RESUMO
UNLABELLED: To study prevalence of polyresistance (PR) among the strains of Helicobacter pylori isolated from patients with chronic gastritis, gastroduodenal ulcer and gastric cancer in Georgia. Material and methods. Biopsies of gastric mucosa and resection material were studied in 206patients with gastroduodenal zone diseases for identification of H. pylori (HP) and PR strains of HP. Results. The number of antibiotics involved in development of polyresistant strains varies from 3 to 8. The greatest number of strains (28.57 +/- 1.06%) develop PR to 5 antibiotics, the least--to 8 antibiotics. CONCLUSION: PR prevalence among HP strains is 35.25 +/- 4.05%. PR is most frequently seen to metronidasol and oxacilline, least frequently--to amoxicilline and clarithromycin.
Assuntos
Antibacterianos/uso terapêutico , Farmacorresistência Bacteriana Múltipla , Duodenopatias/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/efeitos dos fármacos , Gastropatias/microbiologia , Biópsia , Duodenopatias/tratamento farmacológico , Duodenopatias/patologia , Infecções por Helicobacter/tratamento farmacológico , Infecções por Helicobacter/patologia , Helicobacter pylori/isolamento & purificação , Humanos , Técnicas In Vitro , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Gastropatias/tratamento farmacológico , Gastropatias/patologiaRESUMO
The phenotypical differences of H. pylori strains isolated in Georgia have been evaluated. Among H. pylori isolates, these strains have been found to differ from typical ones in the morphology of their colonies, growth under microaerophilic and anaerobic conditions, alpha-hemolysin production, resistance to cephalotin and nalidixic acid. At the same time all the strains under study are no different from typical H. pylori strains in respect of their cell morphology, tinctoral properties, growth at different temperatures and their main biochemical properties.
Assuntos
Gastroenteropatias/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/fisiologia , Anaerobiose , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Cefalotina/farmacologia , Meios de Cultura , Farmacorresistência Bacteriana , República da Geórgia , Helicobacter pylori/isolamento & purificação , Proteínas Hemolisinas/metabolismo , Humanos , Ácido Nalidíxico/farmacologia , Especificidade da EspécieRESUMO
The distribution of plasmids in N. meningitidis strains according to their origin and serological groups has been studied. Plasmids have been discovered in N. meningitidis of all groups, plasmid-carrying strains constituting 55% of strains isolated from healthy carriers and 46.2% of strains isolated from patients. The molecular weight of N. meningitidis plasmid DNA varies from 2.9 MD to 95 MD.
Assuntos
Neisseria meningitidis/genética , Plasmídeos/genética , Portador Sadio/microbiologia , DNA Bacteriano/análise , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Eletroforese em Gel de Ágar , Humanos , Meningite Meningocócica/microbiologia , Peso Molecular , Nasofaringe/microbiologia , Neisseria meningitidis/química , Neisseria meningitidis/classificação , Neisseria meningitidis/isolamento & purificação , Plasmídeos/análise , Plasmídeos/isolamento & purificação , SorotipagemRESUMO
In Neisseria meningitidis strains isolated from patients with meningococcal infection and from carriers, the presence of plasmids has been detected by the modified method of H. C. Bernboim and J. Doly. The strains have been studied with respect to their pathogenicity and sensitivity to antibacterial substances. No correlation between the presence of plasmids and pathogenicity factors, such as antilysozyme activity, the presence of hyaluronidase and the positive reaction to Congo red, resistance to penicillin, serogroup and the sources of isolation, has been established.
