RESUMO
Mutations in the human sulphate transporter gene, DTDST, have been implicated in several diseases. Analysis of affected patients has linked disease symptoms to faulty sulphate transporter activity. We have reproduced two of these mutations in SHST1, a homologous member of the family isolated from the tropical legume, Stylosanthes hamata. Both mutations significantly reduce sulphate transport activity of SHST1. These results indicate that conserved residues between distinct members of the family may share essential roles in structure or function. The results also suggest that putative helix 9 may be important for stability and/or trafficking of SHST1 to the plasma membrane.
Assuntos
Fabaceae , Mutação/genética , Plantas Medicinais , Sequência de Aminoácidos , Substituição de Aminoácidos/genética , Proteínas de Transporte de Ânions , Transporte Biológico , Western Blotting , Proteínas de Transporte/química , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Membrana Celular/metabolismo , Sequência Conservada/genética , Teste de Complementação Genética , Humanos , Cinética , Proteínas de Membrana Transportadoras , Dados de Sequência Molecular , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/metabolismo , Alinhamento de Sequência , Relação Estrutura-Atividade , Transportadores de Sulfato , TermodinâmicaRESUMO
PIP: During the year 1980, 96 (56.7%) of late fetal deaths and 73 (43.3%) of early neonatal deaths constituted the total perinatal loss among 4074 consecutive deliveries of babies with birthweight more than 1 kg at Main Hospital, Bhilai. The PMR was calculated at 41.4/1000 births. PMR was 73.3 in unbooked cases as compared to 37.8 in booked ones; 90.6 in abnormal deliveries as compared to 32.7 in normal ones; 184.2 in multiple pregnancies as compared to 38.8 in singleton pregnancies; and 241.9 in breech presentation births as compared to 56.0 and 57.0 in LSCS and vacuum deliveries. LBW babies constituted almost 1/3 (32.1%) of the total births. The PMR was 331.0 in perinates with birthweight less than 2 kg as compared to 29.3 in perinates weighing more than 2 kg. Locally existing demographic factors, excellent antenatal care, and an adequate standard of perinatal service are the 3 factors responsible for the low PMR in steel plant hospitals when compared to the teaching hospitals.^ieng
