RESUMO
The comparative study of the immunogenic and protective properties of V. cholerae, grown in vivo and in vitro, and cell walls and lysates obtained from these organisms. In the mouse protection test the efficacy of preparations obtained from vibrios grown in vivo did not exceed that of the preparations obtained from V. cholerae agar cultures.
Assuntos
Vibrio cholerae/imunologia , Animais , Vacinas Bacterianas/imunologia , Vacinas Bacterianas/toxicidade , Membrana Celular/imunologia , Cólera/prevenção & controle , Meios de Cultura , Relação Dose-Resposta Imunológica , Imunização/métodos , Dose Letal Mediana , Camundongos , Coelhos , Vacinas de Produtos Inativados/imunologia , Vacinas de Produtos Inativados/toxicidade , Vibrio cholerae/crescimento & desenvolvimento , Vibrio cholerae/patogenicidade , Virulência/imunologiaRESUMO
A high degree of resistance to cholera diagnostic phages and carriership of prophages characteristic of V. cholerae eltor strains vct+ were shown to be the specific features V. cholerae isolated in Daghestan during the period of June-October 1994. Among the strains under study, isolated respectively in 12 and 18 out of 19 regions of Daghestan, a high proportion was found to have resistance to tetracycline (65%) and chloramphenicol (28.6%). Moreover, some strains were found to be resistant to furagin and erythromycin. Out of 242 strains resistant to antibacterial preparations, 163 strains were found to have multiple resistance. Gentamicin, cipropfloxacin and doxicycline were shown to have high in vitro activity with respect to the strains under study.
Assuntos
Vibrio cholerae/isolamento & purificação , Antibacterianos/farmacologia , Tipagem de Bacteriófagos , Portador Sadio/microbiologia , Cólera/microbiologia , Daguestão , Resistência Microbiana a Medicamentos , Microbiologia Ambiental , Humanos , Testes de Sensibilidade Microbiana , Vibrio cholerae/classificação , Vibrio cholerae/efeitos dos fármacosRESUMO
V. eltor strain 1310, serotype Ogawa, an original strain capable of producing cholera enterotoxin (exotoxin) in synthetic culture media, has been obtained. The exotoxin produced by this strain can be obtained in large amounts and used as cholerigenic toxoid for immunization against cholera.