Potential Hepatoprotective Effect of Matricaria Pubescens on High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Rats.

This study aimed to identify the phytochemical compounds of Matricaria pubescens by LC-MS/MS and evaluate the potential protective effect of its supplementation in high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in adult rats through modulation of oxidative stress and histopathological changes. Twenty-four male rats were randomly divided into four groups. The first group served as control and received the standard diet. The second group (HFD) received a high-fat diet only (30 % of sheep fat). The third group's (control+MP) animals received a standard diet supplemented with 5 % M. pubescens (w/w). The fourth group (HFD+MP) received a high-fat diet supplemented with 5 % M. pubescens for 16 weeks. LC-MS/MS analysis showed that M. pubescens contains many phytochemical compounds. It was observed that the ethanolic extract of M. pubescens has a higher phenolic content than the aqueous extract. The supplementation of M. pubescens (5 % w/w) to HFD rats decreased significantly (p<0.01) body weight, liver and epididymal adipose tissue relative weights, glycemia, triglycerides (TG), insulin resistance, liver markers, TNF-α, malondialdehyde (MDA), protein carbonyl (PCO), advanced oxidation protein products (AOPP) level, and increased reduced glutathione (GSH) level, glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), and catalase activities as well as ameliorated histological alterations through the reduction hepatic lipid deposition and adipocytes hypertrophy compared to the HFD group. We conclude that M. pubescens powder may be effective for correcting hyperglycemia, hypertriglyceridemia, insulin resistance, and liver markers while decreasing inflammation and oxidative stress in the liver of high-fat diet-fed rats.

Establishment of biochemical and hematological profiles of dromedary camel (Camelus dromedarius) under extensive and intensive production systems.

In Algeria, camel husbandry is undergoing a shift from a traditional extensive system to one more intensive. Such a move in the production system of the dromedary camel should be well investigated in terms of animal welfare, health, and production status. The main objective of this study was to define and evaluate the effects of production systems on physiological responses (hematological and biochemical parameters) in Sahraoui dromedary camels to understand possible changes caused by intensive livestock farming. We analyzed the biochemical and hematological blood profiles in Sahraoui dromedary camel to evaluate and establish the differential responses to intensive conditions. Blood samples were taken from 41 healthy Sahraoui dromedary camels as part of routine veterinary analysis in southern Algeria. In total, 28 camels were from an intensive production system (IS) fed with formulated concentrate and a supply of minerals, and 13 camels were reared in a traditional extensive system (ES) maintained exclusively on grazing. Animals were of a different sex: 16 males and 25 females, and they were divided into three age categories: less than 4 years, 4 to 8, and above 8 years. All animals were healthy. This enabled us to simultaneously check the effect of age and sex on the blood profiles. Results showed that the production system affected the blood parameters of Sahraoui dromedary camel; as total protein, total cholesterol, and urea concentration were significantly higher in the plasma of animals in the intensive production system (P < 0.05). Glucose, triglycerides, and urea values differed significantly (P < 0.05) in camel plasma between age categories. Sex in our study had no effect on enzyme activities (P > 0.05). However, lactate dehydrogenase (LDH) was significantly higher (P < 0.05) in camels reared in the extensive production system (1454.13 ± 290 IU/l) than those reared in intensive production system (1313.17 ± 32 IU/l). Age and sex had a marginal effect on mineral status in dromedary camel plasma as only iron concentrations were significantly higher in male camels (P < 0.05), while Ca, P, and K concentrations were increased in the intensive system. On the other hand, our findings showed that sex, age, and production system did not affect the hematological parameters of Sahraoui dromedary (P > 0.05), except for hematocrit (PCV %) that was significantly higher in the extensive system. This work contributes to a better understanding of Sahraoui dromedary camel biology regarding the effect of different production systems on hemato-biochemical parameters.