[The approval of an immunoenzyme test system based on the F(ab)2 fragments of staphylococcal antibodies for determining staphylococcal alpha-hemolysin]. / Aprobatsiia immunofermentnoi test-sistemy na osnove F(ab)2-fragmentov protivostafilokokkovykh antitel dlia opredeleniia stafilokokkocogo al'fa-gemolizina.

In the approbation of the enzyme-linked immunosorbent assay (ELISA) system on the basis of F(ab)2 fragments of antistaphylococcal antibodies on 307 cultures of the representatives of the genera Staphylococcus, Pseudomonas and Proteus high sensitivity, specificity and effectiveness of ELISA for the quantitative and qualitative evaluation of the alpha-hemolytic activity of S.aureus were established. The ELISA system has made it possible to additionally detect alpha-hemolysin in 62% of S.aureus strains classified with as nontoxigenic strains using hemolysis test in Petri dishes. The sensitivity limit of this method is 0.0005 binding units or 1.0 ng in terms of protein content. The use of the ELISA system may be recommended for the study of the toxigenic properties of staphylococci.

[Detection of diphtheria toxin and its degradation products by using immunoenzyme analysis]. / Vyiavlenie difteriinogo toksina i produktov ego degradatsii s pomoshch'iu immunofermentnogo analiza.

An enzyme immunoassay system for the detection of diphtheria toxin and the products of its degradation has been developed on the basis of the enzyme-linked immunosorbent assay. To sensitize the assay plates, bivalent F(ab)2-fragments of purified antidiphtheria antibodies at a concentration of 10.0 micrograms/ml, obtained from the blood serum of hyperimmunized rabbits, have been used. Specific conjugates have been prepared with the use of F(ab)2-fragments of purified antidiphtheria antibodies obtained from the blood serum of hyperimmunized horses. The optimum time and temperature conditions of the assay have been established. The new enzyme immunoassay system permits the detection of diphtheria toxin and the products of its degradation in biological substrates at a concentration of 10.0-5.0 ng/ml.

[Toxicity of Bordetella pertussis suspensions associated with the preparation of pertussis vaccine]. / Izuchenie toksichnosti suspenzii Bordetella pertussis v sviazi s izgotovleniem kokliushnoi vaktsiny.

The process of the detoxication of B. pertussis suspensions during their storage has a wave-like character and is determined by changes in the levels of the toxicity of the soluble and corpuscular fractions. Conditions facilitating the transition of toxic cellular products into the soluble state may lead to the increase of the toxic activity of pertussis vaccines.