RESUMO
Examinations of the rat kidney metabolism were carried out by histological (staining with haematoxylin and eosin), histochemical (response to lactate and succinic dehydrogenases activities), and electrophoretic methods (separation of isoenzymes of lactate dehydrogenase) after treating animals with the sulphonamide Biseptol 480, for 7 (consecutive) d by stomach tube. Biseptol given to the animals exceeded 10 times human therapeutical dose. It was found out that Biseptol 480, applied to the animals with healthy urinary system, does not pathologically disturb the examined stages of the metabolic processes in the rat's kidney.
Assuntos
Rim/metabolismo , Oxirredutases/metabolismo , Sulfametoxazol/farmacologia , Trimetoprima/farmacologia , Animais , Combinação de Medicamentos/farmacologia , Eletroforese em Gel de Poliacrilamida , Isoenzimas , Rim/efeitos dos fármacos , L-Lactato Desidrogenase/isolamento & purificação , L-Lactato Desidrogenase/metabolismo , Ratos , Succinato Desidrogenase/metabolismo , Combinação Trimetoprima e SulfametoxazolRESUMO
White female rats were daily treated with Fenoterol in a dose of 1.8 mg/kg of body weight beginning with the 10th d till the end of gestation. Cell cultures were made by using the skin of mature foetuses. Fibroblast-like cells of primary and passaged cultures were examined. The slices were stained by Giemsa's reagent and examined with Brachet's reaction to ribonucleic acid. Histological reactions to acid phosphatase activity, succinic and lactic dehydrogenases demonstrated changes showing disturbed metabolism of the examined cells.