Gladiolus Rust Caused by Uromyces transversalis Makes First Nearctic Appearance in Florida.

The most serious rust pathogen of gladiolus (Gladiolus × hortulanus), Uromyces transversalis, has been listed as an exotic pathogen of concern for the United States for more than 80 years (4). Native to South Africa, the pathogen was reported in the Western Hemisphere for the first time in Brazil (2) and Argentina (1). Reports of gladiolus rust in several central Mexican states from 2004 to 2005 (3; http://www.pestalert.org/espanol/oprDetail.cfm?oprID=138 ) and interceptions at Mexican border stations and in Brazilian imports in 2005 at the port of Miami, FL collectively raised the alert level in the United States to high. In April 2006, the Hawaii Department of Agriculture notified the USDA of rust-infected gladiolus in a cut-flower shipment that was traced back to a 1,400-acre (565 ha) farm in Manatee County, FL. Inspection at the farm yielded samples that were quickly confirmed as U. transversalis by FDACS-DPI and USDA plant pathologists. The disease was identified in eight residential gardens near the commercial find and in another 700-acre (285 ha) farm in remote Hendry County, 100 miles to the southeast. In May 2006, gladiolus rust was detected in residential and commercial gladiolus in San Diego County, CA (see companion publication). On the advice of a USDA-assembled panel of experts, strict rust management guidelines and fallow host-free periods were implemented with the ultimate goal of eradication. Subsequent summer, fall, and now winter surveys in the infested commercial and residential areas have uncovered diminishing amounts of rust, with last traces detected on 9 September 2006. Commercial planting resumed at both farms in late summer, and crops remained rust free under weekly inspection until 15 February 2007 in Manatee County and 29 March 2007 in Hendry County. To insure a rust-free product, cut flowers are carefully inspected and foliage stripped at the packinghouse. Eradication will be attempted once more with a fallow host-free period before the 2007 season. U. transversalis is an autoecious rust that mainly infects Gladiolus spp., but has been known to infect other members of the Iridaceae: Anomatheca, Crocosmia, Melasphaerula, Tritonia, and Watsonia. Amphigenous uredinia form in transverse lines across gladiolus foliage and also on flower spikes under heavy disease pressure. The isolate present in Florida fits the literature description of U. transversalis in every respect (uredinia 0.5 to 1.5 mm in diameter, subglobose to ellipsoid verruculose yellow-amber urediniospores, 15 to 28 × 14 to 20 µm with wall 1.5 to 2.5 µm thick; telia also amphigenous, 0.5 to 1.3 µm in diameter, dark brown-black, subglobose to pyriform smooth amber teliospores, 20 to 30 × 15 to 20 µm with wall 1.5 to 2.0 µm thick, 4 to 6 µm thick at apex, pale brown to hyaline pedicel 30 to 40 µm long, yellow-brown paraphyses in pustule) ( http://nt.ars-grin.gov/fungaldatabases/new_allView.cfm?whichone=all&thisName=Uro myces%20transversalis&organismtype=Fungus ). Urediniospores initiated typical foliar lesions on transplanted gladiolus samples kept in the FDACS-DPI quarantine greenhouse during the diagnostic process. References: (1) J. R. Hernandez and J. F. Hennen. Sida 20:313, 2002. (2) G. P. B. Pitta et al. Biologica 47:323, 1981. (3) G. Rodriguez-Alvarado et al. Plant Dis. 90:687, 2006. (4) J. A. Stevenson. Page 82 in: Foreign Plant Diseases. USDA Fed. Hortic. Board Bureau Plant Ind. Government Printing Office, Washington DC, 1926.

First Report of a Rust Disease on Ohia Caused by Puccinia psidii in Hawaii.

Several species of Metrosideros (Myrtaceae), referred to as ohia in Hawaii, are endemic trees that comprise as much as 80% of the native Hawaiian forests. For centuries, these trees have provided niches for many indigenous and endangered plants and animals and are treasured by Hawaiians for their beauty and role in folklore and legends. During April 2005, a cultivated ohia plant was diagnosed by the Agricultural Diagnostic Service Center at the University of Hawaii at Manoa as infected by a rust fungus. Rust pustules containing abundant urediniospores were observed on leaves, stems, and sepals, causing discolored spots and severe deformity of young leaves and growing tips. By July 2005, a similar rust disease was observed on other plants in the family Myrtaceae; namely Syzygium jambos (L.) Alston, Eugenia koolauensis Degener, E. reinwardtiana (Blume) DC, and Psidium guajava L. Microscopic examination of the uredinia and urediniospores showed that the rust was morphologically similar to Puccinia psidii, which is reported as the guava or eucalyptus rust in Florida and Central and South America (1,2). To confirm the identity of this fungus, DNA was extracted from urediniospores of two isolates collected from ohia plants, and their nuclear ribosomal internal transcribed spacer (ITS) was amplified with two universal primers, ITS4 and ITS5 (3). Sequences of the ITS region of these isolates from ohia were identical to the P. psidii isolates provided by A. Alfenas in Brazil and M. Rayachhetry in Florida. Koch's postulate of the isolates, obtained from ohia, was performed using 1 × 108 spores/ml of urediniospores suspension in distilled water. The suspension was sprayed onto 6-month-old ohia seedlings. These inoculated seedlings were placed in clear plastic chambers maintained at 100% relative humidity and 22°C with a combination of 10-h fluorescent light period and a 14-h dark period. After 48 h of incubation, the seedlings were removed from the chambers and transferred to a greenhouse where the ambient temperature ranged from 20 to 24°C. Rust pustules appeared after 1 to 2 weeks of incubation. Symptoms first appeared as tiny, bright yellow, powdery eruptions that developed into circular, uredinial pustules on the stem and foliage. These pustules later expanded, coalesced, and became necrotic, spreading over the entire leaf and stem surfaces, and then leaves and stems were deformed and tip dieback ensued. These symptoms were the same as those observed on the naturally infected cultivated ohia plant mentioned above. P. psidii is reported to be native to South and Central America that later spread to some Myrtaceous plants in the Caribbean countries (1). It has a very wide host range within the family Myrtaceae (2). To our knowledge, this is the first report of P. psidii in Hawaii. This rust disease may pose a formidable threat to Myrtaceous species that make up the native Hawaiian forests and are grown as ornamental plants or for the production of wood chips. References: (1) T. A. Coutinho et al. Plant Dis. 82:819. 1998. (2) M. B. Rayachhetry et al. Biol. Control 22:38. 2001. (3) T. J. White et al. Page 315 in: PCR Protocols. M. A. Innis et al., eds. 1990.

Evaluation of Colletotrichum gloeosporioides for Biological Control of Miconia calvescens in Hawaii.

Miconia calvescens (Melastomataceae), from the Neotropics, is a noxious forest weed in Hawaii. We evaluated an isolate of Colletotrichum gloeosporioides that causes leaf spots on Miconia spp. in Brazil for its potential in biological control. Hawaii has no native Melastomataceae genera but does have members of 12 introduced genera. Following Wapshere's centrifugal phylogenetic method (2), eight species of Melastomataceae genera in Hawaii were inoculated in addition to Miconia spp. Naturalized and native Hawaiian members of the order Myrtales also were inoculated to determine host specificity, including Terminalia catappa (Combretaceae); Cuphea hysopifolia and C. ignea (Lythraceae); Arthrostema ciliatum, Clidemia hirta, Dissotis rotundifolia, Heterocentron subtriplinervium, Medinilla scortechenii, Melastoma candidum, Pterolepsis glomerata, and Tibouchina herbaceae (Melastomataceae); Eucalyptus grandis, Eucalyptus microcorys, Eugenia reinwardtiana, Eugenia uniflora, Leptospermum laevigatum, Melaleuca quinquenervia, Metrosideros polymorpha, Psidium guajava, and Syzgium malaccanse (Myrtaceae); Fuchsia magellanica and Oenothera stricta (Onagraceae); and Wikstroemia oahuensis and W. uva-ursi (Thymelaeaceae). All M. calvescens plants were grown from seed collected in Hawaii. Other test plants were grown from seeds or cuttings in artificial potting medium in a greenhouse. Plants had 6 to 8 mature leaves when inoculated. C. gloeosporioides was cultured on 10% potato dextrose agar supplemented with plain agar (35 g/liter) and incubated under constant fluorescent illumination at 20°C. Conidia were harvested by flooding 10-to 14-day-old cultures with sterile tap water, followed by light scraping with a scalpel. Conidial suspensions were adjusted to 106 conidia per ml and applied to both leaf surfaces with a hand-held sprayer. Inoculated plants were kept at 100% relative humidity and 16 to 25°C for 48 h. Four replicate plants and one plant of M. calvescens per species were inoculated. Plants were observed for symptom development for up to 6 weeks. The entire test was repeated once. Lesions were visible after 7 to 10 days. Young lesions had chlorotic halos and expanded in a roughly circular pattern to diameters of 5 to 10 mm. Mature lesions developed necrotic centers, coalesced, and became dry and brittle with age, resulting in extensive leaf necrosis. Defoliation of moderately to severely infected leaves occurred ≈ 30 days after inoculation. With the exception of M. calvescens, C. gloeosporioides did not produce visible symptoms on test plants. The failure of Clidemia hirta, the taxonomic species most closely related to M. calvescens, to become symptomatic was particularly significant relative to the centrifugal phylogenetic concept. The results demonstrate that our pathogen (VIC 19306) is distinct from C. gloeosporioides f. sp. clidemiae (1), which did not infect M. calvescens. We designate our pathogen C. gloeosporioides f. sp. miconiae. Voucher specimens (VIC 19306, Sana, RJ, 24.II.1998, and R. W. Barreto) and cultures are maintained at the Departamento de Fitopatologia, Universidade Federal de Viçosa MG, Brazil. References: (1) E. E. Trujillo et al. Plant Dis. 70:974, 1986. (2) A. J. Wapshere. Ann. Appl. Biol. 77:201, 1974.