RESUMO
Topoisomerase II gene of Leishmania genus was used to develop a molecular tool for detection and species differentiation of Leishmania from clinical samples. Identification was achieved by a polymerase chain reaction followed by digestion with 2 restriction endonucleases BstU1 and Taq1. Despite the relatively low sensitivity, it is able to differentiate between 3 complexes responsible for cutaneous leishmaniasis.
Assuntos
DNA Topoisomerases Tipo II/genética , Leishmania/classificação , Leishmaniose Cutânea/diagnóstico , Leishmaniose/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase/métodos , Polimorfismo de Fragmento de Restrição , Sequência de Bases , DNA de Protozoário/análise , DNA de Protozoário/genética , Humanos , Leishmania/genética , Leishmania/isolamento & purificação , Leishmania infantum/classificação , Leishmania infantum/genética , Leishmania infantum/isolamento & purificação , Leishmania major/classificação , Leishmania major/genética , Leishmania major/isolamento & purificação , Leishmaniose/parasitologia , Leishmaniose Cutânea/parasitologia , Sensibilidade e Especificidade , Análise de Sequência de DNA , Especificidade da Espécie , TunísiaRESUMO
A 62-year-old woman was referred to the dermatology department for a history of fever, asthenia and cutaneous rash, which appeared after a 3-day course of digitalin and acenocoumarol for atrial fibrillation. The physical examination revealed multiple round confluent purpuric lesions over her entire legs with no blistering. Laboratory exams were all negative. Biopsy of the involved skin was compatible with leucocytoclastic vasculitis. The acenocoumarol treatment was withheld and the skin lesions resolved spontaneously over the next 10 days. The cause of this purpura was seemingly acenocoumarol because of the close temporal relationship between exposure to the drug and the onset of the symptoms, and the spontaneous resolution of the lesions after acenocoumarol was discontinued. This observation illustrates a rare association between vasculitis and acenocoumarol. Clinicians should be aware of this potential adverse effect and recommend interrupting the drug intake when temporal relation is evocative.