RESUMO
The incorporation of neutral [70]fullerenes (C70) led to bicelle formation in a relatively low lipid concentration range from neutral lipid mixtures (DMPC/DHPC). Furthermore, C70 addition resulted in the formation of large bicelles with a radius of ca. 100 nm, in contrast to C70-free bicelles that were formed from anionic lipid mixtures (DMPC/DHPC/DMPG). The stabilization of these bicelles was attributed to C70 incorporation into the membranes.
Assuntos
Fulerenos/química , Fosfolipídeos/análise , Fosfolipídeos/química , Simulação de Dinâmica Molecular , Estrutura Molecular , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
Lipid-membrane-incorporating C(60) and C(70) (LMIC(60) and LMIC(70)) were prepared by the fullerene-exchange reaction from the γ-cyclodextrin cavity to vesicles (we call this method the "exchange method"). An advantage of this method is that the ratios of [C(60)]/[lipids] and [C(70)]/[lipids] can be arbitrarily controlled by adjusting the ratios of the fullerenes and liposome. The maximum ratio (30 mol%) obtained was approximately 14 and 100 times higher than those achieved for LMIC(60) and LMIC(70) , respectively, that were prepared by the classical method, which we call the "premixing method" (dissolving lipids and C(60) or C(70) in chloroform, followed by concentration and extraction with water). Furthermore, the stabilities and photodynamic activities of the LMIC(60) and LMIC(70) solutions prepared by the exchange method were shown to be much higher than those prepared by the premixing method. That is, the exchange method was found to be superior to the premixing method as a preparative method of LMIC(60) and LMIC(70) for applications in photomedical and photomaterials chemistry.
Assuntos
Fulerenos/química , Lipídeos/química , Membranas Artificiais , Clorofórmio/química , Ciclodextrinas/química , Estrutura Molecular , Água/químicaRESUMO
We confirmed that most C(60) fullerene units are located in the hydrophobic core of the lipid bilayer membrane in water-soluble lipid membrane incorporated C(60) (LMIC(60)) complexes using differential scanning calorimetry (DSC) and (13)C NMR spectra in the presence of radical labels.
