1.
J Microbiol Methods
; 148: 18-21, 2018 05.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29596960
RESUMO
Bacillus subtilis pheS was genetically modified to obtain a counter-selection marker with high selection efficiency in Bacillus amyloliquefaciens. The application of the new replication-thermosensitive integrative vector pNZTM1, containing this marker, pheSBsT255S/A309G, with a two-step replacement recombination procedure provides an effective tool for the genetic engineering of industrially important Bacillus species.