Comparison of Fragments in Human Hemostatic Proteins That Mimics Fragments in Proteins of A/H1N1 Viruses and Coronaviruses.

Objective: To compare the repertoire of proteins of the human hemostatic system and fragments mimicking these proteins in the proteins of influenza A/H1N1 viruses and coronaviruses. Material and methods. Influenza viruses A/H1N1 (A/Brevig Mission/1/18), A/St. Petersburg /RII04/2016 (H1N1)pdm09, coronaviruses SARS-CoV and SARS-CoV-2 (strain Wuhan-Hu-1) were used for comparative computer analysis. The sources of the primary structures of proteins of the analyzed viruses and 41 proteins of the human hemostatic system were publicly available Internet databases, respectively, www.ncbi.nlm.nih.gov and www.nextprot.org. The search for homologous sequences in the structure of viral proteins and hemostatic proteins was carried out by comparing fragments of 12 amino acids in length, taking as related those that showed identity at ≥8 positions. Results. Comparative analysis of the repertoire of cellular proteins of the hemostatic system and fragments mimicking these proteins in the structure of proteins of viruses A/H1N1 1918, A(H1N1)pdm09 isolated in 2016, SARS-CoV and SARS-CoV-2, showed a significant difference between SARS-CoV-2 and analyzed viruses. In the protein structure of the SARS-CoV-2 virus, mimicry was revealed for almost all analyzed hemostasis proteins. As for the comparison of viruses A/H1N1 1918, A(H1N1)pdm09 2016 and SARS-CoV, the influenza virus A/H1N1 1918 and SARS-CoV are the closest in the repertoire of hemostatic proteins. Conclusion. Obtained bioinformatic analysis data can serve as a basis for further study of the role of homologous fragments in the regulation of hemostasis of the host organism.

[Brain plasticity: limitations and possibilities]. / Plastichnost' mozga: ogranicheniia i vozmozhnosti.

Biological limitations related to the brain regeneration and stem cells transplantation as well as the factors influencing the brain plasticity following the brain injury, including epigenetic regulatory mechanisms, are presented. Non-invasive transcranial microelectrostimulation is considered as a perspective method of polysystemic influence on endogenic mechanisms of brain recovery.

[Arterial hypertension: an expanding pathogenic continuum and therapeutic limitations].

The paper considers the role of the immune system, endoplasmic network stress, metabolic dysregulation, and epigenetic mechanisms in the pathogenesis of essential hypertension, as well as the limited possibilities of therapy with existing antihypertensive agents.

[Heart failure in cardiorenal syndromes].

This review considers the classification of cardiorenal syndromes (CRS), the pathogenesis of heart failure, and a search for therapeutic targets for its treatment, biomarkers for heart and kidney failure, difficulties in therapy and reversion of the pathogenetic continuum of CRS, and promises for the use of some new technologies to treat this condition.

[Heart failure: the pathogenetic continuum and biomarkers].

Molecular aspects of pathogenesis of cardiac failure touching on transmission of pathological signals through the transduction system, involvement of mechanisms of transcriptional reprogramming and metabolic changes in the myocardium, as well as processes of aging and inflammation in the pathogenetic continuum are considered in this paper. Data on markers of early diagnosis, prognosis and progression of heart failure and perspectives of discovery of its pathogenesis within framework of developing system biology integrating analysis of multiple different in their nature biomarkers are also presented.

[The influence of prolonged transthoracic microelectrostimulation on the course of myocardial infarction].

The subjects of the study were patients with acute myocardial infarction, who underwent prolonged transthoracic microelecrostimulation. The procedure had certain immediate effects, such as antinociceptive, anxiolytic, hypotensive, and somnific effect, lead to positive emotional shifts and substantial decrease of the degree of circulatory insufficiency, and to positive ECG dynamics. It also had long-time effects, such as positive myocardial remodeling, manifesting in the stabilization of end diastolic volume, lowering of end systolic volume and thus increase of ejection fraction, according to EchoCG results. The article substantiates the influence of transthoracic microelecrostimulation on the healing of infarcted myocardium.

[Treatment of infantile cerebral paralysis using transcraniovertebral microelectric stimulation]. / Transkraniovertebral'naia mikroélektrostimuliatsiia v terapii detskogo tserebral'nogo paralicha.

A new method of therapy of children with cerebral palsy (CP) has been elaborated. Transitory consequent action of weak bipolar low-frequency current (30-100 mcA, 2-10 Hz) on different levels of motor activity regulation (brain and spinal cord, effectory organs) resulted in a stable correction of both motor and other types of disorders in CP patients. Therapeutic course included 2-8 procedures, 1-2 weekly, their duration 3-5 minutes. Depending on the reaction on the transcraniovertebral microelectrostimulation (MES) the patients were divided into fast and slow reactive groups. Different reaction of the children to MES reflected, probably, a variety of pathogenetic CP mechanisms and a mosaic of cerebral damage. Corrective MES action on the patients' brain was polysystemic and was, probably, based on the changes in integral brain mechanisms, voluminous transmission and homeostasis of brain, in particular.

[The opiate-like and antimicrobial bioactivities of food protein peptides]. / Opiatopodobnaia i antimikrobnaia bioaktivnosti peptidov nekotorykh pishchevykh belkov.

At least four food peptides were found to possess opiate-like and antibacterial properties, lys-arg-fen-ala-glu being the most active one. These peptides seem to be able to become accumulated in high concentrations in indigestion and induce an intestinal spasm and constipation.