High frequency of BRAF V600E mutation in Iranian population ameloblastomas.

BACKGROUND: Ameloblastoma is a common locally invasive but slow-growing neoplasm of the jaws with an odontogenic origin. Association between BRAF V600E mutation and clinicopathologic features and behavior of ameloblastoma remains controversial. This study aimed to evaluate BRAF V600E gene mutation and expression of its related proteins with clinicopathologic parameters in conventional ameloblastoma. MATERIAL AND METHODS: 50 Formalin-fixed paraffin-embedded blocks were included in this study. Immunohistochemistry was done using rabbit monoclonal BRAF V600E mutation-specific antibody VE1. Quantitative real-time polymerase chain reaction assay was used for evaluating of BRAF V600E mutation. RESULTS: Expression of BRAF V600E antibody was Positive in 42 out of 50 cases (84%). 46 (92%) out of 50 specimens showed BRAF V600E mutation. There were 13 cases of recurrence (26%). 3 out of 4 cases with negative mutations did not show recurrence. CONCLUSIONS: We report the highest frequency (92%) of BRAF V600E mutation in ameloblastomas in the Iranian population. Although there was not a significant association between BRAF V600E­positive immunoexpression and recurrence and clinicopathologic parameters, its high frequency could emphasize its role as a therapeutic marker in the future.

Assessment of Bond Strength between Metal Brackets and Non-Glazed Ceramic in Different Surface Treatment Methods.

OBJECTIVE: The aim of this study was to evaluate the bond strength between metal brackets and non-glazed ceramic with three different surface treatment methods. MATERIALS AND METHODS: Forty-two non-glazed ceramic disks were assigned into three groups. Group I and II specimens were etched with 9.5% hydrofluoric acid. Subsequently in group I, silane and adhesive were applied and in group II, bonding agent was used only. In group III, specimens were treated with 35% phosphoric acid and then silane and adhesive were applied. Brackets were bonded with light-cured composites. The specimens were stored in water in room temperature for 24 hours and then thermocycled 500 times between 5°C and 55°C. RESULTS: The difference of tensile bond strength between groups I and III was not significant (P=0.999). However, the tensile bond strength of group II was significantly lower than groups I, and III (P<0.001). The adhesive remnant index scores between the three groups had statistically significant differences (P<0.001). CONCLUSION: With the application of scotch bond multi-purpose plus adhesive, we can use phosphoric acid instead of hydrofluoric acid for bonding brackets to non-glazed ceramic restorations.

Radiographic Vertical Bone Loss Evaluation around Dental Implants Following One Year of Functional Loading.

OBJECTIVE: Vertical bone loss evaluations in the Nobel Biocare Replace(®) Select Tapered (™) implant system in the human after one-year loading time. MATERIALS AND METHODS: This retrospective cross-sectional study was performed on 31 patients (14 men, 17 women; mean age, 60.39 years) receiving 170 implants (mean, 5.48 for each patient) of Groovy and Non-groovy designs in the Nobel Biocare Replace(®) Select Tapered (™) system. The marginal bone loss was measured at mesial and distal aspects of the implants on OPG x-rays after one-year follow-up. The data regarding the patient's gender, age, history of disease, smoking, bone type at implant location, loading time of prosthesis and implant, implant design, diameter and length were recorded by the patients' records and interview. The data were subjected to multiple linear regression and Pearson coefficient ratio regarding different factors. RESULTS: The mean (standard deviation) distal, mesial and overall bone loss was 0.688 mm (0.851), 0.665 mm (0.849) and 0.935 mm (0.905), respectively in the studied implants. No significant differences were found regarding implant location, bone quality at the implant region, implant design and bone graft reception. In addition, no significant correlation was found between the occurred bone loss and implant diameter, length and number of used splints. CONCLUSION: Due to the criteria mentioned for implant success in term of bone loss values after one-year loading time, Noble Biocare Replace(®) Select Tapered (™) implant system is an acceptable treatment option for implant restorations in this regard.