RESUMO
This review discusses the available data on molecular mechanism of penetration of baculoviruses into cell and structural features of proteins involved in this process. These data are discussed in terms of basic postulates of contemporary model of fusion of biological membranes and other large DNA-containing viruses.
Assuntos
Baculoviridae/fisiologia , Insetos/virologia , Internalização do Vírus , Animais , Membrana Celular/metabolismo , Membrana Celular/virologia , Herpesviridae/fisiologia , Poxviridae/fisiologia , Proteínas Virais de Fusão/metabolismo , Proteínas Virais de Fusão/ultraestruturaRESUMO
Genomes of some positive-strand RNA viruses do not contain cap-structure, but instead their 5'-end is covalently linked to a viral protein called VPg. Complex formation between VPg and cellular translation initiation factors (eIFs) has been extensively studied in the context of the model of this complex involvement in virus mRNA translation initiation and cellular protein translation shut down in infected cells. The potato virus (PVY) VPg was expressed in bacterial and baculovirus systems in order to investigate its binding capacity to wheat eIF4E and its isoform. Both purified recombinant eIF4E and eIF(iso)4E were identified in vitro as binding partners of the purified recombinant VPg by using affinity chromatography, as well in vivo by coexpressing of recombinant VPg and eIFs in insect cells with following complex purification using affinity chromatography. Besides it was shown that PVY VPg also formed a complex with endogenous insect eIF4E in vivo. PVY VPg interaction with eIF4E of wheat (non permissive plant for PVY), and also with so evolutionary distant partner as insect eIF4E suggests the conservation of general structural features of eIF4E implicated in the formation of the complex with VPg.
Assuntos
Fator de Iniciação 4E em Eucariotos/genética , Genoma Viral , Potyvirus/genética , Proteínas Recombinantes/genética , Ribonucleoproteínas/genética , Proteínas não Estruturais Virais/genética , Animais , Baculoviridae/genética , Baculoviridae/metabolismo , Cromatografia de Afinidade , Escherichia coli/genética , Escherichia coli/metabolismo , Plasmídeos , Potyvirus/metabolismo , Ligação Proteica , Solanum tuberosum/metabolismo , Solanum tuberosum/virologia , Spodoptera/metabolismo , Spodoptera/virologia , Triticum/metabolismoRESUMO
The results of the creation of new expressive system on Malacosoma neustria nuclear polyhedrosis virus and cell line originated from Antheraea pernyi tissues was demonstrated. The expression of bacterial beta-galactosidase-coding gene was analyzed in this system.
