[The current objective criteria for the adequacy of general anesthesia in children]. / Sovremennye ob''ektivnye kriterii adekvatnosti obshchei anestezii u detei.

Auditory evoked potentials (AEP) were recorded and analyzed in 12 children during general halothane and ketamine anesthesia. Ketamine suppressed the amplitude of primary negative component and eliminated secondary AEP components with latencies more than 60 ms, including N-200 and P-300 waves. By contrast, halothane did not affect primary components with less than 95 ms latencies and suppressed the latest components. Hence, ketamine suppresses the nonspecific afferent pathways and the brain structures responsible for memory and emotional functions, while halothane does not affect the afferent pulsation and partially suppresses emotional and mnemonic functions.

[Use of electroblotting as a method for preparing samples of proteins and their fragments for microsequencing]. / Primenenie élektroblottinga v kachestve metoda polucheniia obraztsov belkov i ikh fragmentov dliia mikrosekvenirovaniia.

Gel electrophoresis in the presence of sodium dodecyl sulphate followed by electroblotting was employed in sample preparation for microsequencing proteins and protein fragments. Three types of solid supports were compared: glass fiber filters modified by aminopropyltriethoxysilane or covered with polybrene, and polyvinylidenedifluoride membranes. N-Terminal amino acid sequences of several proteins (Mr 14-140 kDA were determined on a gas-phase sequencer with the standard programme; 20-200 pmoles of the protein can be assayed by this method.