[Level of plasma fibronectin and its biological activity in patients with ovarian cancer]. / Uroven' plazmennogo fibronektina i ego biologicheskaia aktivnost' u bol'nykh rakom iaichnikov.

Plasma level of fibronectin and its biological activity were assessed in 33 females suffering ovarian cancer; 13 cases had stage I or II disease whereas 20-stage III or IV tumors. Fibronectin level, as measured by immunoelectrophoresis, proved normal. This was accompanied by a statistically significant (p less than 0.05) rise in gelatin--binding activity of the glycoprotein in patients with stage I-II cancer which accounted for an increased value registered for the entire group studied. However, no such rise was observed in cases of stage III and IV disease.

[Biological effects of Gelatinol during artificial circulation]. / Biologicheskie éffekty zhelatinolia pri iskusstvennom krovoobrashchenii.

The levels of plasma fibronectin (FN) were investigated in patients during heart surgery using artificial circulation. Plasma FN levels were assessed by electroimmunoassay (rocket immunoelectrophoresis) and bioassay (aggregation of gelatin-coated microparticles). The application of gelatinol in hemodilution++ composition led to the depletion of bioactive Fn from 140 to 0 micrograms/ml, whereas immunoreactive Fn levels were slightly elevated. The in vitro experiments have shown that gelatinol inhibits the aggregation of gelatinol-coated microparticles and chemiluminiscence of neutrophils, stimulated by gelatin-coated particles. Gelatinol elimination from hemodilution++ composition resulted in the reduction of postoperative mortality and accelerated tissue reparation.

[The effect of fibronectin preparations on the chemiluminescence of neutrophils]. / Vliianie preparatov fibronektina na khemiliuminestsentnyi otvet neitrofilov.

Effect of some fibronectin preparations on luminol-dependent chemiluminescence of neutrophils was studied. These fibronectin preparations were obtained after heat denaturation and tryptic digestion. Native fibronectin cause aggregation of gelatin-coated particles and dose-dependent promotion of chemiluminescence of neutrophils, while the rate of these reactions was distinctly decreased if the heat denaturated preparations of fibronectin were used. Structural integrity of fibronectin molecule appears to be responsible for promotion of the neutrophil chemiluminescence.

[Plasma fibronectin in complicated forms of respiratory viral infections in young children]. / Plazmennyi fibronektin pri oslozhnennykh formakh respiratornykh virusnykh infektsii u detei rannego vozrasta.

As many as 89 infants with acute respiratory viral infections (ARVI) were examined to establish a decrease of the levels of immunochemical and bioactive fibronectin, seen during the marked clinical manifestations of the disease. Changes in the level of fibronectin in the course of ARVI are reversible in nature, being related to the intensity of the general toxic manifestations. Measurements of bioactive fibronectin are most informative for assessing the degree of intoxication. Profound and long-term reduction of the level of bioactive fibronectin may be seen in grave focal confluent and polysegmental pneumonia.

[A method of determining the concentration of biologically active fibronectin]. / Metod opredeleniia kontsentratsii biologicheski aktivnogo fibronektina.

The suggested method is based on the ability of fibronectin intact molecules to agglutinate gelatin-coated particles. Gelatin-coated particles have been prepared with the use of specially treated Staphylococcus epidermidis cells. The concentration of biologically active fibronectin has been measured using laser nephelometry and agglutination test in microtitre plates. The accuracy of the test is 4 mg/ml (laser nephelometer) and 0.1 mg/ml (agglutination test).