecticide impregnated bed nets, and the planned introduction of seasonal malaria chemoprevention for all children under 6 years old is expected to sustainably reduce malaria transmission in this zone.

Single or combined protective and therapeutic impact of taurine and hesperidin on carbon tetrachloride-induced acute hepatic injury in rat.

Currently, hepatic injury due to environmental pollutants extremely threatens human health and elicits great concern. Hence, there is a high global interest to find natural novel formulation products with potent hepatoprotective activity to combat liver disease. Hence, we evaluated the protective or therapeutic effect of hesperidin (HSP) and taurine (TAU), individually and in combination, on carbon tetrachloride (CCl4)-induced acute hepatic injury in rats. The pre- or posttreatment by HSP and/or TAU significantly depressed CCl4-induced elevation of alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, gamma-glutamyl transferase, total bilirubin, direct bilirubin, indirect bilirubin, malondialdehyde, globulins (α1, α2, ß, and γ), albumin/globulin ratio, triglycerides, total cholesterol, high-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, low-density lipoprotein cholesterol, nitric oxide, and myeloperoxidase levels. Also, the pre- or posttreatment by HSP and/or TAU significantly minimized CCl4-induced reduction of superoxide dismutase, catalase, reduced glutathione, and albumin concentrations. Furthermore, the protective or therapeutic administration of HSP and/or TAU markedly restored the CCl4-induced altered hepatic architecture, depleted glycogen, and DNA contents. Notably, alleviating CCl4-induced hepatotoxicity was more prominent in the protective groups than the therapeutic groups. More importantly, most of biochemical and histopathological parameters of HSP+TAU did not significantly differ from those of separate TAU or HSP neither before nor after CCl4 exposure. Conclusively, HSP or TAU could be candidate protective agents against CCl4 hepatotoxic impacts but the combination of both bioactive offers only a limited synergistic effect. Graphical abstract.

A screen-printed electrochemical sensing platform surface modified with nanostructured ytterbium oxide nanoplates facilitating the electroanalytical sensing of the analgesic drugs acetaminophen and tramadol.

An electrochemical sensing platform based upon screen-printing electrodes (SPEs) modified with nanostructured lanthanide metal oxides facilitate the detection of the widely misused drugs acetaminophen (ACP) and tramadol (TRA). Among the metal oxides examined, Yb2O3 nanoplates (NPs) were found to give rise to an optimal electrochemical response. The electroanalysis of ACP and TRA individually, and within mixtures, was performed using cyclic and differential pulse voltammetry. The ACP and TRA exhibited non-overlapping voltammetric signals at voltages of +0.30 and + 0.67 V (vs. Ag/AgCl; pH 9) using Yb2O3-SPEs. Pharmaceutical dosage forms and spiked human fluids were analyzed in wide linear concentration ranges of 0.25-654 and 0.50-115 µmol.L-1 with limits of detection (LOD) of 55 and 87 nmol.L-1 for ACP and TRA, respectively. The Yb2O3-SPEs offer a sensitive and chemically stable enzyme-free electrochemical platform for ACP and TRA assay. Graphical abstractSchematic presentation of one-shot electrochemical analysis of misused drugs, tramadol (TRA) and acetaminophen (ACP) by utilizing ytterbium oxide nanoplates modified screen-printed electrodes (Yb2O3-SPEs). The Yb2O3-SPEs showed interesting responses for ACP and TRA within pharmaceutical formulations and human fluids.

Influence of preparation method on structural, optical, magnetic, and adsorption properties of nano-NiFe2O4.

Nickel ferrite (NiFe2O4) nanoparticles are prepared through different routes (microwave, co-precipitation, and pyrolysis) and tested for water purification applications through adsorption removal of an acid red dye B as a model organic pollutant. The characterizations of the prepared samples were done using XRD, FT-IR, SEM, TEM, BET, UV-Vis absorbance, Raman spectrum, and vibrating sample magnetometer (VSM). All samples showed an inverse spinel crystal structure. The obtained results pointed out to the effect of the synthetic route on the morphology, particle size, optical, and magnetic properties of the prepared ferrites. Magnetic measurements showed super-paramagnetic behavior for all samples. The magnetic saturation (Ms) of the sample prepared by pyrolysis, was found to possess the highest saturation value, 34.8 emu/g. Adsorption experiments were performed under the change in several parameters, such as pH, adsorbent dosage, and initial dye concentration. A dye removal percentage of 99% was reached under the optimum state. The isothermal adsorption of the acid red dye was investigated using several models, in which the experimental data could be best described by the Freundlich model. Several kinetic and equilibrium models were inspected by linear regression analysis and showed best fitting for the adsorption data through pseudo-second-order model. The calculated thermodynamic parameters indicated that the adsorption of acid red dye onto all the ferrite samples is a spontaneous and endothermic physical adsorption process.

Effect of acute gold nanorods on reproductive function in male albino rats: histological, morphometric, hormonal, and redox balance parameters.

In this study, we investigated the effect of acute administration of gold nanorods (AuNRs) on testicular function, sexual hormones, and oxidative stress parameters in male albino rats. Forty mature male albino rats were divided into two equal groups (n = 20/each). The first group received 1 ml saline solution intraperitoneally (i.p.). The second group received single i.p. injection of 75 µg 50 nm AuNRs/kg/bwt. Five rats from each group were sacrificed on days 1, 3, 7, and 14 post treatment and blood samples were collected for hormonal and biochemical analysis. Testes were collected from each group at each time point for histopathology, morphometric, and transmission electron microscope analyses of testis and epididymis. Results indicated that i.p. injection of AuNRs did not produce any histopathological changes. Morphometric analysis of testicular samples revealed that the height of lining epithelium was significantly (P < 0.05) higher in AuNR group on days 3 and 14 post treatment, and the minor axis of seminiferous tubules was higher (P < 0.05) in AuNR-injected rats than in control group. For the epididymis, the number of spermatozoa was significantly (P < 0.05) higher on days 7 and 14 after AuNR injection when compared with control rats. AuNRs were not detected by TEM at all time points of the experiment. Serum analysis demonstrated that total and free testosterone values significantly (P < 0.05) increased on days 1, 3, 7, and 14 post AuNR injection. LH was higher (P < 0.05) in AuNRs-injected rats on days 3, 7, and 14 post injection, while FSH values were higher (P < 0.05) in AuNR group on days 3 and 14. Malondialdehyde significantly (P < 0.05) decreased on days 3, 7, and 14 in AuNR group, while catalase, glutathione peroxidase, and superoxide dismutase values were significantly (P < 0.05) elevated on days 3, 7, and 14 in AuNRs-injected rats compared with control group. In conclusion, intraperitoneal injection of 50 nm AuNRs is safe on the reproductive function and has an antioxidant action.

Electrochemical determination of antihypertensive drugs by employing costless and portable unmodified screen-printed electrodes.

Hypertension increases the risk of heart disease and stroke, is commonly known as a silent killer disease and considered as one of the key risk factor for premature death and disability over the world. Herein, we report for the first time a sensitive, costless and reproducible voltammetric method for individual determination of five antihypertensive drugs namely, propranolol (PRO), timolol (TIM), amlodipine (AML), amiloride (AMI) and triamterene (TRI) using differential pulse voltammetry at bare/unmodified screen-printed carbon electrodes (SPEs) in presence of sodium dodecyl sulfate (SDS). Each drug exhibits an electrochemical signal in aqueous media which is significantly enhanced in presence of optimized concentration of SDS due to accumulation of the protonated drug molecules and electrostatically interaction with negatively charged micellar structures. As a result, the spherical micellar orientation of SDS onto the graphitic surface of SPEs offered the analytically sensitive determination of the target drugs over a wide linear concentration range with nano-molar detection limits possible negating the need for any complicated surface modifications. Finally, the proposed voltammetric method was successfully utilized in the individual determination of the target antihypertensive drugs in pharmaceutical formulations and human urine samples.

Oasis Malaria, Northern Mauritania1.

A malaria survey was conducted in Atar, the northernmost oasis city in Mauritania, during 2015-2016. All febrile patients in whom malaria was suspected were screened for malaria by using rapid diagnostic testing and microscopic examination of blood smears and later confirmed by PCR. Of 453 suspected malaria cases, 108 (23.8%) were positive by rapid diagnostic testing, 154 (34.0%) by microscopic examination, and 162 (35.7%) by PCR. Malaria cases were observed throughout the year and among all age groups. Plasmodium vivax was present in 120/162 (74.1%) cases, P. falciparum in 4/162 (2.4%), and mixed P. falciparum-P. vivax in 38/162 (23.4%). Malaria is endemic in northern Mauritania and could be spreading farther north in the Sahara, possibly because of human-driven environmental changes. Further entomologic and parasitologic studies and monitoring are needed to relate these findings to major Anopheles mosquito vectors and to design and implement strategies for malaria prevention and control.