RESUMO
Coagulase negative Staphylococci are now being increasingly recognised as pathogens. Some strains produce a viscous extracellular material or slime. These strains are uniquely adapted for adherence to even smooth surfaces. Present study is a preliminary report of 101 isolates of coagulase negative Staphylococci from different clinical specimens. Forty three of these 101 isolates (42.5%) were slime producers. The percentage of slime producing Staphylococci ranged from 20% in peritoneal fluid to 66.6% in Cerebrospinal fluid. The test for slime production may have an important application in deciding the pathogenecity of the strains of coagulase negative Staphylococci and should be done routinely in a diagnostic laboratory.
Assuntos
Polissacarídeos Bacterianos/biossíntese , Infecções Estafilocócicas/microbiologia , Staphylococcus/metabolismo , Técnicas Bacteriológicas , Coagulase/metabolismo , Humanos , Infecções Estafilocócicas/diagnóstico , Staphylococcus/isolamento & purificação , Staphylococcus/patogenicidadeRESUMO
PIP: An enzyme immunoassay for chlamydial antigen was tried in a pilot test of 40 patients with nongonococcal urethritis, 26 with pelvic inflammatory disease and 20 healthy controls. The EIA was a kit from Pharmacia Diagnostics. Endocervical or endourethral swabs with sticks supplied with the kit were inserted in transport tubes and kept in diluent for 10-15 minutes, the tubes were boiled 10-15 minutes, cooled and stored frozen until tested. Aliquots of the specimens and controls were incubated in kit test wells at 37 degrees C. for 30 minutes, enzyme conjugate was added for a further incubation of 45 minutes, wells were washed, and incubated with substrate for 60 minutes. Results were read on a spectrophotometer. 32.5% of the male cases, 38.5% of the female cases and none of the controls were positive. This method appears to be less costly and time consuming than culture of chlamydia, and therefore useful for screening.^ieng
