Bacterial cellulose biosynthesis: Optimization strategy using iranian nabat industry waste.

Bacterial cellulose (BC) is a biopolymer has found extensive applications across different fields due to its nanostructure and biomaterial performance. This study focused on optimizing yield of BC produced by Komagataeibacter xylinus CH1, isolated from kombucha SCOBY. The study aimed to use Nabat industry waste (NIW) as a cost-effective alternative carbon source for submerged fermentation. To optimize the fermentation criteria, the central composite design was used with the inoculation amount (1.5-4.5 % VV-1), NIW (0-1%), and fermentation time (3-7 days) as independent variables. The impressive results indicated the yield was enhanced up to 45.543 gL-1 at 3.013 % VV-1 of inoculation, 0.516 % NIW, and 7 days of stirred fermentation. SEM, XRD, FTIR, and TGA were applied to evaluate the characteristics of freeze-dried BC, such as the three-dimensional, porous structure, crystalline peaks, amorphous haloes, and thermal stability. The physicochemical properties of BC including high moisture content (93.022 ± 0.472 %), water absorption rate (569.473 ± 3.739 %), water-holding capacity (1333.016 ± 3.680 %), porosity (166.247 ± 2.055 %), and low water activity (0.296 ± 0.030 %) were achieved. Rheological properties of BC suspensions showed that G' dominated over G″, with tan δ values lower than 1. These characteristics indicate NIW and stirred fermentation conditions are a promising method for producing BC in high yield.

Exploring the Diversity and Potential Use of Flower-Derived Lactic Acid Bacteria in Plant-Based Fermentation: Insights into Exo-Cellular Polysaccharide Production.

Isolation of new plant-derived lactic acid bacteria (LAB) is highly prioritized in developing novel starter cultures for plant-based fermentation. This study explores the diversity of LAB in Danish flowers and their potential use for plant-based food fermentation. A total of 46 flower samples under 34 genera were collected for LAB isolation. By introducing an enrichment step, a total of 61 LAB strains were isolated and identified using MALDI-TOF and 16S rRNA sequencing. These strains represent 24 species across 9 genera, predominantly Leuconostoc mesenteroides, Fructobacillus fructosus, Apilactobacillus ozensis, and Apilactobacillus kunkeei. Phenotypic screening for exo-cellular polysaccharide production revealed that 40 strains exhibited sliminess or ropiness on sucrose-containing agar plates. HPLC analysis confirmed that all isolates produced exo-cellular polysaccharides containing glucose, fructose, or galactose as sugar monomers. Therefore, the strains were glucan, fructan, and galactan producers. The suitability of these strains for plant-based fermentation was characterized by using almond, oat, and soy milk. The results showed successful acidification in all three types of plant-based matrices but only observed texture development in soy by Leuconostoc, Weissella, Lactococcus, Apilactobacillus, and Fructobacillus. The findings highlight the potential of flower-derived LAB strains for texture development in soy-based dairy alternatives.