Recombinant Lactococcus starters as a potential source of additional peptidolytic activity in cheese ripening.

AIMS: The aim of this study was to modulate the lactococcal proteolytic system for enhancement of the cheese ripening process. METHODS AND RESULTS: The genes encoding PepN, PepC, PepX and PepI peptidases of a highly proteolytic Lactobacillus helveticus strain were transferred into Lactococcus lactis in a food-grade cloning system. A comparison of the relative peptidase activities from the transformants with those from the untransformed host, determined in the conditions of maturing cheese, showed that an increase in peptidase activity could be achieved by introducing a selected peptidase gene from Lact. helveticus into L. lactis. CONCLUSIONS: Recombinant L. lactis starter strains, carrying a peptidase gene from Lact. helveticus, may have an important contribution to the proteolysis of maturing cheese by producing an additional peptidolytic enzyme activity. SIGNIFICANCE AND IMPACT OF THE STUDY: The results will be of importance in shortening the ripening period and production of special cheeses (e.g. reduced-fat cheeses) with improved characteristics.

Use of serum biochemistry to evaluate nutritional status and health of incubating common eiders (Somateria mollissima) in Finland.

During 1997-1999, we collected serum samples from 156 common eider (Somateria mollissima) females incubating eggs in the Finnish archipelago of the Baltic Sea. We used serum chemistry profiles to evaluate metabolic changes in eiders during incubation and to compare the health and nutritional status of birds nesting at a breeding area where the eider population has declined by over 50% during the past decade, with birds nesting at two areas with stable populations. Several changes in serum chemistries were observed during incubation, including (1) decreases in serum glucose, total protein, albumin, beta-globulin, and gamma-globulin concentrations and (2) increases in serum uric acid, creatine kinase, and beta-hydroxybutyrate concentrations. However, these changes were not consistent throughout the 3-yr period, suggesting differences among years in the rate of carbohydrate, lipid, and protein utilization during incubation. The mean serum concentrations of free fatty acids, glycerol, and albumin were lowest and the serum alpha- and gamma-globulin levels were highest in the area where the eider population has declined, suggesting a role for nutrition and diseases in the population dynamics of Baltic eiders.

The burned hand. Early treatment and surgery of scars and contractions.

Hand burns are common injuries in which early accurate diagnosis of the severity of the injury and active surgical treatment can save it or diminish the permanent disability caused by the thermal trauma. During the four year period of 1976--1979 we treated 45 patients with acute hand burns; secondary surgery was performed in 29 scarred and contracted hands. Deep dermal or subdermal (III degree) burns were either immediately excised to the vital tissue (within 6 hr postburn) or early afterwards (within 1--7 days post injury). The reconstruction of the excised areas was made by free skin grafting in 43 hands. If deep structures were exposed, subsequent reconstructions were used: local, groin, or free flaps. The aims in treatment of scarred hands were to restore function and to repair unstable scars. The following secondary procedures, often combined in a one-stage operation, were performed: scar excision and regrafting, web space correction, groin flap reconstruction, digital joint arthrodesis or capsulectomy, tendon transfer, and spherical osteotomy.