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This study explored the alteration of naturally occurring radioactive materials (NORMs: 226Ra (≈238U), 232Th, 40K) in an anthropogenically disrupted urban river-basin (Turag, Bangladesh) in terms of constitutional substances (Sc, Ti, V, Fe, La, Ce, Sm, Eu, Tb, Dy, Ho, Yb, Lu, Hf, Ta, W, Th, U) of heavy-minerals. Average activity concentrations of 226Ra (≈238U), 232Th, and 40K were 41.5 ± 12.9, 72.1 ± 27.1, and 639 ± 100 Bqkg-1, respectively which were relatively higher compared to crustal origin. ∑REEs, Ta, W, Th, and U were ~2 times higher compared to crustal values with Ce and Eu-anomalies. APCS-MLR and PMF receptor models were used to determine the various anthropogenic and/or geogenic sources of NORMs and elements. Layer-wise variations of NORMs and elements were observed to trace the response of sedimentary processes towards the incoming pollution load. Presence of REEs indicates moderate degree of ecological risk to aquatic biota. However, carcinogenic risk (3.84 × 10-4 Sv-1) were significantly higher than threshold limit.
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Monitoramento de Radiação , Rios , Rios/química , Medição de Risco , Bangladesh , Poluentes Radioativos da Água/análise , Tório/análise , Minerais/análise , Radioisótopos de Potássio/análise , Rádio (Elemento)RESUMO
To investigate the interplay between varying anthropogenic activities and sediment dynamics in an urban river (Turag, Bangladesh), this study involved 37-sediment samples from 11 different sections of the river. Neutron activation analysis and atomic absorption spectrometry were utilized to quantify the concentrations of 14 metal(oid)s (Al, Ti, Co, Fe, As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Zn). This study revealed significant toxic metal trends, with Principal coordinate analysis explaining 62.91 % of the variance from upstream to downstream. The largest RSDs for Zn(287 %), Mn(120 %), and Cd(323 %) implies an irregular regional distribution throughout the river. The UNMIX-model and PMF-model were utilized to identify potential sources of metal(oid)s in sediments. â¼63.65-66.7 % of metal(oid)s in sediments originated from anthropogenic sources, while remaining attributed to natural sources in both models. Strikingly, all measured metal(oid)s' concentrations surpassed the threshold effect level, with Zn and Ni exceeding probable effect levels when compared to SQGs.
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Monitoramento Ambiental , Sedimentos Geológicos , Rios , Poluentes Químicos da Água , Sedimentos Geológicos/química , Rios/química , Poluentes Químicos da Água/análise , Bangladesh , Metais/análise , Metais Pesados/análiseRESUMO
This study examines the manufacturing, characterization, and biological evaluation of platinum nanoparticles, which were synthesized by Enterobacter cloacae and coated with Bovine Serum Albumin (BSA) and Resveratrol (RSV). The formation of PtNPs was confirmed with the change of color from dark yellow to black, which was due to the bioreduction of platinum chloride by E. cloacae. BSA and RSV functionalization enhanced these nanoparticles' biocompatibility and therapeutic potential. TGA, SEM, XRD, and FTIR were employed for characterization, where PtNPs and drug conjugation-related functional groups were studied by FTIR. XRD confirmed the crystalline nature of PtNPs and Pt-BSA-RSV NPs, while TGA and SEM showed thermal stability and post-drug coating morphological changes. Designed composite was also found to be biocompatible in nature in hemolytic testing, indicating their potential in Biomedical applications. After confirmation of PtNPs based nanocaompsite synthesis, they were examined for anti-bacterial, anti-oxidant, anti-inflammatory, and anti-cancer properties. Pt-BSA-RSV NPs showed higher concentration-dependent DPPH scavenging activity, which measured antioxidant capability. Enzyme inhibition tests demonstrated considerable anti-inflammatory activity against COX-2 and 15-LOX enzymes. In in vitro anticancer studies, Pt-BSA-RSV NPs effectively killed human ovarian cancer cells. This phenomenon was demonstrated to be facilitated by the acidic environment of cancer, as the drug release assay confirmed the release of RSV from the NP formulation in the acidic environment. Finally, Molecular docking also demonstrated that RSV has strong potential as an anti-oxidant, antibacterial, anti-inflammatory, and anticancer agent. Overall, in silico and in vitro investigations in the current study showed good medicinal applications for designed nanocomposites, however, further in-vivo experiments must be conducted to validate our findings.
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Nanopartículas Metálicas , Nanopartículas , Humanos , Soroalbumina Bovina/química , Nanopartículas Metálicas/química , Resveratrol/farmacologia , Platina/farmacologia , Platina/química , Antioxidantes/farmacologia , Simulação de Acoplamento Molecular , Nanopartículas/química , Anti-InflamatóriosRESUMO
To study the geogenic processes of naturally occurring radioactive materials' (NORMs') distribution, a transboundary Himalayan river (Punarbhaba) is chosen due to its trivial anthropogenic impacts. In explaining the genesis of radionuclides, transition elements (Sc, Ti, V, and Fe), rare-earth-elements (REEs: La, Eu, Ce, Yb, Sm, and Lu), Ta, Hf, Th, and U were analysed in 30 riverbed sediments collected from the Bangladeshi portion of the river. Elemental abundances and NORMs' activity were measured by neutron activation analysis and HPGe-gamma-spectrometry, respectively. Averagen=30 radioactivity concentrations of 226Ra (68.4 Bq kg-1), 232Th (85.7 Bq kg-1), and 40K (918 Bq kg-1) were 2.0-2.3-fold higher, which show elevated results compared to the corresponding world mean values. Additionally, mean-REE abundances were 1.02-1.38-times higher than those of crustal origin. Elevated (relative to earth-crust) ratios of Th/U (=3.95 ± 1.84) and 232Th/40K and statistical demonstrations invoke Th-dominant heavy minerals, indicating the role of kaolinite clay mineral abundance/granitic presence. However, Th/Yb, La/V, Hf/Sc, and Th/Sc ratios reveal the presence of felsic abundances, hydrodynamic sorting, and recycling of sedimentary minerals. Geo-environmental indices demonstrated the enrichment of chemical elements in heavy minerals, whereas radiological indices presented ionizing radiation concerns, e.g., the average absorbed-gamma-dose rate (123.1 nGy h-1) was 2.24-fold higher compared to the threshold value which might cause chronic health impacts depending on the degree of exposure. The mean excess lifetime cancer risk value for carcinogen exposure was 5.29 × 10-4 S v-1, which is â¼2-times greater than the suggested threshold. Therefore, plausible extraction of heavy minerals and using residues as building materials can alleviate the two-reconciling problems: (1) radiological risk management and (2) fluvial navigability.
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Metais Terras Raras , Monitoramento de Radiação , Radioatividade , Poluentes Radioativos do Solo , Metais Terras Raras/análise , Radioisótopos/análise , Minerais/análise , Medição de Risco , Poluentes Radioativos do Solo/análiseRESUMO
The "WaterHyacinth" dataset, a recently gathered collection of images featuring four distinct species of Water hyacinth from different regions of Bangladesh, is presented in this article. There are four different classifications: Lemna minor, Eichhornia crassipes, Monochoria korsakowii, and Pistia stratiotes. The collection consists of 1790 original images and in addition 4050 augmented photos of Water hyacinth species. Every original picture was captured with the appropriate background and in sufficient natural light. Every image was correctly placed in its corresponding subfolder, providing optimal use of the pictures by various machine learning and deep learning models. Researchers could make major progress in agriculture, environmental monitoring, aquatic science, and remote sensing domains by utilizing this enormous dataset and various machine learning and deep learning approaches. In addition to opening opportunities for significant developments in these domains, it offers an essential asset for further study.
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Groundwater is an essential resource in the Sundarban regions of India and Bangladesh, but its quality is deteriorating due to anthropogenic impacts. However, the integrated factors affecting groundwater chemistry, source distribution, and health risk are poorly understood along the Indo-Bangla coastal border. The goal of this study is to assess groundwater chemistry, associated driving factors, source contributions, and potential non-carcinogenic health risks (PN-CHR) using unsupervised machine learning models such as a self-organizing map (SOM), positive matrix factorization (PMF), ion ratios, and Monte Carlo simulation. For the Sundarban part of Bangladesh, the SOM clustering approach yielded six clusters, while it yielded five for the Indian Sundarbans. The SOM results showed high correlations among Ca2+, Mg2+, and K+, indicating a common origin. In the Bangladesh Sundarbans, mixed water predominated in all clusters except for cluster 3, whereas in the Indian Sundarbans, Cl--Na+ and mixed water dominated in clusters 1 and 2, and both water types dominated the remaining clusters. Coupling of SOM, PMF, and ionic ratios identified rock weathering as a driving factor for groundwater chemistry. Clusters 1 and 3 were found to be influenced by mineral dissolution and geogenic inputs (overall contribution of 47.7%), while agricultural and industrial effluents dominated clusters 4 and 5 (contribution of 52.7%) in the Bangladesh Sundarbans. Industrial effluents and agricultural activities were associated with clusters 3, 4, and 5 (contributions of 29.5% and 25.4%, respectively) and geogenic sources (contributions of 23 and 22.1% in clusters 1 and 2) in Indian Sundarbans. The probabilistic health risk assessment showed that NO3- poses a higher PN-CHR risk to human health than F- and As, and that potential risk to children is more evident in the Bangladesh Sundarban area than in the Indian Sundarbans. Local authorities must take urgent action to control NO3- emissions in the Indo-Bangla Sundarbans region.
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Água Subterrânea , Poluentes Químicos da Água , Criança , Humanos , Monitoramento Ambiental/métodos , Aprendizado de Máquina não Supervisionado , Agricultura , Água , Poluentes Químicos da Água/análise , Qualidade da ÁguaRESUMO
Naturally occurring radioactive materials (NORMs: 232Th, 226Ra, 40K) can reach our respiratory system by breathing of road dust which can cause severe health risks. Targeting the pioneering consideration of health risks from the NORMs in road dust, this work reveals the radioactivity abundances of NORMs in road dust from a megacity (Dhaka) of a developing country (Bangladesh). Bulk chemical compositions of U, Th, and K obtained from neutron activation analysis were converted to the equivalent radioactivities. Radioactivity concentrations of 226Ra, 232Th, and 40K in road dust ranged from 60-106, 110-159, and 488-709 Bq kg-1 with an average of 84.4 ± 13.1, 126 ± 11, and 549 ± 48 Bq kg-1, respectively. Estimated 226Ra, 232Th, and 40K radioactivities were, respectively, 1.7-3.0-, 3.7-5.3-, and 1.2-1.8-folds greater than the affiliated world average values. Mechanistic pathway of NORMs' enrichment and fractionation relative to the major origin (pedosphere) were evaluated concerning the water logging, relative solubility-controlled leaching and translocation, climatic conditions, and aerodynamic fractionations (dry and wet atmospheric depositions). Computation of customary radiological risk indices invokes health risks. Noticing the ingress of NOMR-holding dust into the human respiratory system along with the associated ionizing radiations, the computed radiological indices represent only the least probable health hazards. Nevertheless, in real situations, α-particles from the radioactive decay products of 232Th and 238U can create acute radiation damages of respiratory system. Policymakers should emphasize on limiting the dust particle evolution, and public awareness is required to alleviate the health risks.
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Monitoramento de Radiação , Radioatividade , Poluentes Radioativos do Solo , Humanos , Poeira/análise , Bangladesh , Poluentes Radioativos do Solo/análise , Tório/análiseRESUMO
Tannery-effluent is one of the top-ranked hazardous waste which is generally discharged into the river. To study the fluvial response toward the tannery-effluents and to trace anthropogenic foot-prints in the fluvial-system, a suite of systematically collected sediment and water samples were analyzed for radioactive (226Ra, 232Th, and 40K) and non-radioactive elements (Al, Ti, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Sb, Hg, and Pb). Neutron activation analysis and atomic absorption spectroscopy were used for elemental analysis, whereas HPGe-gamma-detector was used for measuring the primordial-radionuclides. Ranges of Cr-abundances in sediment and water were 63-4373 µg.g-1 and 15.6-52.2 µg.L-1, respectively which were â¼4-14 times higher than the geological background. Radioactivity concentrations of 226Ra, 232Th, and 40K ranged from 17.7-48.5, 36.1-81.6, and 687-1041 Bq.kg-1, respectively which were significantly depleted in effluent discharge point. Hence, primordial-radionuclides were used as natural tracers for tracing anthropogenic foot-prints which were explained in terms of dilution effect, redox environment and differential geo-environmental events/characteristics. From statistical-approaches and geochemical reasoning, elemental sources and responses in fluvial system were explored. Surprisingly, ecological & radiological risks were reduced while sediment quality guideline-based ecotoxicity & water-mediated health risks were increased by the incorporation of tannery effluents. This study describes the sedimentary response toward the received tannery effluents which is particularly explored by the primordial radionuclides.
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Metais Pesados , Poluentes Químicos da Água , Monitoramento Ambiental/métodos , Rios/química , Poluentes Químicos da Água/análise , Água/análise , Radioisótopos/análise , Metais Pesados/análise , Sedimentos Geológicos/químicaRESUMO
This study was carried out on a negligible anthropogenically impacted Indo-Bangla transboundary river basin (Atrai, Bangladesh) to elicit radionuclides' and elemental distributions. Thirty sediment samples were collected from the Bangladesh portion of the river, and instrumental neutron activation analysis and HPGe γ-Spectrometry techniques were used to determine environmental radionuclides (e.g., 232Th, 226Ra, 40K) and associated elemental concentrations, respectively. Metal concentrations (Sc, V, Fe, Eu, Sm, La, Yb, Ce, Lu, Ta, Hf) were determined to comprehend the genesis of greater radioactivity. Recognizing the mean concentration of absorbed gamma dose rate (158.7 hGyh-1) is 2.88-times more than the recommended value (55 hGyh-1) that describes ionizing radiation concerns regarding potential health risks to the surrounding communities and the houses of native residents, which are constructed by Atrai river sediment. This work will assist relevant policymakers in exploring valuable heavy minerals and provide information regarding radiological health risks from a fluvial system.
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Exposição à Radiação , Radioatividade , Metais/análise , Rios/química , Radioisótopos/análise , Exposição à Radiação/análise , Monitoramento AmbientalRESUMO
Aspergillosis is a rising concern worldwide; however, its prevalence is not well documented in China. This retrospective study determined Aspergillus's epidemiology and antifungal susceptibilities at Meizhou People's Hospital, South China. From 2017 to 2022, the demographic, clinical, and laboratory data about aspergillosis were collected from the hospital's records and analysed using descriptive statistics, chi-square test, and ANOVA. Of 474 aspergillosis cases, A. fumigatus (75.32%) was the most common, followed by A. niger (9.92%), A. flavus (8.86%), and A. terreus (5.91%). A 5.94-fold increase in aspergillosis occurred during the study duration, with the highest cases reported from the intensive care unit (52.74%) - chronic pulmonary aspergillosis (79.1%) and isolated from sputum (62.93%). Only 38 (8.02%) patients used immunosuppressant drugs, while gastroenteritis (5.7%), haematologic malignancy (4.22%), and cardiovascular disease (4.22%) were the most prevalent underlying illnesses. In A. fumigatus, the wild-type (WT) isolates against amphotericin B (99.1%) were higher than triazoles (97-98%), whereas, in non-fumigatus Aspergillus species, the triazole (95-100%) WT proportion was greater than amphotericin B (91-95%). Additionally, there were significantly fewer WT A. fumigatus isolates for itraconazole and posaconazole in outpatients than inpatients. These findings may aid in better understanding and management of aspergillosis in the region.
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Antifúngicos , Aspergilose , Humanos , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Anfotericina B , Estudos Retrospectivos , Voriconazol , Aspergillus , Aspergilose/tratamento farmacológico , Aspergilose/epidemiologia , Aspergilose/microbiologia , Testes de Sensibilidade MicrobianaRESUMO
Tobacco products are widely recognized as a major contributor to death. Cigarette smoke contains several toxic chemicals including heavy metals particulate causing high health risks. However, limited information has been available on the health risks associated with the heavy metals in cigarettes commonly sold in the Bangladeshi market. This study evaluated the concentrations and potential health risks posed by ten concerned heavy metals in ten widely consumed cigarette brands in Bangladesh using an atomic absorption spectrometer. The concentration (mg/kg) ranges of heavy metals Pb, Cd, Cr, As, Co, Ni, Mn, Fe, Cu, and Zn vary between 0.46-1.05, 0.55-1.03, 0.80-1.2, 0.22-0.40, 0.46-0.78, 2.59-3.03, 436.8-762.7, 115.8-184.4, 146.6-217.7, and 34.0-42.7, respectively. We assume that the heavy metals content among cigarette brands is varied due to the differences in the source of tobacco they use for cigarette preparation. The carcinogenic risks posed by heavy metals follow the order of Cr > Co > Cd > As > Ni > Pb, while the non-carcinogenic risks for Cu, Zn, Fe, and Mn were greater than unity (HQ > 1), except for Fe. The existence of toxic heavy metals in cigarette tobacco may thus introduce noticeable non-carcinogenic and carcinogenic health impacts accompanying inhalation exposure. This study provides the first comprehensive report so far on heavy metal concentration and associated health risks in branded cigarettes commonly sold in Bangladesh. Hence, this data and the information provided can serve as a baseline as well as a reference for future research and have potential implications for policy and legislation in Bangladesh.
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The study aimed to synthesize zinc nanoparticles (Zn-NPs) using an aqueous extract derived from Acacia modesta Wall. leaves. Several characterization techniques were employed to confirm the successful formation of zinc nanoparticles. UV-visible spectrophotometry indicated a peak at 374 nm, validating the bioreduction process. Scanning electron microscopy (SEM) was utilized to analyze the morphology, transmission electron microscopy (TEM) to determine particle size and shape, X-ray diffraction (XRD) for crystalline structure analysis, energy-dispersive X-ray spectroscopy (EDX) for elemental composition and Fourier-transform infrared spectroscopy (FTIR) to identify functional groups. The synthesized Zn-NPs demonstrated remarkable antibacterial activity against Escherichia coli (95% inhibition) and moderate antifungal activity against Candida albicans (70% inhibition). In phytotoxicity tests, the Zn-NPs exhibited a 55% reduction in the growth of Lamina minor at the highest dilution (1000 µl). Based on these findings, the study concluded that the green-synthesized Zn-NPs hold great potential as effective antibiotics against pathogenic bacteria and could be utilized in various industrial and agricultural applications.
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Acacia , Fabaceae , Nanopartículas Metálicas , Zinco , Agricultura , Antibacterianos/farmacologia , Escherichia coliRESUMO
This study utilized surface sediments from a potentially less polluted transboundary Himalayan River (Brahmaputra: China-India-Bangladesh) to investigate the abundance of 15 geochemically and ecologically significant elements and to predict their sources and ecological consequences. INAA was applied to determine the elemental concentrations. The average abundances (µg.g-1) of Rb (94.20), Cs (4.49), Th (20.31), & U (2.73) were 1.12-2.26 folds elevated than shale. Environmental indices disclosed a pollution status ranging from "uncontaminated to moderately contaminated," with minimal Rb, U, and Th enrichment in the downstream zone. Consensus-based sediment quality guideline (SQG) threshold values suggested that only Cr (60% samples > TEL) may impose rare biological effects. Ecological risk indices suggested "minor to no" possible eco-toxicological risks for the accounted elements (Cr, Co, Mn, Zn, Sb, & As). The positive matrix factorization (PMF) model predicated the predominance of geogenic or crustal contributions (â¼72.69%) for Al, K, Na, Ti, Co, Zn, Ba, Cs, As, Rb, Th, & U derived from elemental fractionations, mineral weathering, and bio-geo-chemical mobilization. The relative contributions of anthropogenic sources (â¼27.31%; such as the construction of roads, settlement expansion, litter disposal, municipal waste discharge, mining activities, agricultural encroachment, etc.) on elemental distribution were significantly lower. The abundance of Cr and Mn was mainly influenced by anthropogenic sources. This study demonstrated the effectiveness of utilizing geo-environmental guidelines and receptor models in discriminating the natural & anthropogenic origins of metals in the complex riverine sediments of a less anthropogenically affected river.
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Metais Pesados , Poluentes Químicos da Água , Metais Pesados/análise , Monitoramento Ambiental , Sedimentos Geológicos , Poluição Ambiental/análise , Medição de Risco , China , Poluentes Químicos da Água/análiseRESUMO
Toxic metal(loid)s (TMLs) in agricultural soils cause detrimental effects on ecosystem and human health. Therefore, source-specific health risk apportionment is very crucial for the prevention and control of TMLs in agricultural soils. In this study, 149 surface soil samples were taken from a coal mining region in northwest Bangladesh and analyzed for 12 TMLs (Pb, Cd, Ni, Cr, Mn, Fe, Co, Zn, Cu, As, Se, and Hg). Positive matrix factorization (PMF) and absolute principal component score-multiple linear regression (APCS-MLR) receptor models were employed to quantify the pollution sources of soil TMLs. Both models identified five possible sources of pollution: agrochemical practice, industrial emissions, coal-power-plant, geogenic source, and atmospheric deposition, while the contribution rates of each source were calculated as 28.2%, 17.2%, 19.3%, 19% and 16.3% in APCS-MLR, 22.2%, 13.4%, 24.3%, 15.1% and 25.1% in PMF, respectively. Agrochemical practice was the major source of non-carcinogenic risk (NCR) (adults: 32.37%, children: 31.54%), while atmospheric deposition was the highest source of carcinogenic risk (CR) (adults: 48.83%, children: 50.11%). NCR and CR values for adults were slightly higher than for children. However, the trends in NCR and CR between children and adults were similar. As a result, among the sources of pollution, agrochemical practices and atmospheric deposition have been identified as the primary sources of soil TMLs, so prevention and control strategies should be applied primarily for these pollution sources in order to protect human health.
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Metais Pesados , Poluentes do Solo , Adulto , Criança , Humanos , Solo , Metais Pesados/toxicidade , Metais Pesados/análise , Bangladesh , Ecossistema , Monitoramento Ambiental , Poluentes do Solo/toxicidade , Poluentes do Solo/análise , Carcinógenos , Agroquímicos , China , Medição de RiscoRESUMO
Pentavalent antimonials continue to be the standard treatment for cutaneous leishmaniasis. But their use is retarded owing to highly-priced, prolonged hospitalization, noxious and poor solubility. Therefore, there is a dire need to characterize new potential compounds possessing anti-leishmanial activity. Topical therapies that are more successful are an essential alternative therapeutic option for the localized self-limiting form of this disease. We tested the herbal-based topical cream Lesh Nat B against Leishmania tropica KWH23 promastigotes and axenic amastigotes in vitro. The anti-leishmanial activity of Lesh Nat B cream was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against promastigotes and axenic amastigotes. The results of Lesh Nat B cream were concentration and incubation time-dependent. After 72 h of incubation, Lesh Nat B cream efficiently suppresses the promastigote form of the parasite, followed by 48 h and 24 h. At 72 h, the lowest and highest levels of activity were 37% and 90%. Amastigotes had a minimum activity of 34% and a maximum activity of 78.5%, respectively. This formulation was more cytotoxic against promastigote form than amastigotes form at 72 h incubation periods. All the experiments were carried out in triplicates. Half-maximal inhibitory concentration (IC50) values were determined to be (66 ug/ml) and (70 ug/ml) against promastigote and amastigote forms, respectively. Moreover, 1.63% hemolytic activity was observed in Lesh Nat B cream at (10 µg/ml) while 3% hemolytic activity was observed at (37 µg/ml). It can be concluded that Lesh Nat B cream demonstrated effective Leishmanicidal and less hemolytic activity and can be used as an alternative therapeutic option for the treatment of cutaneous leishmaniasis; however, more studies are expected to justify its effectiveness in treating cutaneous leishmaniasis in both humans and animals.
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Due to limited chemotherapeutic options for leishmaniasis, novel synthetic compounds are gaining attention for evaluation against leishmaniasis. This study aimed to synthesize the compound's Schiff bases of Vanillin to investigate and evaluate their anti-leishmanial potentials against intracellular protozoan parasites Leishmania tropica. In the current study, the phenomena of synergism by designing Schiff bases with Vanillin enhances their desired importance. A total of five compounds Schiff bases of Vanillin were synthesized using different aromatic amines and Vanillin. The structural analysis of all the compounds was done through FT-IR (Fourier Transformer-Infrared), thin layer chromatography, and spectroscopic techniques such as 13C-NMR, mass spectrometry, and 1H-NMR. The antimicrobial properties of all the compounds ZI-1, ZI-2, BS-1, KH-1, and FA-2 against promastigotes and amastigotes forms of L. tropica were analyzed at three different concentrations 25, 50, and 100 µg/ml. The in-vitro MTT assay was performed to calculate the percent inhibition, IC50 values, and their cytotoxicity. The highest percent inhibition values against promastigote form of L. tropica were BS-1 53.78% at 25 µg/ml, ZI-2 66.95% at 50 µg/ml, and again ZI-2 76.92% at 100 µg/ml. Similarly, the highest percent inhibition values against intracellular amastigote stage were BS-1 55.77% at 25 µg/ml, ZI-2 67.78% at 50 µg/ml and again ZI-2 84.93% 100 µg/ml. The highest potency was recorded for BS-1 in both stages, with IC50 values of 9.83 and 4.27 µg/ml against promastigotes and intracellular amastigotes, respectively. The percent hemolysis as toxicity; the lowest percent hemolysis was recorded for ZI-1 at three different concentrations of 25, 50, 100 µg/ml of 2.60, 3.50, and 6.31, respectively. These results suggested that all the compounds exhibited anti-leishmanial activity, with BS-1 as the most potent. Further studies are suggested to increase the activity of compounds with structural modifications by the addition of some other synergistic, novel, and analogue compounds.
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The coastal areas of Bangladesh have poor accessibility to fresh drinking water and the groundwater is not suitable for drinking, cooking, and other domestic uses due to high levels of salinity and potentially toxic elements. The current study focuses on understanding of the distribution of some physicochemical parameters (temperature, pH, EC, TDS, and salinity) and chemical elements (Fe, Mn, Zn, Ca, Mg, Na, K, Cu, Co, Pb, As, Cr, Cd, and Ni) with health perspective in drinking water from the southwestern coastal area of Bangladesh. The physicochemical properties of the water samples were examined with a multiparameter meter, while the elemental concentrations were analyzed using atomic absorption spectrometer. Water quality index (WQI) and irrigation indices were utilized to determine the drinking water quality and irrigation feasibility, respectively, whereas hazard quotients (HQs) and hazard index (HI) were used to assess the probable pathways and the associated potential risks to human health. The concentrations of some toxic elements in measured samples were relatively higher compared to drinking water guidelines, indicating that ground and surface water are not apt for drinking and/or domestic uses. The multivariate statistical approaches linked the source of the pollutants in the studied water body mostly to the geogenic origin including saline water intrusion. WQI values ranged from 18 to 430, reflecting excellent to unsuitable categories of water quality. The assessment of human health risks due to exposure to contaminated water demonstrated both carcinogenic and non-carcinogenic health risks in the exposed residents of the study area. Therefore, appropriate long-term coastal area management strategies should be adopted in the study region for environmental sustainability. The findings of this research will be supportive in understanding the actual situation of fresh drinking water in the area for policymakers, planners, and environmentalists to take effective necessary measures to ensure safe drinking water in the study area.
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Água Potável , Água Subterrânea , Metais Pesados , Poluentes Químicos da Água , Humanos , Qualidade da Água , Monitoramento Ambiental , Bangladesh , Poluição da Água , Água Subterrânea/química , Poluentes Químicos da Água/análise , Medição de Risco , Metais Pesados/análiseRESUMO
Rising soil pollution has recently emerged as a significant global issue as a result of increased industrialization, urbanization, and inadequate waste management. In Rampal Upazila, soil contamination with heavy metals resulted in a significant deterioration of quality of life and life expectancy, so the study's goal is to appraise the level of heavy metal contamination in soil samples. Inductively coupled plasma-optical emission spectrometry was used to identify 13 heavy metals (Al, Na, Cr, Co, Cu, Fe, Mg, Mn, Ni, Pb, Ca, Zn, and K) from 17 soil samples that were collected at random from Rampal. Enrichment factor (EF), geo-accumulation index (Igeo), contamination factor (CF), pollution load index, elemental fractionation, and potential ecological risk analysis were used to evaluate the level of pollution and sources of metal. The average concentration of heavy metals implies that they are below in the permissible limit except for Pb. Environmental indices also showed the same result for Pb. The ecological risk index (RI) for six elements-Mn, Zn, Cr, Fe, Cu, and Pb-is 26.575. For investigating the behavior and origin of elements, multivariate statistical analysis was also applied. From the EF, Na, Cr, Fe, and Mg are in the anthropogenic region, and Al, Co, Cu, Mn, Ni, Ca, K, and Zn are minorly polluted, but Pb is highly contaminated in the Rampal area. The geo-accumulation index exhibits that Pb is slightly contaminated but others are not, while CF shows no contamination in this region. From the ecological RI, the value which is below 150 is called uncontaminated, which indicates that our studied area is ecologically free. There are various classifications of heavy metal contamination in the study area. Therefore, regular monitoring of soil pollution is required, and the public awareness needs to be raised to ensure a safe environment.