RESUMO
The effects of chronic intranasal administration of 300 nmol/kg obestatin and its fragment FNAP-NH2 on behavioral activity and nociceptive threshold were examined in male Wistar rats with normal body weight or alimentary obesity. In normal rats, obestatin produced no effect on behavior and nociception, whereas FNAP-NH2 fragment enhanced risk-taking behavior. Rats with excess body weight demonstrated less pronounced risk-taking behavior and elevated nociceptive threshold in comparison with normal animals, but these differences were abolished by chronic administration of FNAP-NH2.
Assuntos
Nociceptividade/efeitos dos fármacos , Oligopeptídeos/farmacologia , Limiar da Dor/efeitos dos fármacos , Hormônios Peptídicos/farmacologia , Administração Intranasal , Sequência de Aminoácidos , Animais , Ansiedade/patologia , Ansiedade/fisiopatologia , Peso Corporal , Ingestão de Alimentos/fisiologia , Masculino , Nociceptividade/fisiologia , Obesidade/patologia , Obesidade/fisiopatologia , Limiar da Dor/psicologia , Ratos , Ratos Wistar , Assunção de RiscosRESUMO
We studied the effects of anorectic peptide obestatin and its fragment (1-4) on the antioxidant defense system in animals with normal and experimentally induced increased body weight. In rats with normal body weight, no changes in activity of the antioxidant defense system 1 week after single administration of the substances. After chronic administration of obestatin and fragment (1-4) for 1 week, total antioxidant capacity of the plasma decreased; obestatin also lowered the content of TBA-reactive products. In the overweight rats, SOD-like activity in the plasma increased 1 week after chronic administration of obestatin. Hence, obestatin and its fragment (1-4) induced changes in the antioxidant defense system only after chronic administration.
Assuntos
Antioxidantes/análise , Depressores do Apetite/farmacologia , Sobrepeso/tratamento farmacológico , Fragmentos de Peptídeos/farmacologia , Hormônios Peptídicos/farmacologia , Sequência de Aminoácidos , Animais , Depressores do Apetite/administração & dosagem , Depressores do Apetite/uso terapêutico , Catalase/sangue , Dieta Hiperlipídica/efeitos adversos , Esquema de Medicação , Avaliação Pré-Clínica de Medicamentos , Masculino , Dados de Sequência Molecular , Sobrepeso/sangue , Sobrepeso/etiologia , Fragmentos de Peptídeos/administração & dosagem , Hormônios Peptídicos/administração & dosagem , Hormônios Peptídicos/uso terapêutico , Ratos , Ratos Wistar , Superóxido Dismutase/sangueAssuntos
Glicemia/metabolismo , Ingestão de Líquidos/efeitos dos fármacos , Ingestão de Alimentos/efeitos dos fármacos , Grelina/farmacologia , Motivação , Animais , Regulação do Apetite , Grelina/administração & dosagem , Grelina/química , Masculino , Fragmentos de Peptídeos/farmacologia , Ratos , Ratos WistarRESUMO
We studied the effects of the anorexigenic peptide obestatin on the coagulation system and blood rheology (by the parameters of platelet aggregation and osmotic resistance of erythrocytes) in vitro and in vivo. Obestatin inhibited in vitro platelet aggregation in the entire dose range and reduced osmotic resistance of erythrocytes in all doses except 300 nmol/kg (obestatin in a dose of 300 nmol/kg had no effect on this parameter). Similar to the results of in vitro experiments, intranasal, intraperitoneal, and subcutaneous administration of obestatin in a dose of 300 nmol/kg inhibited platelet aggregation and had no effect on the osmotic resistance of erythrocytes.
Assuntos
Pressão Osmótica/efeitos dos fármacos , Hormônios Peptídicos/metabolismo , Agregação Plaquetária/efeitos dos fármacos , Difosfato de Adenosina/metabolismo , Animais , Apetite/efeitos dos fármacos , Coagulação Sanguínea/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Masculino , Óxido Nítrico/biossíntese , RatosAssuntos
Peso Corporal/efeitos dos fármacos , Ingestão de Líquidos/efeitos dos fármacos , Grelina/farmacologia , Administração Intranasal , Animais , Grelina/administração & dosagem , Grelina/análogos & derivados , Masculino , Fragmentos de Peptídeos/administração & dosagem , Fragmentos de Peptídeos/farmacologia , Ratos , Ratos WistarRESUMO
Single exposure of white outbred rats to electromagnetic radiation with a frequency 905 MHz (GSM frequency) for 2 h increased anxiety, reduced locomotor, orientation, and exploration activities in females and orientation and exploration activities in males. Glucocorticoid levels and antioxidant system activity increased in both males and females. In addition to acute effects, delayed effects of radiation were observed in both males and females 1 day after the exposure. These results demonstrated significant effect of GSM-range radiation on the behavior and activity of stress-realizing and stress-limiting systems of the body.
